Matches in SemOpenAlex for { <https://semopenalex.org/work/W4281489712> ?p ?o ?g. }
- W4281489712 abstract "Abstract Calcium (Ca 2+ ) is well known as a second messenger in eukaryotes, where Ca 2+ signaling controls life-sustaining cellular processes. Although bacteria produce the components required for Ca 2+ signaling, little is known about the mechanisms of bacterial Ca 2+ signaling. Previously, we have identified a putative Ca 2+ -binding protein EfhP (PA4107) with two canonical EF-hand motifs and reported that EfhP mediates Ca 2+ regulation of virulence factors production and infectivity in Pseudomonas aeruginosa, a human pathogen causing life-threatening infections. Here, we show that EfhP selectively binds Ca 2+ with 13.7 µM affinity, and that mutations at the +X and −Z positions within each or both EF-hand motifs abolished Ca 2+ binding. We also show that the hydrophobicity of EfhP increased in a Ca 2+ - dependent manner, however no such response was detected in the mutated proteins. 15 N-NMR showed Ca 2+ -dependent chemical shifts in EfhP confirming Ca 2+ -binding triggered structural rearrangements in the protein. Deletion of efhP impaired P. aeruginosa survival in macrophages and virulence in vivo . Disabling EfhP Ca 2+ binding abolished Ca 2+ induction of pyocyanin production in vitro. These data confirm that EfhP selectively binds Ca 2+ , which triggers its structural changes required for the Ca 2+ regulation of P. aeruginosa virulence, thus establishing the role of EfhP as a Ca 2+ sensor." @default.
- W4281489712 created "2022-05-26" @default.
- W4281489712 creator A5002748315 @default.
- W4281489712 creator A5015347295 @default.
- W4281489712 creator A5021019658 @default.
- W4281489712 creator A5025316927 @default.
- W4281489712 creator A5030857978 @default.
- W4281489712 creator A5030952039 @default.
- W4281489712 creator A5035936638 @default.
- W4281489712 creator A5039782766 @default.
- W4281489712 creator A5039801730 @default.
- W4281489712 creator A5044153814 @default.
- W4281489712 creator A5049246903 @default.
- W4281489712 creator A5055936029 @default.
- W4281489712 creator A5065176157 @default.
- W4281489712 creator A5068805713 @default.
- W4281489712 creator A5069467530 @default.
- W4281489712 creator A5079209474 @default.
- W4281489712 creator A5082496642 @default.
- W4281489712 date "2022-05-25" @default.
- W4281489712 modified "2023-10-16" @default.
- W4281489712 title "EF-hand protein, EfhP, specifically binds Ca2+ and mediates Ca2+ regulation of virulence in a human pathogen Pseudomonas aeruginosa" @default.
- W4281489712 cites W1520922436 @default.
- W4281489712 cites W1585264254 @default.
- W4281489712 cites W1921710458 @default.
- W4281489712 cites W1965608446 @default.
- W4281489712 cites W1969971056 @default.
- W4281489712 cites W1971500643 @default.
- W4281489712 cites W1974019900 @default.
- W4281489712 cites W1977435315 @default.
- W4281489712 cites W1977587790 @default.
- W4281489712 cites W1977644290 @default.
- W4281489712 cites W1983312463 @default.
- W4281489712 cites W1988200986 @default.
- W4281489712 cites W1988870542 @default.
- W4281489712 cites W2001896124 @default.
- W4281489712 cites W2010955080 @default.
- W4281489712 cites W2018458023 @default.
- W4281489712 cites W2026807011 @default.
- W4281489712 cites W2034952964 @default.
- W4281489712 cites W2038900305 @default.
- W4281489712 cites W2039174337 @default.
- W4281489712 cites W2041445887 @default.
- W4281489712 cites W2044961004 @default.
- W4281489712 cites W2049667973 @default.
- W4281489712 cites W2051227373 @default.
- W4281489712 cites W2052298397 @default.
- W4281489712 cites W2052366943 @default.
- W4281489712 cites W2052460623 @default.
- W4281489712 cites W2055043387 @default.
- W4281489712 cites W2060685249 @default.
- W4281489712 cites W2062001409 @default.
- W4281489712 cites W2063405659 @default.
- W4281489712 cites W2064386449 @default.
- W4281489712 cites W2064644853 @default.
- W4281489712 cites W2065372703 @default.
- W4281489712 cites W2068297653 @default.
- W4281489712 cites W2071352334 @default.
- W4281489712 cites W2081186456 @default.
- W4281489712 cites W2085320887 @default.
- W4281489712 cites W2088767734 @default.
- W4281489712 cites W2091023135 @default.
- W4281489712 cites W2093155720 @default.
- W4281489712 cites W2094458603 @default.
- W4281489712 cites W2096525273 @default.
- W4281489712 cites W2096887440 @default.
- W4281489712 cites W2097606916 @default.
- W4281489712 cites W2097804984 @default.
- W4281489712 cites W2099952268 @default.
- W4281489712 cites W2111054934 @default.
- W4281489712 cites W2123358379 @default.
- W4281489712 cites W2124805544 @default.
- W4281489712 cites W2131093518 @default.
- W4281489712 cites W2133380824 @default.
- W4281489712 cites W2133985721 @default.
- W4281489712 cites W2137901394 @default.
- W4281489712 cites W2147575306 @default.
- W4281489712 cites W2149908911 @default.
- W4281489712 cites W2156748614 @default.
- W4281489712 cites W2164819852 @default.
- W4281489712 cites W2169821755 @default.
- W4281489712 cites W2179324586 @default.
- W4281489712 cites W2237937380 @default.
- W4281489712 cites W2311203695 @default.
- W4281489712 cites W2313422370 @default.
- W4281489712 cites W2334164495 @default.
- W4281489712 cites W2338854241 @default.
- W4281489712 cites W2434433181 @default.
- W4281489712 cites W2464144169 @default.
- W4281489712 cites W2532246160 @default.
- W4281489712 cites W2534475972 @default.
- W4281489712 cites W2540593770 @default.
- W4281489712 cites W2553030812 @default.
- W4281489712 cites W2560478592 @default.
- W4281489712 cites W2624937154 @default.
- W4281489712 cites W2799524357 @default.
- W4281489712 cites W2807161463 @default.
- W4281489712 cites W2888944359 @default.
- W4281489712 cites W2944193677 @default.
- W4281489712 cites W2982185911 @default.