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- W4280550264 abstract "Field emission scanning electron microscopy (FESEM) is an essential tool for observing surface details of specimens in a high vacuum. A series of specimen procedures precludes the observations of living organisms, resulting in artifacts. To overcome these problems, Takahiko Hariyama and his colleagues proposed the concept of the nanosuit later referred to as NanoSuit, describing a thin polymer layer placed on organisms to protect them in a high vacuum in 2013. The NanoSuit is formed rapidly by (i) electron beam irradiation, (ii) plasma irradiation, (iii) Tween 20 solution immersion, and (iv) surface shield enhancer (SSE) solution immersion. Without chemical fixation and metal coating, the NanoSuit-formed specimens allowed structural preservation and accurate element detection of insulating, wet specimens at high spatial resolution. NanoSuit-formed larvae were able to resume normal growth following FESEM observation. The method has been employed to observe unfixed and uncoated bacteria, multicellular organisms, and paraffin sections. These results suggest that the NanoSuit can be applied to prolong life in vacuo and overcome the limit of dead imaging of electron microscopy." @default.
- W4280550264 created "2022-05-22" @default.
- W4280550264 creator A5044063776 @default.
- W4280550264 date "2022-05-11" @default.
- W4280550264 modified "2023-09-25" @default.
- W4280550264 title "Biological applications of the NanoSuit for electron imaging and X-microanalysis of insulating specimens" @default.
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- W4280550264 doi "https://doi.org/10.1186/s42649-022-00073-2" @default.
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