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- W4280587769 abstract "Abstract Emerging evidence points towards a regulatory role of the circadian clock in alternative splicing (AS). Whether alterations in core-clock components may contribute to differential AS events is largely unknown. To address this, we carried out a computational analysis on recently generated time-series RNA-seq datasets from three core-clock knockout (KO) genes ( ARNTL , NR1D1 , PER2 ) and WT of a colorectal cancer (CRC) cell line, and time-series RNA-seq datasets for additional CRC and Hodgkin’s lymphoma (HL) cells, murine WT, Arntl KO, and Nr1d1/2 KO, and murine SCN WT tissue. The deletion of individual core-clock genes resulted in the loss of circadian expression in crucial spliceosome components such as SF3A1 (in ARNTL KO ), SNW1 (in NR1D1 KO ), and HNRNPC (in PER2 KO ), which led to a differential pattern of KO-specific AS events. All HCT116 KO cells showed a rhythmicity loss of a crucial spliceosome gene U2AF1 , which was also not rhythmic in higher progression stage CRC and HL cancer cells. AS analysis revealed an increase in alternative first exon events specific to PER2 and NR1D1 KO in HCT116 cells, and a KO-specific change in expression and rhythmicity pattern of AS transcripts related to cancer hallmarks genes including FGFR2 in HCT116_ ARNTL KO , CD44 in HCT116_ NR1D1 KO , and MET in HCT116_ PER2 KO . KO-specific changes in rhythmic properties of known spliced variants of these genes (e.g. FGFR2 IIIb/ FGFR2 IIIc) correlated with epithelial-mesenchymal-transition signalling. Altogether, our bioinformatic analysis highlights a role for the circadian clock in the regulation of AS, and reveals a potential impact of clock disruption in aberrant splicing in cancer hallmark genes." @default.
- W4280587769 created "2022-05-22" @default.
- W4280587769 creator A5041763620 @default.
- W4280587769 creator A5050627192 @default.
- W4280587769 creator A5073992730 @default.
- W4280587769 date "2022-05-12" @default.
- W4280587769 modified "2023-10-12" @default.
- W4280587769 title "Transcriptome analysis of clock disrupted cancer cells reveals differential alternative splicing of cancer hallmarks genes" @default.
- W4280587769 cites W1816471535 @default.
- W4280587769 cites W1929552083 @default.
- W4280587769 cites W1965266937 @default.
- W4280587769 cites W1975381672 @default.
- W4280587769 cites W1976221241 @default.
- W4280587769 cites W1983567075 @default.
- W4280587769 cites W1987128123 @default.
- W4280587769 cites W1988528648 @default.
- W4280587769 cites W1993780097 @default.
- W4280587769 cites W2009258895 @default.
- W4280587769 cites W2011248839 @default.
- W4280587769 cites W2020913453 @default.
- W4280587769 cites W2021607553 @default.
- W4280587769 cites W2022495797 @default.
- W4280587769 cites W2024226116 @default.
- W4280587769 cites W2026455198 @default.
- W4280587769 cites W2029039082 @default.
- W4280587769 cites W2035618305 @default.
- W4280587769 cites W2036610074 @default.
- W4280587769 cites W2038684937 @default.
- W4280587769 cites W2040481343 @default.
- W4280587769 cites W2040870350 @default.
- W4280587769 cites W2047941064 @default.
- W4280587769 cites W2052799891 @default.
- W4280587769 cites W2053757573 @default.
- W4280587769 cites W2056315941 @default.
- W4280587769 cites W2061657172 @default.
- W4280587769 cites W2071509829 @default.
- W4280587769 cites W2086859115 @default.
- W4280587769 cites W2087179902 @default.
- W4280587769 cites W2087751835 @default.
- W4280587769 cites W2095242414 @default.
- W4280587769 cites W2096086497 @default.
- W4280587769 cites W2098064548 @default.
- W4280587769 cites W2098342740 @default.
- W4280587769 cites W2104607769 @default.
- W4280587769 cites W2108174762 @default.
- W4280587769 cites W2108585052 @default.
- W4280587769 cites W2114104545 @default.
- W4280587769 cites W2117609446 @default.
- W4280587769 cites W2119169141 @default.
- W4280587769 cites W2119702047 @default.
- W4280587769 cites W2121052112 @default.
- W4280587769 cites W2121847288 @default.
- W4280587769 cites W2122863289 @default.
- W4280587769 cites W2123098388 @default.
- W4280587769 cites W2125292266 @default.
- W4280587769 cites W2126239735 @default.
- W4280587769 cites W2128012941 @default.
- W4280587769 cites W2129029860 @default.
- W4280587769 cites W2131271579 @default.
- W4280587769 cites W2141781797 @default.
- W4280587769 cites W2142257714 @default.
- W4280587769 cites W2143858946 @default.
- W4280587769 cites W2145506897 @default.
- W4280587769 cites W2153833484 @default.
- W4280587769 cites W2155200613 @default.
- W4280587769 cites W2155804510 @default.
- W4280587769 cites W2156003764 @default.
- W4280587769 cites W2156564520 @default.
- W4280587769 cites W2163485494 @default.
- W4280587769 cites W2165781226 @default.
- W4280587769 cites W2165902194 @default.
- W4280587769 cites W2166902893 @default.
- W4280587769 cites W2169456326 @default.
- W4280587769 cites W2170818583 @default.
- W4280587769 cites W2197124664 @default.
- W4280587769 cites W2197459998 @default.
- W4280587769 cites W2318205119 @default.
- W4280587769 cites W2333798941 @default.
- W4280587769 cites W2399067949 @default.
- W4280587769 cites W2401877286 @default.
- W4280587769 cites W2515668871 @default.
- W4280587769 cites W2516438616 @default.
- W4280587769 cites W2524270605 @default.
- W4280587769 cites W2571333200 @default.
- W4280587769 cites W2588101937 @default.
- W4280587769 cites W2592811885 @default.
- W4280587769 cites W2605106690 @default.
- W4280587769 cites W2605134565 @default.
- W4280587769 cites W2605452842 @default.
- W4280587769 cites W2610328326 @default.
- W4280587769 cites W2612976808 @default.
- W4280587769 cites W2615119360 @default.
- W4280587769 cites W2624114007 @default.
- W4280587769 cites W2626464402 @default.
- W4280587769 cites W2750771238 @default.
- W4280587769 cites W2766537551 @default.
- W4280587769 cites W2773908748 @default.
- W4280587769 cites W2795877840 @default.
- W4280587769 cites W2802552316 @default.
- W4280587769 cites W2809312521 @default.