FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4280567081> ?p ?o ?g. }

• W4280567081 abstract "A previous study reported that the Mycobacterium smegmatis (Msm) protein MSMEG_2295 is a repressor controlling the expression of several genes, including that for MSMEG_5125, a putative isoprenoid binding protein belonging to the YceI family, and DinB2, a DNA damage repair enzyme. This repressor is encoded by the first gene of the operon that also expresses the gene for DinB2. Targeted inhibition of MSMEG_5125 using CRISPRi technology resulted in a significant loss of Msm's respiratory activity and viability. Since this protein has been predicted to be an isoprenoid binding protein, we suspected a role of menaquinones, which are isoprenoid naphthoquinones, in the observed phenomenon. Accordingly, we tested whether MSMEG_5125's deficiency-induced lethality could be reversed by adding menaquinone. The result was positive, implying cooperation between MSMEG_5125 and menaquinone in bringing about respiration. Inhibition of MSMEG_5125 expression led to the induction of MSMEG_0089 and 2296, two hallmark genes of the MSMEG_2295 regulon. This result suggests that when MSMEG_5125 becomes limiting, a feedback-loop derepresses the MSMEG_2295 regulon genes, including its own. Interestingly, menaquinone functioned as an inducer of MSMEG_5125, indicating that it is likely to mediate the feedback mechanism. This result also strengthens our hypothesis that the functions of menaquinone and MSMEG_5125 are interrelated. Menaquinone also induced the MSMEG_2295-controlled operon MSMEG_2295-2294 (dinB2) not induced following the inactivation of MSMEG_5125. Therefore, the activation mechanism of MSMEG_2295-regulated genes may not be the same for all, although derepression is likely to be a common feature. In vitro, menaquinone abolished MSMEG_2295's DNA binding activity by interacting with it, confirming its role as an inducer. Therefore, a menaquinone-MSMEG_5125-regulated gene expression circuit controls Msm respiration and possibly oxidative stress-induced DNA damage repair." @default.
• W4280567081 created "2022-05-22" @default.
• W4280567081 creator A5011625156 @default.
• W4280567081 creator A5026445741 @default.
• W4280567081 creator A5039806644 @default.
• W4280567081 date "2022-05-16" @default.
• W4280567081 modified "2023-09-25" @default.
• W4280567081 title "The respiratory lipoquinone, menaquinone, functions as an inducer of genes regulated by the Mycobacterium smegmatis repressor MSMEG_2295" @default.
• W4280567081 cites W1795451287 @default.
• W4280567081 cites W1969870555 @default.
• W4280567081 cites W1973789730 @default.
• W4280567081 cites W1973838773 @default.
• W4280567081 cites W1981552607 @default.
• W4280567081 cites W1994145955 @default.
• W4280567081 cites W2001454891 @default.
• W4280567081 cites W2004361822 @default.
• W4280567081 cites W2010179561 @default.
• W4280567081 cites W2010483150 @default.
• W4280567081 cites W2041298534 @default.
• W4280567081 cites W2045825103 @default.
• W4280567081 cites W2076946771 @default.
• W4280567081 cites W2082973247 @default.
• W4280567081 cites W2091300474 @default.
• W4280567081 cites W2112997201 @default.
• W4280567081 cites W2129162353 @default.
• W4280567081 cites W2139902189 @default.
• W4280567081 cites W2141637940 @default.
• W4280567081 cites W2146242850 @default.
• W4280567081 cites W2152545013 @default.
• W4280567081 cites W2257065207 @default.
• W4280567081 cites W2282660859 @default.
• W4280567081 cites W2489915703 @default.
• W4280567081 cites W2884269787 @default.
• W4280567081 cites W2990783874 @default.
• W4280567081 cites W3004886518 @default.
• W4280567081 cites W3045580769 @default.
• W4280567081 cites W3158791305 @default.
• W4280567081 cites W3206912254 @default.
• W4280567081 cites W4220743183 @default.
• W4280567081 doi "https://doi.org/10.1099/mic.0.001192" @default.
• W4280567081 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35575764" @default.
• W4280567081 hasPublicationYear "2022" @default.
• W4280567081 type Work @default.
• W4280567081 citedByCount "2" @default.
• W4280567081 countsByYear W42805670812022 @default.
• W4280567081 countsByYear W42805670812023 @default.
• W4280567081 crossrefType "journal-article" @default.
• W4280567081 hasAuthorship W4280567081A5011625156 @default.
• W4280567081 hasAuthorship W4280567081A5026445741 @default.
• W4280567081 hasAuthorship W4280567081A5039806644 @default.
• W4280567081 hasBestOaLocation W42805670811 @default.
• W4280567081 hasConcept C104317684 @default.
• W4280567081 hasConcept C142724271 @default.
• W4280567081 hasConcept C143065580 @default.
• W4280567081 hasConcept C150194340 @default.
• W4280567081 hasConcept C158448853 @default.
• W4280567081 hasConcept C165864922 @default.
• W4280567081 hasConcept C203075996 @default.
• W4280567081 hasConcept C2777204207 @default.
• W4280567081 hasConcept C2777975735 @default.
• W4280567081 hasConcept C2781069245 @default.
• W4280567081 hasConcept C54355233 @default.
• W4280567081 hasConcept C62361671 @default.
• W4280567081 hasConcept C71924100 @default.
• W4280567081 hasConcept C86803240 @default.
• W4280567081 hasConceptScore W4280567081C104317684 @default.
• W4280567081 hasConceptScore W4280567081C142724271 @default.
• W4280567081 hasConceptScore W4280567081C143065580 @default.
• W4280567081 hasConceptScore W4280567081C150194340 @default.
• W4280567081 hasConceptScore W4280567081C158448853 @default.
• W4280567081 hasConceptScore W4280567081C165864922 @default.
• W4280567081 hasConceptScore W4280567081C203075996 @default.
• W4280567081 hasConceptScore W4280567081C2777204207 @default.
• W4280567081 hasConceptScore W4280567081C2777975735 @default.
• W4280567081 hasConceptScore W4280567081C2781069245 @default.
• W4280567081 hasConceptScore W4280567081C54355233 @default.
• W4280567081 hasConceptScore W4280567081C62361671 @default.
• W4280567081 hasConceptScore W4280567081C71924100 @default.
• W4280567081 hasConceptScore W4280567081C86803240 @default.
• W4280567081 hasIssue "5" @default.
• W4280567081 hasLocation W42805670811 @default.
• W4280567081 hasLocation W42805670812 @default.
• W4280567081 hasOpenAccess W4280567081 @default.
• W4280567081 hasPrimaryLocation W42805670811 @default.
• W4280567081 hasRelatedWork W1855310881 @default.
• W4280567081 hasRelatedWork W2095458827 @default.
• W4280567081 hasRelatedWork W2125904490 @default.
• W4280567081 hasRelatedWork W2141677631 @default.
• W4280567081 hasRelatedWork W2171972144 @default.
• W4280567081 hasRelatedWork W2497148628 @default.
• W4280567081 hasRelatedWork W2807652062 @default.
• W4280567081 hasRelatedWork W2997496721 @default.
• W4280567081 hasRelatedWork W4213084828 @default.
• W4280567081 hasRelatedWork W4280567081 @default.
• W4280567081 hasVolume "168" @default.
• W4280567081 isParatext "false" @default.
• W4280567081 isRetracted "false" @default.
• W4280567081 workType "article" @default.