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• W4280561623 abstract "Autophagy is important for maintaining homeostasis, nutrient availability and muscle mass in fish. The effect of chloroquine (CQ) and deoxynivalenol (DON) on autophagy-related gene (Atg) regulation was examined in rainbow trout fed with either CQ or DON and compared with pair-fed (nutrient restriction [NR]) and optimally-fed group. Rainbow trout were divided into four groups and, respectively, fed optimal diet (control), diet-containing CQ (6 mg/g), diet-containing DON (0.005 mg/g) or pair-fed the control diet. On days 0, 1, 3, 7, 14 and 21, liver and muscle were sampled to evaluate the expression of atg4, atg5, atg7, atg12, atg13, atg16, bec-1, gabarap and lc3. In both liver and muscle, CQ induced the highest number of Atgs, with eight in liver and seven in muscle; DON-induced six Atgs in liver and three in muscle; and pair-fed induced three Atgs in liver and two in muscle. In vitro, the effect of NR and CQ on Atg was examined in the RTL-W1 cell line. NR induced all Atg in RTL-W1, with the exception of bec-1. Furthermore, NR combined with CQ enhanced Atg expression greater than NR alone. Overall, CQ and DON can modulate Atg in rainbow trout, which can influence physiology and growth in aquaculture." @default.
• W4280561623 created "2022-05-22" @default.
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• W4280561623 date "2022-05-15" @default.
• W4280561623 modified "2023-10-17" @default.
• W4280561623 title "Autophagy‐related gene regulation in liver and muscle of rainbow trout ( <i>Oncorhynchus mykiss</i> ) upon exposure to chloroquine, deoxynivalenol and nutrient restriction" @default.
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• W4280561623 doi "https://doi.org/10.1111/are.15896" @default.
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