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- W4280567572 abstract "The detection of small molecules interacting with DNA is important for the assessment of potential hazards related to the application of rather toxic antitumor drugs, and for distinguishing the factors related to thermal and oxidative DNA damage. In this work, a novel electrochemical DNA sensor has been proposed for the determination of antitumor drugs. For DNA sensor assembling, a glassy carbon electrode was modified with carbon black dispersed in DMF. After that, pillar [5]arene was adsorbed and Methylene blue and Neutral red were consecutively electropolymerized onto the carbon black layer. To increase sensitivity of intercalator detection, DNA was first mixed with water-soluble thiacalixarene bearing quaternary ammonium groups in the substituents at the lower rim. The deposition of the mixture on the electropolymerized dyes made it possible to detect doxorubicin as model intercalator by suppression of the redox activity of the polymerization products. The DNA sensor made it possible to determine 0.5 pM–1.0 nM doxorubicin (limit of detection 0.13 pM) with 20 min of incubation. The DNA sensor was successfully tested on spiked samples of human plasma and doxorubicin medication." @default.
- W4280567572 created "2022-05-22" @default.
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- W4280567572 date "2022-05-12" @default.
- W4280567572 modified "2023-10-15" @default.
- W4280567572 title "Electrochemical DNA Sensor Based on Carbon Black—Poly(Methylene Blue)—Poly(Neutral Red) Composite" @default.
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- W4280567572 doi "https://doi.org/10.3390/bios12050329" @default.
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