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• W4280562135 abstract "There is an increasing need for label free methods that could reveal intracellular structures and dynamics. In this context, we develop a new optical tomography method working in transmission - full-field optical transmission tomography (FF-OTT). The method can measure the forward scattering signals and reveals the time-dependent metabolic signals in living cells. FF-OTT is a common path interferometer taking advantage of the Gouy phase shift - a π phase shift that the light wave experiences around the focus. By modulating the position of the focus one can alter the phase of the scattered light. Demodulation of images with different phases rejects the background and enhances the light from the depth-of-field, thus producing an optical section. We test FF-OTT by imaging single-cell diatoms and ex vivo biological samples. In fresh samples, we show that the intracellular motions create visible intensity fluctuations in FF-OTT so that the method is able to reveal a metabolic dynamic contrast. FF-OTT was found to be an efficient label free technique that can be readily implemented thanks to a robust common-path speckle-free interferometer design using an incoherent light source." @default.
• W4280562135 created "2022-05-22" @default.
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• W4280562135 date "2022-07-08" @default.
• W4280562135 modified "2023-10-09" @default.
• W4280562135 title "Label free optical transmission tomography for biosystems: intracellular structures and dynamics" @default.
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• W4280562135 doi "https://doi.org/10.1364/boe.453586" @default.
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