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- W4281479319 abstract "MAF1, a key repressor of RNA polymerase (pol) III-mediated transcription, has been shown to promote mesoderm formation in vitro. Here, we show that MAF1 plays a critical role in regulating osteoblast differentiation and bone mass. Global deletion of MAF1 (Maf1-/- mice) produced a high bone mass phenotype. However, osteoblasts isolated from Maf1-/- mice showed reduced osteoblastogenesis ex vivo. Therefore, we determined the phenotype of mice overexpressing MAF1 in cells from the mesenchymal lineage (Prx1-Cre;LSL-MAF1 mice). These mice showed increased bone mass. Ex vivo, cells from these mice showed enhanced osteoblastogenesis concordant with their high bone mass phenotype. Thus, the high bone mass phenotype in Maf1-/- mice is likely due to confounding effects from the global absence of MAF1. MAF1 overexpression promoted osteoblast differentiation of ST2 cells while MAF1 downregulation inhibited differentiation, indicating MAF1 enhances osteoblast formation. However, other perturbations used to repress RNA pol III transcription, inhibited osteoblast differentiation. However, decreasing RNA pol III transcription through these perturbations enhanced adipogenesis in ST2 cells. RNA-seq analyzed the basis for these opposing actions on osteoblast differentiation. The different modalities used to perturb RNA pol III transcription resulted in distinct gene expression changes, indicating that this transcription process is highly sensitive and triggers diverse gene expression programs and phenotypic outcomes. Specifically, MAF1 induced genes known to promote osteoblast differentiation. Furthermore, genes that are induced during osteoblast differentiation displayed codon bias. Together, these results reveal a novel role for MAF1 and RNA pol III-mediated transcription in osteoblast fate determination, differentiation, and bone mass regulation." @default.
- W4281479319 created "2022-05-26" @default.
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- W4281479319 date "2022-05-25" @default.
- W4281479319 modified "2023-10-01" @default.
- W4281479319 title "MAF1, a repressor of RNA polymerase III-dependent transcription, regulates bone mass" @default.
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- W4281479319 cites W1966413612 @default.
- W4281479319 cites W1988541338 @default.
- W4281479319 cites W1997503128 @default.
- W4281479319 cites W1998372028 @default.
- W4281479319 cites W2009424879 @default.
- W4281479319 cites W2009709163 @default.
- W4281479319 cites W2014114389 @default.
- W4281479319 cites W2014793949 @default.
- W4281479319 cites W2036301524 @default.
- W4281479319 cites W2040796616 @default.
- W4281479319 cites W2043848225 @default.
- W4281479319 cites W2045493291 @default.
- W4281479319 cites W2047496722 @default.
- W4281479319 cites W2047998890 @default.
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- W4281479319 cites W2078724541 @default.
- W4281479319 cites W2080757130 @default.
- W4281479319 cites W2087936477 @default.
- W4281479319 cites W2088580832 @default.
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- W4281479319 cites W2152685009 @default.
- W4281479319 cites W2155937527 @default.
- W4281479319 cites W2161340657 @default.
- W4281479319 cites W2269475172 @default.
- W4281479319 cites W2278252047 @default.
- W4281479319 cites W2294067750 @default.
- W4281479319 cites W2295503655 @default.
- W4281479319 cites W2346254348 @default.
- W4281479319 cites W2476085369 @default.
- W4281479319 cites W2499540508 @default.
- W4281479319 cites W2539022061 @default.
- W4281479319 cites W2570305163 @default.
- W4281479319 cites W2598973820 @default.
- W4281479319 cites W2608468149 @default.
- W4281479319 cites W2623695670 @default.
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- W4281479319 cites W2751076435 @default.
- W4281479319 cites W2789293233 @default.
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- W4281479319 cites W2888496837 @default.
- W4281479319 cites W2900286197 @default.
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- W4281479319 cites W2948060095 @default.
- W4281479319 cites W2950800259 @default.
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- W4281479319 cites W2986904455 @default.
- W4281479319 cites W2989880397 @default.
- W4281479319 cites W2995649537 @default.
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- W4281479319 doi "https://doi.org/10.7554/elife.74740" @default.
- W4281479319 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35611941" @default.
- W4281479319 hasPublicationYear "2022" @default.
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