Matches in SemOpenAlex for { <https://semopenalex.org/work/W4280635963> ?p ?o ?g. }
- W4280635963 abstract "Here, we report the molecular engineering of nanobodies that bind with picomolar affinity to both SARS-CoV-1 and SARS-CoV-2 receptor-binding domains (RBD) and are highly neutralizing. We applied deep mutational engineering to VHH72, a nanobody initially specific for SARS-CoV-1 RBD with little cross-reactivity to SARS-CoV-2 antigen. We first identified all the individual VHH substitutions that increase binding to SARS-CoV-2 RBD and then screened highly focused combinatorial libraries to isolate engineered nanobodies with improved properties. The corresponding VHH-Fc molecules show high affinities for SARS-CoV-2 antigens from various emerging variants and SARS-CoV-1, block the interaction between ACE2 and RBD, and neutralize the virus with high efficiency. Its rare specificity across sarbecovirus relies on its peculiar epitope outside the immunodominant regions. The engineered nanobodies share a common motif of three amino acids, which contribute to the broad specificity of recognition. Our results show that deep mutational engineering is a very powerful method, especially to rapidly adapt existing antibodies to new variants of pathogens." @default.
- W4280635963 created "2022-05-22" @default.
- W4280635963 creator A5001066329 @default.
- W4280635963 creator A5017430420 @default.
- W4280635963 creator A5018444800 @default.
- W4280635963 creator A5023708665 @default.
- W4280635963 creator A5026092895 @default.
- W4280635963 creator A5030715163 @default.
- W4280635963 creator A5034046540 @default.
- W4280635963 creator A5047839691 @default.
- W4280635963 creator A5065042887 @default.
- W4280635963 creator A5066336707 @default.
- W4280635963 creator A5073145975 @default.
- W4280635963 creator A5080973703 @default.
- W4280635963 date "2022-05-20" @default.
- W4280635963 modified "2023-10-16" @default.
- W4280635963 title "Deep mutational engineering of broadly-neutralizing nanobodies accommodating SARS-CoV-1 and 2 antigenic drift" @default.
- W4280635963 cites W1954202239 @default.
- W4280635963 cites W2006648563 @default.
- W4280635963 cites W2024798162 @default.
- W4280635963 cites W2029667189 @default.
- W4280635963 cites W2065283382 @default.
- W4280635963 cites W2067174909 @default.
- W4280635963 cites W2102686641 @default.
- W4280635963 cites W2106140689 @default.
- W4280635963 cites W2107409328 @default.
- W4280635963 cites W2107493728 @default.
- W4280635963 cites W2109154308 @default.
- W4280635963 cites W2132262459 @default.
- W4280635963 cites W2146522960 @default.
- W4280635963 cites W2288202420 @default.
- W4280635963 cites W2526473535 @default.
- W4280635963 cites W2794687643 @default.
- W4280635963 cites W2800784982 @default.
- W4280635963 cites W3017133925 @default.
- W4280635963 cites W3023698680 @default.
- W4280635963 cites W3025553169 @default.
- W4280635963 cites W3025744748 @default.
- W4280635963 cites W3029682964 @default.
- W4280635963 cites W3046751266 @default.
- W4280635963 cites W3046828715 @default.
- W4280635963 cites W3048714202 @default.
- W4280635963 cites W3049166744 @default.
- W4280635963 cites W3093036893 @default.
- W4280635963 cites W3096064145 @default.
- W4280635963 cites W3096658347 @default.
- W4280635963 cites W3096751000 @default.
- W4280635963 cites W3111294048 @default.
- W4280635963 cites W3111431752 @default.
- W4280635963 cites W3119147846 @default.
- W4280635963 cites W3119294059 @default.
- W4280635963 cites W3121833403 @default.
- W4280635963 cites W3121995178 @default.
- W4280635963 cites W3122134057 @default.
- W4280635963 cites W3123708706 @default.
- W4280635963 cites W3127852352 @default.
- W4280635963 cites W3133782701 @default.
- W4280635963 cites W3133980772 @default.
- W4280635963 cites W3144378969 @default.
- W4280635963 cites W3149428818 @default.
- W4280635963 cites W3165032628 @default.
- W4280635963 cites W3173700036 @default.
- W4280635963 cites W3178509854 @default.
- W4280635963 cites W3179071715 @default.
- W4280635963 cites W3179259355 @default.
- W4280635963 cites W3182094313 @default.
- W4280635963 cites W3187486466 @default.
- W4280635963 cites W3192451245 @default.
- W4280635963 cites W3202475074 @default.
- W4280635963 cites W3202951415 @default.
- W4280635963 cites W4220664012 @default.
- W4280635963 cites W4221072241 @default.
- W4280635963 doi "https://doi.org/10.1080/19420862.2022.2076775" @default.
- W4280635963 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35593235" @default.
- W4280635963 hasPublicationYear "2022" @default.
- W4280635963 type Work @default.
- W4280635963 citedByCount "4" @default.
- W4280635963 countsByYear W42806359632022 @default.
- W4280635963 countsByYear W42806359632023 @default.
- W4280635963 crossrefType "journal-article" @default.
- W4280635963 hasAuthorship W4280635963A5001066329 @default.
- W4280635963 hasAuthorship W4280635963A5017430420 @default.
- W4280635963 hasAuthorship W4280635963A5018444800 @default.
- W4280635963 hasAuthorship W4280635963A5023708665 @default.
- W4280635963 hasAuthorship W4280635963A5026092895 @default.
- W4280635963 hasAuthorship W4280635963A5030715163 @default.
- W4280635963 hasAuthorship W4280635963A5034046540 @default.
- W4280635963 hasAuthorship W4280635963A5047839691 @default.
- W4280635963 hasAuthorship W4280635963A5065042887 @default.
- W4280635963 hasAuthorship W4280635963A5066336707 @default.
- W4280635963 hasAuthorship W4280635963A5073145975 @default.
- W4280635963 hasAuthorship W4280635963A5080973703 @default.
- W4280635963 hasBestOaLocation W42806359631 @default.
- W4280635963 hasConcept C104317684 @default.
- W4280635963 hasConcept C142724271 @default.
- W4280635963 hasConcept C147483822 @default.
- W4280635963 hasConcept C147816474 @default.
- W4280635963 hasConcept C159047783 @default.
- W4280635963 hasConcept C159654299 @default.
- W4280635963 hasConcept C167625842 @default.