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• W4281569160 endingPage "738393" @default.
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• W4281569160 abstract "High-throughput genotyping tools are essential for genetic research and genomic selection applications, especially in economically important species. In this study, genotyping by target sequencing (GBTS) system and capture-in-solution technology were utilized to develop a multiple single-nucleotide polymorphism (mSNP) liquid array, named “Baoxin-I,” for genome-wide evaluation of allelic variation in Pacific abalone (Haliotis discus hannai), an economically important mollusk species widely farmed in East Asia. A total of 1059 Pacific abalone individuals were selected for whole-genome resequencing and over 1.67 million SNPs were identified. A subset of 87,959 SNPs that were evenly distributed across the genome were selected from the 40-K target genomic regions. The performance evaluation of Baoxin-I was conducted with a commercial Pacific abalone population, two closely related Haliotis species, and one hybrid. In total, 73.66% of the array loci were of high quality and could be successfully genotyped with 97.41% calling rates, with a 54.26%–94.94% polymorphic SNP rate across species. The Baoxin-I SNP array was then applied in a population separation test and a genomic selection program to validate efficiency and accuracy: (1) the population separation test showed that Baoxin-I could distinguish four closely species; and (2) the prediction accuracy for 10 growth-related traits of Pacific abalone obtained using Baoxin-I genotyping data was consistent with that obtained using whole-genomic resequencing data, validating the performance of Baoxin-I in genomic selection. In summary, the Baoxin-I SNP array will provide a useful platform for high-quality genotyping that may be beneficial to the genomic selective breeding of Pacific abalone." @default.
• W4281569160 created "2022-05-27" @default.
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• W4281569160 date "2022-09-01" @default.
• W4281569160 modified "2023-09-28" @default.
• W4281569160 title "Development and validation of a 40-K multiple-SNP array for Pacific abalone (Haliotis discus hannai)" @default.
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• W4281569160 doi "https://doi.org/10.1016/j.aquaculture.2022.738393" @default.
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