Matches in SemOpenAlex for { <https://semopenalex.org/work/W4280551475> ?p ?o ?g. }
- W4280551475 abstract "The reverse genetics system is a valuable tool in the virological study of RNA viruses. With the availability of reverse genetics, the porcine reproductive and respiratory syndrome virus (PRRSV) has been utilized as a viral vector for the expression of foreign genes of interest. Here, we constructed a full-length cDNA clone of a highly pathogenic PRRSV (HP-PRRSV) TA-12 strain. Using this cDNA clone, we generated a reporter virus expressing a gaussia luciferase (Gluc) via an additional subgenomic RNA between ORF7 and 3'UTR. This reporter virus exhibited similar growth kinetics to the wild-type (WT) virus and remained genetically stable for at least ten passages in MARC-145 cells. In cells infected with this reporter virus, the correlation between the expression levels of Gluc in culture media and the virus titers suggested that Gluc is a good indicator of the reporter virus infection. With this reporter virus, we further established the Gluc readout-based assays for antiviral drug screening and serum neutralizing antibody detection that exhibited comparable performance to the classical assays. Taken together, we established a reverse genetics system of HP-PRRSV and generated a novel reporter virus that could serve as a valuable tool for antiviral drug screening and serum neutralizing antibody detection." @default.
- W4280551475 created "2022-05-22" @default.
- W4280551475 creator A5002194150 @default.
- W4280551475 creator A5015263816 @default.
- W4280551475 creator A5020493823 @default.
- W4280551475 creator A5064506268 @default.
- W4280551475 creator A5076626946 @default.
- W4280551475 date "2022-05-13" @default.
- W4280551475 modified "2023-10-09" @default.
- W4280551475 title "A Recombinant Porcine Reproductive and Respiratory Syndrome Virus Stably Expressing a Gaussia Luciferase for Antiviral Drug Screening Assay and Luciferase-Based Neutralization Assay" @default.
- W4280551475 cites W1591311438 @default.
- W4280551475 cites W1769091578 @default.
- W4280551475 cites W1986289228 @default.
- W4280551475 cites W1991776895 @default.
- W4280551475 cites W2000622589 @default.
- W4280551475 cites W2013537667 @default.
- W4280551475 cites W2016452561 @default.
- W4280551475 cites W2036627785 @default.
- W4280551475 cites W2040654802 @default.
- W4280551475 cites W2067429898 @default.
- W4280551475 cites W2074840381 @default.
- W4280551475 cites W2103692755 @default.
- W4280551475 cites W2116652771 @default.
- W4280551475 cites W2138124339 @default.
- W4280551475 cites W2144485355 @default.
- W4280551475 cites W2150786094 @default.
- W4280551475 cites W2158592782 @default.
- W4280551475 cites W2168254025 @default.
- W4280551475 cites W2170759574 @default.
- W4280551475 cites W2180399763 @default.
- W4280551475 cites W2298968286 @default.
- W4280551475 cites W2340951272 @default.
- W4280551475 cites W2412714253 @default.
- W4280551475 cites W2434922242 @default.
- W4280551475 cites W2528935564 @default.
- W4280551475 cites W2621189469 @default.
- W4280551475 cites W2766897332 @default.
- W4280551475 cites W2801721796 @default.
- W4280551475 cites W2809937333 @default.
- W4280551475 cites W2939822128 @default.
- W4280551475 cites W2964231857 @default.
- W4280551475 cites W3010673910 @default.
- W4280551475 cites W3030015573 @default.
- W4280551475 cites W3047205218 @default.
- W4280551475 cites W3096007880 @default.
- W4280551475 cites W3103886971 @default.
- W4280551475 cites W3138337704 @default.
- W4280551475 cites W3191436913 @default.
- W4280551475 cites W3191455212 @default.
- W4280551475 cites W4211060346 @default.
- W4280551475 cites W4212809801 @default.
- W4280551475 cites W4238945854 @default.
- W4280551475 doi "https://doi.org/10.3389/fmicb.2022.907281" @default.
- W4280551475 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35633700" @default.
- W4280551475 hasPublicationYear "2022" @default.
- W4280551475 type Work @default.
- W4280551475 citedByCount "5" @default.
- W4280551475 countsByYear W42805514752022 @default.
- W4280551475 countsByYear W42805514752023 @default.
- W4280551475 crossrefType "journal-article" @default.
- W4280551475 hasAuthorship W4280551475A5002194150 @default.
- W4280551475 hasAuthorship W4280551475A5015263816 @default.
- W4280551475 hasAuthorship W4280551475A5020493823 @default.
- W4280551475 hasAuthorship W4280551475A5064506268 @default.
- W4280551475 hasAuthorship W4280551475A5076626946 @default.
- W4280551475 hasBestOaLocation W42805514751 @default.
- W4280551475 hasConcept C104317684 @default.
- W4280551475 hasConcept C111335760 @default.
- W4280551475 hasConcept C142724271 @default.
- W4280551475 hasConcept C143065580 @default.
- W4280551475 hasConcept C150194340 @default.
- W4280551475 hasConcept C153911025 @default.
- W4280551475 hasConcept C159047783 @default.
- W4280551475 hasConcept C161733203 @default.
- W4280551475 hasConcept C187882448 @default.
- W4280551475 hasConcept C2522874641 @default.
- W4280551475 hasConcept C25365240 @default.
- W4280551475 hasConcept C2776230035 @default.
- W4280551475 hasConcept C2779134260 @default.
- W4280551475 hasConcept C2779656172 @default.
- W4280551475 hasConcept C2779756493 @default.
- W4280551475 hasConcept C2779819720 @default.
- W4280551475 hasConcept C3008058167 @default.
- W4280551475 hasConcept C32611913 @default.
- W4280551475 hasConcept C40767141 @default.
- W4280551475 hasConcept C524204448 @default.
- W4280551475 hasConcept C54009773 @default.
- W4280551475 hasConcept C54355233 @default.
- W4280551475 hasConcept C71924100 @default.
- W4280551475 hasConcept C81089528 @default.
- W4280551475 hasConcept C81885089 @default.
- W4280551475 hasConcept C86803240 @default.
- W4280551475 hasConceptScore W4280551475C104317684 @default.
- W4280551475 hasConceptScore W4280551475C111335760 @default.
- W4280551475 hasConceptScore W4280551475C142724271 @default.
- W4280551475 hasConceptScore W4280551475C143065580 @default.
- W4280551475 hasConceptScore W4280551475C150194340 @default.
- W4280551475 hasConceptScore W4280551475C153911025 @default.
- W4280551475 hasConceptScore W4280551475C159047783 @default.
- W4280551475 hasConceptScore W4280551475C161733203 @default.