Matches in SemOpenAlex for { <https://semopenalex.org/work/W4281561215> ?p ?o ?g. }
- W4281561215 endingPage "790" @default.
- W4281561215 startingPage "779" @default.
- W4281561215 abstract "Asian soybean rust, caused by the fungus Phakopsora pachyrhizi, is one of the most important diseases affecting soybean production in tropical areas. During infection, P. pachyrhizi secretes proteins from haustoria that are transferred into plant cells to promote virulence. To date, only one candidate P. pachyrhizi effector protein has been characterized in detail to understand the mechanism by which it suppresses plant defenses to enhance infection. Here, we aimed to extend understanding of the pathogenic mechanisms of P. pachyrhizi based on the discovery of host proteins that interact with the effector candidate Phapa-7431740. We demonstrated that Phapa-7431740 suppresses pathogen-associated molecular pattern-triggered immunity (PTI) and that it interacts with a soybean glucan endo-1,3-β-glucosidase (GmβGLU), a pathogenesis-related (PR) protein belonging to the PR-2 family. Structural and phylogenetic characterization of the PR-2 protein family predicted in the soybean genome and comparison to PR-2 family members in Arabidopsis thaliana and cotton, demonstrated that GmβGLU is a type IV β-1,3-glucanase. Transcriptional profiling during an infection time course showed that the GmβGLU mRNA is highly induced during the initial hours after infection, coinciding with peak of expression of Phapa-7431740. The effector was able to interfere with the activity of GmβGLU in vitro, with a dose-dependent inhibition. Our results suggest that Phapa-7431740 may suppress PTI by interfering with glucan endo-1,3-β-glucosidase activity. [Formula: see text] The author(s) have dedicated the work to the public domain under the Creative Commons CC0 No Rights Reserved license by waiving all of his or her rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law, 2022." @default.
- W4281561215 created "2022-05-27" @default.
- W4281561215 creator A5002763584 @default.
- W4281561215 creator A5013526995 @default.
- W4281561215 creator A5013866354 @default.
- W4281561215 creator A5014704956 @default.
- W4281561215 creator A5019674464 @default.
- W4281561215 creator A5022867784 @default.
- W4281561215 creator A5045856861 @default.
- W4281561215 creator A5047104610 @default.
- W4281561215 creator A5048150440 @default.
- W4281561215 creator A5048481678 @default.
- W4281561215 creator A5055713015 @default.
- W4281561215 creator A5056322652 @default.
- W4281561215 creator A5056980129 @default.
- W4281561215 creator A5081703730 @default.
- W4281561215 date "2022-09-01" @default.
- W4281561215 modified "2023-10-14" @default.
- W4281561215 title "A <i>Phakopsora pachyrhizi</i> Effector Suppresses PAMP-Triggered Immunity and Interacts with a Soybean Glucan Endo-1,3-β-Glucosidase to Promote Virulence" @default.
- W4281561215 cites W1553627850 @default.
- W4281561215 cites W1930194091 @default.
- W4281561215 cites W1970389917 @default.
- W4281561215 cites W1977075111 @default.
- W4281561215 cites W1979379629 @default.
- W4281561215 cites W1979613935 @default.
- W4281561215 cites W1986385142 @default.
- W4281561215 cites W1990184265 @default.
- W4281561215 cites W1995720527 @default.
- W4281561215 cites W1996677023 @default.
- W4281561215 cites W1997296794 @default.
- W4281561215 cites W2002368599 @default.
- W4281561215 cites W2004054644 @default.
- W4281561215 cites W2010113896 @default.
- W4281561215 cites W2019148401 @default.
- W4281561215 cites W2023145021 @default.
- W4281561215 cites W2024905753 @default.
- W4281561215 cites W2025186863 @default.
- W4281561215 cites W2029067233 @default.
- W4281561215 cites W2029227807 @default.
- W4281561215 cites W2031345106 @default.
- W4281561215 cites W2031991165 @default.
- W4281561215 cites W2035874210 @default.
- W4281561215 cites W2045074547 @default.
- W4281561215 cites W2046993625 @default.
- W4281561215 cites W2051965529 @default.
- W4281561215 cites W2054126408 @default.
- W4281561215 cites W2071898664 @default.
- W4281561215 cites W2074008446 @default.
- W4281561215 cites W2077376180 @default.
- W4281561215 cites W2078586425 @default.
- W4281561215 cites W2091817628 @default.
- W4281561215 cites W2093198909 @default.
- W4281561215 cites W2093381794 @default.
- W4281561215 cites W2099249565 @default.
- W4281561215 cites W2101713878 @default.
- W4281561215 cites W2106147903 @default.
- W4281561215 cites W2106258385 @default.
- W4281561215 cites W2108502123 @default.
- W4281561215 cites W2113964973 @default.
- W4281561215 cites W2119511951 @default.
- W4281561215 cites W2126797562 @default.
- W4281561215 cites W2132926880 @default.
- W4281561215 cites W2133123568 @default.
- W4281561215 cites W2138122982 @default.
- W4281561215 cites W2139617161 @default.
- W4281561215 cites W2141816565 @default.
- W4281561215 cites W2142298292 @default.
- W4281561215 cites W2149899130 @default.
- W4281561215 cites W2149912264 @default.
- W4281561215 cites W2153009023 @default.
- W4281561215 cites W2156558334 @default.
- W4281561215 cites W2171091522 @default.
- W4281561215 cites W2171468042 @default.
- W4281561215 cites W2187758452 @default.
- W4281561215 cites W2295141502 @default.
- W4281561215 cites W2297931152 @default.
- W4281561215 cites W2303783270 @default.
- W4281561215 cites W2472717230 @default.
- W4281561215 cites W2503171537 @default.
- W4281561215 cites W2526348439 @default.
- W4281561215 cites W2550186384 @default.
- W4281561215 cites W2550585340 @default.
- W4281561215 cites W2611668892 @default.
- W4281561215 cites W2730472814 @default.
- W4281561215 cites W2759370753 @default.
- W4281561215 cites W2761049005 @default.
- W4281561215 cites W2766400560 @default.
- W4281561215 cites W2795646901 @default.
- W4281561215 cites W2890545725 @default.
- W4281561215 cites W2890643608 @default.
- W4281561215 cites W2892836180 @default.
- W4281561215 cites W2898402099 @default.
- W4281561215 cites W2945277748 @default.
- W4281561215 cites W2997583280 @default.
- W4281561215 cites W2997867652 @default.
- W4281561215 cites W3001951030 @default.
- W4281561215 cites W3005235420 @default.
- W4281561215 cites W3091993752 @default.