FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4280543669> ?p ?o ?g. }

• W4280543669 endingPage "162" @default.
• W4280543669 startingPage "158" @default.
• W4280543669 abstract "To investigate the protective effect of Echinococcus granulosus hydatid cyst fluid protein (HCFP) on ovalbumin (OVA)-induced allergic rhinitis (AR) in mice.Twenty-four BALB/c mice at ages of 8 to 10 weeks, each weighing approximately 20 g, were randomly divided into four groups, including groups A (blank control group), B (blank intervention group), C (AR model group) and D (AR+HCFP intervention group), with 6 mice in each group. On days 0, 2, 4, 6, 8, 10 and 12, mice in groups A, B, C and D were injected with 200 μL sterile phosphate buffered saline (PBS), 200 μL sterile PBS containing 20 μg HCFP, 200 μL sterile PBS containing 50 μg OVA and 5 mg Al(OH)3 gel, and 200 μL sterile PBS containing 50 μg OVA, 5 mg Al(OH)3 gel and 20 μg HCFP, respectively. On days 14 to 20, mice in groups A, B, C and D were administered with 40 μL sterile PBS, 40 μL sterile PBS containing 20 μg HCFP, 40 μL sterile PBS containing 2 mg OVA and 40 μL sterile PBS containing 2 mg OVA and 20 μL HCFP by nasal drop, respectively. Mouse behavioral changes were observed and behavioral scores were estimated. The serum levels of interferon-γ (IFN-γ), interleukin-4 (IL-4), IL-5, IL-10, transforming growth factor-β (TGF-β) and OVA-specific IgE antibody (OVA-sIgE) were measured using enzyme-linked immunosorbent assay (ELISA), and the pathological changes of mouse nasal mucosa were observed by hematoxylin and eosin (HE) staining.The mean behavioral score was significantly greater in Group C (6.83 ± 0.50) than in groups A (1.17 ± 0.52) and B (1.33 ± 0.52) (P < 0.05), while a lower mean behavioral score was estimated in Group D (3.50 ± 0.50) than in Group C (P < 0.05). There were significant differences among the groups in terms of serum IFN-γ (F = 4.08, P < 0.05), IL-4 (F = 275.90, P < 0.05), IL-5 (F = 96.82, P < 0.05), IL-10 (F = 77.67, P < 0.05), TGF-β (F = 9.98, P < 0.05) and OVA-sIgE levels (F = 44.69, P < 0.05). The serum IFN-γ level was significantly lower in Group C than in groups A, B and C (P < 0.05), and the serum levels of IL-4, IL-5 and OVA-sIgE were significantly higher in Group C than in groups A, B and C (P < 0.05), while the serum IL-10 and TGF-β levels were significantly greater in Group D than in Group C (P < 0.05). Microscopy showed apparent loss of nasal mucosa cilia, increased number and enlargement of goblet cells, interstitial edema and submucous vascular dilation in Group C, while the pathological changes of nasal mucosa were alleviated in Group D relative to Group C.E. granulosus HCFP has a protective activity against OVA-induced allergic rhinitis in mice.［摘要］目的 观察细粒棘球蚴囊液蛋白 (hydatid cyst fluid protein, HCFP) 对卵清蛋白 (ovalbumin, OVA) 诱导的小鼠变应性鼻炎 (allergic rhinitis, AR) 的保护作用。方法 将24 只BALB/c小鼠 (8~10周龄, 体质量约20 g) 随机分成A (空白对照组)、B (空白干预组)、C (AR模型组)、D (AR + HCFP干预组) 4组, 每组6只。在基础致敏阶段 (实验第0、2、4、6、8、10、12天), A、B、C、D组小鼠分别腹腔注射200 μL无菌磷酸盐缓冲液 (phosphate buffered saline, PBS), 含20 μg HCFP的无菌PBS溶液, 含50 μg OVA 和5 mg Al(OH)3凝胶的无菌PBS溶液, 含50 μg OVA、5 mg Al(OH)3凝胶和20 μg HCFP 的无菌PBS溶液。在局部激发阶段 (实验第14~20 天), A、B、C、D组小鼠分别鼻腔滴注体积为40 μL的无菌PBS、含20 μg HCFP的无菌PBS、含2 mg OVA的无菌PBS、含2 mg OVA和20 μL HCFP的无菌PBS。观察各组小鼠行为学改变并进行行为学评分, 采用酶联免疫吸附试验 (enzyme-linked immunosorbent assay, ELISA) 检测各组小鼠血清γ干扰素 (interferon-γ, IFN-γ)、白细胞介素4 (interleukin-4, IL-4)、IL-5、IL-10、转化生长因子-β (transforming growth factor-β, TGF-β) 和OVA特异性IgE抗体 (OVA-sIgE) 水平, 采用苏木精-伊红 (HE) 染色观察各组小鼠鼻黏膜病理学改变。结果 C组小鼠行为学评分 [(6.83 ± 0.50) 分]显著高于A组 [(1.17 ± 0.52) 分]和B组 [(1.33 ± 0.52) 分], D组小鼠行为学评分 [(3.50 ± 0.50) 分]较C组降低 (P均 < 0.05)。4组小鼠血清IFN-γ (F = 4.08, P < 0.05)、IL-4 (F = 275.90, P < 0.05)、IL-5 (F = 96.82, P < 0.05)、IL-10 (F = 77.67, P < 0.05)、TGF-β (F = 9.98, P < 0.05) 及OVA-sIgE水平 (F = 44.69, P < 0.05) 差异均有统计学意义。C组小鼠血清IFN-γ水 平低于A、B组和C组, 且血清IL-4、IL-5、OVA-sIgE水平均高于A、B组和C组 (P 均< 0.05); D组小鼠血清IL-10、TGF-β水平高于C组 (P 均< 0.05)。镜下观察显示, C组小鼠鼻黏膜纤毛脱落缺损明显, 杯状细胞数量增多且体积增大, 间质水肿, 黏膜下见小血管扩张; D组小鼠鼻黏膜病变较C组轻。结论 HCFP对OVA诱导的小鼠AR具有保护效果。." @default.
• W4280543669 created "2022-05-22" @default.
• W4280543669 creator A5000661277 @default.
• W4280543669 creator A5016446062 @default.
• W4280543669 creator A5017189521 @default.
• W4280543669 creator A5017805602 @default.
• W4280543669 creator A5030560508 @default.
• W4280543669 creator A5042140925 @default.
• W4280543669 creator A5042560699 @default.
• W4280543669 creator A5046676165 @default.
• W4280543669 creator A5050103303 @default.
• W4280543669 creator A5064777080 @default.
• W4280543669 creator A5070016516 @default.
• W4280543669 date "2022-02-25" @default.
• W4280543669 modified "2023-09-27" @default.
• W4280543669 title "[Effect of Echinococcus granulosus hydatid cyst fluid protein on allergic rhinitis induced by ovalbumin in mice]." @default.
• W4280543669 doi "https://doi.org/10.16250/j.32.1374.2021276" @default.
• W4280543669 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35537837" @default.
• W4280543669 hasPublicationYear "2022" @default.
• W4280543669 type Work @default.
• W4280543669 citedByCount "0" @default.
• W4280543669 crossrefType "journal-article" @default.
• W4280543669 hasAuthorship W4280543669A5000661277 @default.
• W4280543669 hasAuthorship W4280543669A5016446062 @default.
• W4280543669 hasAuthorship W4280543669A5017189521 @default.
• W4280543669 hasAuthorship W4280543669A5017805602 @default.
• W4280543669 hasAuthorship W4280543669A5030560508 @default.
• W4280543669 hasAuthorship W4280543669A5042140925 @default.
• W4280543669 hasAuthorship W4280543669A5042560699 @default.
• W4280543669 hasAuthorship W4280543669A5046676165 @default.
• W4280543669 hasAuthorship W4280543669A5050103303 @default.
• W4280543669 hasAuthorship W4280543669A5064777080 @default.
• W4280543669 hasAuthorship W4280543669A5070016516 @default.
• W4280543669 hasConcept C125473707 @default.
• W4280543669 hasConcept C134018914 @default.
• W4280543669 hasConcept C141105273 @default.
• W4280543669 hasConcept C142724271 @default.
• W4280543669 hasConcept C153911025 @default.
• W4280543669 hasConcept C159654299 @default.
• W4280543669 hasConcept C165901193 @default.
• W4280543669 hasConcept C16685009 @default.
• W4280543669 hasConcept C185592680 @default.
• W4280543669 hasConcept C191732599 @default.
• W4280543669 hasConcept C203014093 @default.
• W4280543669 hasConcept C2776946954 @default.
• W4280543669 hasConcept C2777028278 @default.
• W4280543669 hasConcept C2777397205 @default.
• W4280543669 hasConcept C2778690821 @default.
• W4280543669 hasConcept C2778718577 @default.
• W4280543669 hasConcept C2910083286 @default.
• W4280543669 hasConcept C71924100 @default.
• W4280543669 hasConcept C74172505 @default.
• W4280543669 hasConcept C74864618 @default.
• W4280543669 hasConcept C86803240 @default.
• W4280543669 hasConcept C8891405 @default.
• W4280543669 hasConcept C89423630 @default.
• W4280543669 hasConcept C90856448 @default.
• W4280543669 hasConceptScore W4280543669C125473707 @default.
• W4280543669 hasConceptScore W4280543669C134018914 @default.
• W4280543669 hasConceptScore W4280543669C141105273 @default.
• W4280543669 hasConceptScore W4280543669C142724271 @default.
• W4280543669 hasConceptScore W4280543669C153911025 @default.
• W4280543669 hasConceptScore W4280543669C159654299 @default.
• W4280543669 hasConceptScore W4280543669C165901193 @default.
• W4280543669 hasConceptScore W4280543669C16685009 @default.
• W4280543669 hasConceptScore W4280543669C185592680 @default.
• W4280543669 hasConceptScore W4280543669C191732599 @default.
• W4280543669 hasConceptScore W4280543669C203014093 @default.
• W4280543669 hasConceptScore W4280543669C2776946954 @default.
• W4280543669 hasConceptScore W4280543669C2777028278 @default.
• W4280543669 hasConceptScore W4280543669C2777397205 @default.
• W4280543669 hasConceptScore W4280543669C2778690821 @default.
• W4280543669 hasConceptScore W4280543669C2778718577 @default.
• W4280543669 hasConceptScore W4280543669C2910083286 @default.
• W4280543669 hasConceptScore W4280543669C71924100 @default.
• W4280543669 hasConceptScore W4280543669C74172505 @default.
• W4280543669 hasConceptScore W4280543669C74864618 @default.
• W4280543669 hasConceptScore W4280543669C86803240 @default.
• W4280543669 hasConceptScore W4280543669C8891405 @default.
• W4280543669 hasConceptScore W4280543669C89423630 @default.
• W4280543669 hasConceptScore W4280543669C90856448 @default.
• W4280543669 hasIssue "2" @default.
• W4280543669 hasLocation W42805436691 @default.
• W4280543669 hasOpenAccess W4280543669 @default.
• W4280543669 hasPrimaryLocation W42805436691 @default.
• W4280543669 hasRelatedWork W1028833 @default.
• W4280543669 hasRelatedWork W1226744 @default.
• W4280543669 hasRelatedWork W1822178 @default.
• W4280543669 hasRelatedWork W19297204 @default.
• W4280543669 hasRelatedWork W4353681 @default.
• W4280543669 hasRelatedWork W4596591 @default.
• W4280543669 hasRelatedWork W4957592 @default.
• W4280543669 hasRelatedWork W5233333 @default.
• W4280543669 hasRelatedWork W577691 @default.
• W4280543669 hasRelatedWork W3101893 @default.
• W4280543669 hasVolume "34" @default.
• W4280543669 isParatext "false" @default.
• W4280543669 isRetracted "false" @default.

• next