Matches in SemOpenAlex for { <https://semopenalex.org/work/W4281481570> ?p ?o ?g. }
- W4281481570 endingPage "5913" @default.
- W4281481570 startingPage "5913" @default.
- W4281481570 abstract "(1) Background: Systemic infection is associated with increased neuroinflammation and accelerated cognitive decline in AD patients. Activated neutrophils produce neutrophil-derived microvesicles (NMV), which are internalised by human brain microvascular endothelial cells and increase their permeability in vitro, suggesting that NMV play a role in blood-brain barrier (BBB) integrity during infection. The current study investigated whether microRNA content of NMV from AD patients is significantly different compared to healthy controls and could impact cerebrovascular integrity. (2) Methods: Neutrophils isolated from peripheral blood samples of five AD and five healthy control donors without systemic infection were stimulated to produce NMV. MicroRNAs isolated from NMV were analysed by RNA-Seq, and online bioinformatic tools were used to identify significantly differentially expressed microRNAs in the NMV. Target and pathway analyses were performed to predict the impact of the candidate microRNAs on vascular integrity. (3) Results: There was no significant difference in either the number of neutrophils (p = 0.309) or the number of NMV (p = 0.3434) isolated from AD donors compared to control. However, 158 microRNAs were significantly dysregulated in AD NMV compared to controls, some of which were associated with BBB dysfunction, including miR-210, miR-20b-5p and miR-126-5p. Pathway analysis revealed numerous significantly affected pathways involved in regulating vascular integrity, including the TGFβ and PDGFB pathways, as well as Hippo, IL-2 and DNA damage signalling. (4) Conclusions: NMV from AD patients contain miRNAs that may alter the integrity of the BBB and represent a novel neutrophil-mediated mechanism for BBB dysfunction in AD and the accelerated cognitive decline seen as a result of a systemic infection." @default.
- W4281481570 created "2022-05-26" @default.
- W4281481570 creator A5024162300 @default.
- W4281481570 creator A5052152168 @default.
- W4281481570 creator A5057685617 @default.
- W4281481570 creator A5064130685 @default.
- W4281481570 creator A5064934232 @default.
- W4281481570 creator A5072105682 @default.
- W4281481570 creator A5081159423 @default.
- W4281481570 creator A5091901475 @default.
- W4281481570 date "2022-05-25" @default.
- W4281481570 modified "2023-10-18" @default.
- W4281481570 title "RNA-Seq Profiling of Neutrophil-Derived Microvesicles in Alzheimer’s Disease Patients Identifies a miRNA Signature That May Impact Blood–Brain Barrier Integrity" @default.
- W4281481570 cites W1481406815 @default.
- W4281481570 cites W1580551725 @default.
- W4281481570 cites W1595078459 @default.
- W4281481570 cites W1751448311 @default.
- W4281481570 cites W1775513784 @default.
- W4281481570 cites W1815758482 @default.
- W4281481570 cites W1907833971 @default.
- W4281481570 cites W1982711630 @default.
- W4281481570 cites W1985402737 @default.
- W4281481570 cites W1987829395 @default.
- W4281481570 cites W1992033043 @default.
- W4281481570 cites W1997853371 @default.
- W4281481570 cites W2013638374 @default.
- W4281481570 cites W2023641365 @default.
- W4281481570 cites W2029514503 @default.
- W4281481570 cites W2029532402 @default.
- W4281481570 cites W2057082801 @default.
- W4281481570 cites W2066945106 @default.
- W4281481570 cites W2093878343 @default.
- W4281481570 cites W2098613848 @default.
- W4281481570 cites W2114104545 @default.
- W4281481570 cites W2114789645 @default.
- W4281481570 cites W2122866775 @default.
- W4281481570 cites W2124298589 @default.
- W4281481570 cites W2127274965 @default.
- W4281481570 cites W2134912780 @default.
- W4281481570 cites W2152239989 @default.
- W4281481570 cites W2154575244 @default.
- W4281481570 cites W2179438025 @default.
- W4281481570 cites W2198052523 @default.
- W4281481570 cites W2329803151 @default.
- W4281481570 cites W2468923595 @default.
- W4281481570 cites W2517693655 @default.
- W4281481570 cites W2525242879 @default.
- W4281481570 cites W2560409429 @default.
- W4281481570 cites W2588922139 @default.
- W4281481570 cites W2625216920 @default.
- W4281481570 cites W2642416886 @default.
- W4281481570 cites W2751416667 @default.
- W4281481570 cites W2756297365 @default.
- W4281481570 cites W2765662380 @default.
- W4281481570 cites W2775475677 @default.
- W4281481570 cites W2792497181 @default.
- W4281481570 cites W2796520211 @default.
- W4281481570 cites W2796568182 @default.
- W4281481570 cites W2799428347 @default.
- W4281481570 cites W2802141114 @default.
- W4281481570 cites W2804842714 @default.
- W4281481570 cites W2885053617 @default.
- W4281481570 cites W2897379107 @default.
- W4281481570 cites W2898726539 @default.
- W4281481570 cites W2899343204 @default.
- W4281481570 cites W2914106585 @default.
- W4281481570 cites W2916939313 @default.
- W4281481570 cites W2917207851 @default.
- W4281481570 cites W2921468251 @default.
- W4281481570 cites W2945288061 @default.
- W4281481570 cites W2952421891 @default.
- W4281481570 cites W2954807790 @default.
- W4281481570 cites W2963201491 @default.
- W4281481570 cites W2966017177 @default.
- W4281481570 cites W2966611313 @default.
- W4281481570 cites W2968889651 @default.
- W4281481570 cites W2970727076 @default.
- W4281481570 cites W2971792831 @default.
- W4281481570 cites W2975869948 @default.
- W4281481570 cites W2978421067 @default.
- W4281481570 cites W2979971613 @default.
- W4281481570 cites W2980736644 @default.
- W4281481570 cites W2980896086 @default.
- W4281481570 cites W2982982531 @default.
- W4281481570 cites W2986298043 @default.
- W4281481570 cites W2988774670 @default.
- W4281481570 cites W2992158676 @default.
- W4281481570 cites W2996444748 @default.
- W4281481570 cites W3000013851 @default.
- W4281481570 cites W3012324410 @default.
- W4281481570 cites W3034541330 @default.
- W4281481570 cites W3043716684 @default.
- W4281481570 cites W3045315553 @default.
- W4281481570 cites W3081169267 @default.
- W4281481570 cites W3086660927 @default.
- W4281481570 cites W3104051145 @default.
- W4281481570 cites W3111601802 @default.
- W4281481570 cites W3120483217 @default.