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- W4280581848 abstract "Abstract Mitochondria are complex organelles that play a central role in metabolism. Dynamic membrane-associated processes regulate mitochondrial morphology and bioenergetics in response to cellular demand. In tumor cells, metabolic reprogramming requires active mitochondrial metabolism for providing key metabolites and building blocks for tumor growth and rapid proliferation. To counter this, the mitochondrial serine beta-lactamase-like protein (LACTB) alters mitochondrial lipid metabolism and potently inhibits the proliferation of a variety of tumor cells. Mammalian LACTB is localized in the mitochondrial intermembrane space, where it assembles into filaments to regulate the efficiency of essential metabolic processes. However, the structural basis of LACTB polymerization and regulation remains incompletely understood. Here, we describe how human LACTB self-assembles into micron-scale filaments that increase their catalytic activity. The electron cryo-microscopy (cryoEM) structure defines the mechanism of assembly and reveals how highly ordered filament bundles stabilize the active state of the enzyme. We identify and characterize residues that are located at the filament-forming interface, and further show that mutations that disrupt filamentation reduce enzyme activity. Furthermore, our results provide evidence that LACTB filaments can bind lipid membranes. These data reveal the detailed molecular organization and polymerization-based regulation of human LACTB and provide new insights into the mechanism of mitochondrial membrane organization that modulates lipid metabolism." @default.
- W4280581848 created "2022-05-22" @default.
- W4280581848 creator A5006843474 @default.
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- W4280581848 creator A5029228941 @default.
- W4280581848 creator A5044766909 @default.
- W4280581848 creator A5091863599 @default.
- W4280581848 date "2022-04-22" @default.
- W4280581848 modified "2023-09-26" @default.
- W4280581848 title "The structure of the human LACTB filament reveals the mechanisms of assembly and membrane binding" @default.
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- W4280581848 doi "https://doi.org/10.1101/2022.04.21.489104" @default.
- W4280581848 hasPublicationYear "2022" @default.
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