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- W4281286407 abstract "Protozoa play important roles in microbial communities, regulating populations via predation and contributing to nutrient cycling. While amoebae have been identified in acid rock drainage (ARD) systems, our understanding of their symbioses in these extreme environments is limited. Here, we report the first isolation of the amoeba Stemonitis from an ARD environment as well as the genome sequence and annotation of an associated bacterium, Dyella terrae strain Ely Copper Mine, from Ely Brook at the Ely Copper Mine Superfund site in Vershire, Vermont, United States. Fluorescent in situ hybridization analysis showed this bacterium colonizing cells of Stemonitis sp. in addition to being outside of amoebal cells. This amoeba-resistant bacterium is Gram-negative with a genome size of 5.36 Mbp and GC content of 62.5%. The genome of the D. terrae strain Ely Copper Mine encodes de novo biosynthetic pathways for amino acids, carbohydrates, nucleic acids, and lipids. Genes involved in nitrate (1) and sulfate (7) reduction, metal (229) and antibiotic resistance (37), and secondary metabolite production (6) were identified. Notably, 26 hydrolases were identified by RAST as well as other biomass degradation genes, suggesting roles in carbon and energy cycling within the microbial community. The genome also contains type IV secretion system genes involved in amoebae resistance, revealing how this bacterium likely survives predation from Stemonitis sp. This genome analysis and the association of D. terrae strain Ely Copper Mine with Stemonitis sp. provide insight into the functional roles of amoebae and bacteria within ARD environments." @default.
- W4281286407 created "2022-05-24" @default.
- W4281286407 creator A5005921613 @default.
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- W4281286407 creator A5083240202 @default.
- W4281286407 date "2022-05-23" @default.
- W4281286407 modified "2023-10-14" @default.
- W4281286407 title "Isolation and Genome Analysis of an Amoeba-Associated Bacterium Dyella terrae Strain Ely Copper Mine From Acid Rock Drainage in Vermont, United States" @default.
- W4281286407 cites W1600572471 @default.
- W4281286407 cites W1842614524 @default.
- W4281286407 cites W1970216705 @default.
- W4281286407 cites W1970312993 @default.
- W4281286407 cites W1981860166 @default.
- W4281286407 cites W1990989901 @default.
- W4281286407 cites W2004548026 @default.
- W4281286407 cites W2004589024 @default.
- W4281286407 cites W2008835132 @default.
- W4281286407 cites W2013038758 @default.
- W4281286407 cites W2016866428 @default.
- W4281286407 cites W2027184506 @default.
- W4281286407 cites W2031003438 @default.
- W4281286407 cites W2034495836 @default.
- W4281286407 cites W2045204781 @default.
- W4281286407 cites W2055043387 @default.
- W4281286407 cites W2065506174 @default.
- W4281286407 cites W2074898106 @default.
- W4281286407 cites W2075158754 @default.
- W4281286407 cites W2081730917 @default.
- W4281286407 cites W2087531418 @default.
- W4281286407 cites W2092604303 @default.
- W4281286407 cites W2096093282 @default.
- W4281286407 cites W2097812334 @default.
- W4281286407 cites W2099571549 @default.
- W4281286407 cites W2112362775 @default.
- W4281286407 cites W2112364185 @default.
- W4281286407 cites W2116226613 @default.
- W4281286407 cites W2128776472 @default.
- W4281286407 cites W2130122306 @default.
- W4281286407 cites W2131227464 @default.
- W4281286407 cites W2132926880 @default.
- W4281286407 cites W2136397207 @default.
- W4281286407 cites W2137624002 @default.
- W4281286407 cites W2144783616 @default.
- W4281286407 cites W2152866254 @default.
- W4281286407 cites W2154775341 @default.
- W4281286407 cites W2159482845 @default.
- W4281286407 cites W2170551349 @default.
- W4281286407 cites W2279118269 @default.
- W4281286407 cites W2328622427 @default.
- W4281286407 cites W2466635549 @default.
- W4281286407 cites W2469730249 @default.
- W4281286407 cites W2512643090 @default.
- W4281286407 cites W2554447269 @default.
- W4281286407 cites W2559099718 @default.
- W4281286407 cites W2579533542 @default.
- W4281286407 cites W2588803875 @default.
- W4281286407 cites W2595861342 @default.
- W4281286407 cites W2605054394 @default.
- W4281286407 cites W2610680882 @default.
- W4281286407 cites W2611912271 @default.
- W4281286407 cites W2614081736 @default.
- W4281286407 cites W2726572965 @default.
- W4281286407 cites W2765848665 @default.
- W4281286407 cites W2767730840 @default.
- W4281286407 cites W2781706726 @default.
- W4281286407 cites W2788228074 @default.
- W4281286407 cites W2793879070 @default.
- W4281286407 cites W2799879581 @default.
- W4281286407 cites W2885761488 @default.
- W4281286407 cites W2900116919 @default.
- W4281286407 cites W2903139441 @default.
- W4281286407 cites W2916238052 @default.
- W4281286407 cites W2919601071 @default.
- W4281286407 cites W2923639277 @default.
- W4281286407 cites W2941771989 @default.
- W4281286407 cites W2943271611 @default.
- W4281286407 cites W2950354111 @default.
- W4281286407 cites W2951912016 @default.
- W4281286407 cites W2969197724 @default.
- W4281286407 cites W3004792267 @default.
- W4281286407 cites W3005235420 @default.
- W4281286407 cites W3021832801 @default.
- W4281286407 cites W3026412036 @default.
- W4281286407 cites W3039837527 @default.
- W4281286407 cites W3045779340 @default.
- W4281286407 cites W3086946683 @default.
- W4281286407 cites W3089868668 @default.
- W4281286407 cites W3091506611 @default.
- W4281286407 cites W3094938478 @default.
- W4281286407 cites W3118544462 @default.
- W4281286407 cites W3161596995 @default.
- W4281286407 cites W3186309583 @default.
- W4281286407 cites W3207301244 @default.
- W4281286407 cites W4210971425 @default.
- W4281286407 cites W4226092504 @default.