FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4280626614> ?p ?o ?g. }

• W4280626614 abstract "Synthetic oligodeoxynucleotides (ODN) have various applications in many areas such as, synthetic biology, chemical biology, antisense drug development, and data storage. As a result, there is a high demand for synthetic ODNs. Many advances have been made with ODN synthesis the purification still remains a bottleneck. The non-chromatographic purification method is developed to address this problem. Similarly, polyethylene glycols (PEGs) have been used in many areas including pharmaceutical applications, surface science, and nanomedicine due to its unique properties. However, monodispersed PEG synthesis is expensive with existing methods. A novel automated solid phase synthesis method is developed to obtain monodispersed PEGs. The first two chapters are an introduction to synthetic oligodeoxynucleotide purification. These chapters describe oligonucleotides and applications, chemical synthesis of oligodeoxynucleotides, impurities in synthetic oligonucleotides, and current purification methods. Even though many chromatographic purification methods are available for synthetic ODN purification, there is no method that is capable of high throughput purification, large scale ODN purification, and long ODN purification. To address this problem, we developed two non-chromatographic purification methods which are based on polymerization, to purify synthetic ODNs. One is catching failure sequences by polymerization, and the second method is catching full-length sequences by polymerization. The second method is less expensive for large-scale and long ODN purification compared to the first method. It is demonstrated that this new non-chromatographic method is suitable for high throughput, large scale, and long ODN purification. The final chapters describe applications of polyethylene glycol and current PEG synthesis methods. For many applications monodispersed PEGs are preferred. However, PEGs synthesized using current synthesis methods are polydispersed and excessive purification is required to obtain monodispersed PEGs. Therefore, it is expensive to obtain monodispersed PEGs using these existing methods. An automated solid-phase stepwise synthesis method is developed for monodispersed PEG synthesis. This solid-phase xxii synthesis method is cost effective because the purification is not required to obtain monodispersed PEGs using this method. Monodispersed PEGs with eight, twelve, and sixteen ethylene glycol units and their derivatives were synthesized using automated stepwise addition of tetra ethylene glycol monomer without chromatographic purification. The monomer consists of a 4,4′ dimethoxy trityl group at one end and the tosyl group at the other hydroxyl group. Wang resin was used as the solid support for the automated synthesis. The automated synthesis cycle consists of three steps, deprotonation, coupling, and detritylation. PEGs were cleaved off from the solid support and analyzed with ESI-MS. After PEG12 synthesis, the coupling step with DMTr monomer had some complications and therefore a new monomer with a smaller protecting group was designed. Using this new monomer monodispersed PEGs with ten, fifteen, and twenty ethylene glycol units were synthesized. The synthesis was done using automated solid-phase stepwise addition of penta ethylene glycol units without chromatographic purification. The penta ethylene glycol monomer consists of a 2-phenethyl group at one end, and the tosyl group at the other hydroxyl group was used for the synthesis. The first step is deprotection and deprotonation, and the second step is coupling. These two steps were performed alternatively until the PEG with the desired length was obtained. After the synthesis, PEGs were cleaved from the solid support and analyzed using ESI-MS." @default.
• W4280626614 created "2022-05-22" @default.
• W4280626614 creator A5054484200 @default.
• W4280626614 date "2022-05-13" @default.
• W4280626614 modified "2023-10-14" @default.
• W4280626614 title "NON-CHROMATOGRAPHIC OLIGONUCLEOTIDE PURIFICATION AND AUTOMATED POLYETHYLENEGLYCOL SYNTHESIS" @default.
• W4280626614 doi "https://doi.org/10.37099/mtu.dc.etdr/1396" @default.
• W4280626614 hasPublicationYear "2022" @default.
• W4280626614 type Work @default.
• W4280626614 citedByCount "0" @default.
• W4280626614 crossrefType "dissertation" @default.
• W4280626614 hasAuthorship W4280626614A5054484200 @default.
• W4280626614 hasBestOaLocation W42806266141 @default.
• W4280626614 hasConcept C10138342 @default.
• W4280626614 hasConcept C129312508 @default.
• W4280626614 hasConcept C162324750 @default.
• W4280626614 hasConcept C178790620 @default.
• W4280626614 hasConcept C185592680 @default.
• W4280626614 hasConcept C21951064 @default.
• W4280626614 hasConcept C2776964284 @default.
• W4280626614 hasConcept C43617362 @default.
• W4280626614 hasConcept C44228677 @default.
• W4280626614 hasConcept C521977710 @default.
• W4280626614 hasConcept C54400483 @default.
• W4280626614 hasConcept C552990157 @default.
• W4280626614 hasConcept C55493867 @default.
• W4280626614 hasConceptScore W4280626614C10138342 @default.
• W4280626614 hasConceptScore W4280626614C129312508 @default.
• W4280626614 hasConceptScore W4280626614C162324750 @default.
• W4280626614 hasConceptScore W4280626614C178790620 @default.
• W4280626614 hasConceptScore W4280626614C185592680 @default.
• W4280626614 hasConceptScore W4280626614C21951064 @default.
• W4280626614 hasConceptScore W4280626614C2776964284 @default.
• W4280626614 hasConceptScore W4280626614C43617362 @default.
• W4280626614 hasConceptScore W4280626614C44228677 @default.
• W4280626614 hasConceptScore W4280626614C521977710 @default.
• W4280626614 hasConceptScore W4280626614C54400483 @default.
• W4280626614 hasConceptScore W4280626614C552990157 @default.
• W4280626614 hasConceptScore W4280626614C55493867 @default.
• W4280626614 hasLocation W42806266141 @default.
• W4280626614 hasOpenAccess W4280626614 @default.
• W4280626614 hasPrimaryLocation W42806266141 @default.
• W4280626614 hasRelatedWork W1577519637 @default.
• W4280626614 hasRelatedWork W1974434168 @default.
• W4280626614 hasRelatedWork W2011392986 @default.
• W4280626614 hasRelatedWork W2031600117 @default.
• W4280626614 hasRelatedWork W2037664463 @default.
• W4280626614 hasRelatedWork W2044935159 @default.
• W4280626614 hasRelatedWork W2060671635 @default.
• W4280626614 hasRelatedWork W2074277554 @default.
• W4280626614 hasRelatedWork W2087348933 @default.
• W4280626614 hasRelatedWork W2150895058 @default.
• W4280626614 isParatext "false" @default.
• W4280626614 isRetracted "false" @default.
• W4280626614 workType "dissertation" @default.