Matches in SemOpenAlex for { <https://semopenalex.org/work/W4281568286> ?p ?o ?g. }
- W4281568286 endingPage "2250" @default.
- W4281568286 startingPage "2233" @default.
- W4281568286 abstract "Exposure to solar UV irradiation damages γ-crystallin, leading to cataract formation via aggregation. α-Crystallin, as a small heat shock protein, efficiently suppresses this irreversible aggregation by selectively binding the denatured γ-crystallin monomer. In this study, liquid chromatography tandem mass spectrometry was used to evaluate UV-325 nm irradiation-induced photodamage of human γD-crystallin in the presence of bovine α-crystallin, atomic force microscope (AFM) and dynamic light scattering (DLS) techniques were used to detect the quaternary structure changes of the α-crystallin oligomer, and Fourier transform infrared spectroscopy and temperature-jump nanosecond time-resolved IR absorbance difference spectroscopy were used to probe the secondary structure changes of bovine α-crystallin. We find that the thermal-induced subunit dissociation of the α-crystallin oligomer involves the breaking of hydrogen bonds at the dimeric interface, leading to three different spectral components at varied temperature regions as resolved from temperature-dependent IR spectra. Under UV-325 nm irradiation, unfolded γD-crystallin binds to the dissociated α-crystallin subunit to form an αγ-complex, then follows the reassociation of the αγ-complex to the partially dissociated α-crystallin oligomer. This prevents the aggregation of denatured γD-crystallin. The formation of the γD-bound α-crystallin oligomer is further confirmed by AFM and DLS analysis, which reveals an obvious size expansion in the reassociated αγ-oligomers. In addition, UV-325 nm irradiation causes a peptide bond cleavage of γD-crystallin at Ala158 in the presence of α-crystallin. Our results suggest a very effective protection mechanism for subunits dissociated from α-crystallin oligomers against UV irradiation-induced aggregation of γD-crystallin, at the expense of a loss of a short C-terminal peptide in γD-crystallin." @default.
- W4281568286 created "2022-05-27" @default.
- W4281568286 creator A5002111219 @default.
- W4281568286 creator A5007615595 @default.
- W4281568286 creator A5012932406 @default.
- W4281568286 creator A5019560977 @default.
- W4281568286 creator A5024753658 @default.
- W4281568286 creator A5051728653 @default.
- W4281568286 creator A5077366714 @default.
- W4281568286 creator A5080371430 @default.
- W4281568286 date "2022-06-01" @default.
- W4281568286 modified "2023-10-15" @default.
- W4281568286 title "The mechanism for thermal-enhanced chaperone-like activity of α-crystallin against UV irradiation-induced aggregation of γD-crystallin" @default.
- W4281568286 cites W1501703174 @default.
- W4281568286 cites W1518684523 @default.
- W4281568286 cites W1523027652 @default.
- W4281568286 cites W1555856681 @default.
- W4281568286 cites W1599376419 @default.
- W4281568286 cites W1973730075 @default.
- W4281568286 cites W1974229917 @default.
- W4281568286 cites W1974430858 @default.
- W4281568286 cites W1974782304 @default.
- W4281568286 cites W1980031953 @default.
- W4281568286 cites W1983790438 @default.
- W4281568286 cites W1986301747 @default.
- W4281568286 cites W1988230012 @default.
- W4281568286 cites W1992045220 @default.
- W4281568286 cites W1996418015 @default.
- W4281568286 cites W1998495384 @default.
- W4281568286 cites W2001584915 @default.
- W4281568286 cites W2001952240 @default.
- W4281568286 cites W2002121950 @default.
- W4281568286 cites W2002602401 @default.
- W4281568286 cites W2003636651 @default.
- W4281568286 cites W2004688505 @default.
- W4281568286 cites W2007780468 @default.
- W4281568286 cites W2010104818 @default.
- W4281568286 cites W2012490977 @default.
- W4281568286 cites W2015524724 @default.
- W4281568286 cites W2016011421 @default.
- W4281568286 cites W2019453308 @default.
- W4281568286 cites W2021037818 @default.
- W4281568286 cites W2039499792 @default.
- W4281568286 cites W2040211368 @default.
- W4281568286 cites W2040743789 @default.
- W4281568286 cites W2044031095 @default.
- W4281568286 cites W2044579912 @default.
- W4281568286 cites W2046786952 @default.
- W4281568286 cites W2055125957 @default.
- W4281568286 cites W2055597670 @default.
- W4281568286 cites W2056959848 @default.
- W4281568286 cites W2057294351 @default.
- W4281568286 cites W2062096563 @default.
- W4281568286 cites W2065376598 @default.
- W4281568286 cites W2065388396 @default.
- W4281568286 cites W2070019985 @default.
- W4281568286 cites W2079702727 @default.
- W4281568286 cites W2083038393 @default.
- W4281568286 cites W2086963517 @default.
- W4281568286 cites W2091584330 @default.
- W4281568286 cites W2092457909 @default.
- W4281568286 cites W2093787245 @default.
- W4281568286 cites W2094568136 @default.
- W4281568286 cites W2108107378 @default.
- W4281568286 cites W2119675439 @default.
- W4281568286 cites W2128064586 @default.
- W4281568286 cites W2135987830 @default.
- W4281568286 cites W213636671 @default.
- W4281568286 cites W2138456893 @default.
- W4281568286 cites W2139512993 @default.
- W4281568286 cites W2140917307 @default.
- W4281568286 cites W2140918764 @default.
- W4281568286 cites W2143227193 @default.
- W4281568286 cites W2157542014 @default.
- W4281568286 cites W2159480111 @default.
- W4281568286 cites W2160380154 @default.
- W4281568286 cites W2160564267 @default.
- W4281568286 cites W2164060511 @default.
- W4281568286 cites W2166478943 @default.
- W4281568286 cites W22234875 @default.
- W4281568286 cites W2321113341 @default.
- W4281568286 cites W2593267197 @default.
- W4281568286 cites W2593384038 @default.
- W4281568286 cites W2803205598 @default.
- W4281568286 cites W2807249662 @default.
- W4281568286 cites W2899077341 @default.
- W4281568286 cites W2969602950 @default.
- W4281568286 cites W2969745878 @default.
- W4281568286 cites W2990488105 @default.
- W4281568286 cites W2993166999 @default.
- W4281568286 cites W3023827785 @default.
- W4281568286 cites W3036122925 @default.
- W4281568286 cites W4251647708 @default.
- W4281568286 cites W802482256 @default.
- W4281568286 cites W984320018 @default.
- W4281568286 cites W2919884148 @default.
- W4281568286 doi "https://doi.org/10.1016/j.bpj.2022.05.032" @default.
- W4281568286 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35619565" @default.