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- W4281569663 abstract "The infections of chicken coccidiosis impact the welfare of chickens and the economical production of poultry. Eimeria mitis is ubiquitous in chicken coccidiosis, and E. mitis infection can significantly affect the productivity of birds. Up to now, few efficient vaccines against E. mitis have been reported, whereas the recombinant subunit vaccines delivered by nanomaterials may elicit an encouraging outcome. Thus, in this study, we chose E. mitis 1a (Em1a) protein as the candidate antigen to generate Em1a preparations. The recombinant Em1a (rEm1a) protein was encapsulated with poly lactic-co-glycolic acid (PLGA) and chitosan (CS) nanospheres. The physical characterization of the rEm1a-PLGA and rEm1a-CS nanospheres was investigated, and the resulting nanospheres were proven to be nontoxic. The protective efficacy of rEm1a-PLGA and rEm1a-CS preparations was evaluated in E. mitis-challenged birds in comparison with two preparations containing rEm1a antigen emulsified in commercially available adjuvants. ELISA assay, flow cytometry analysis, and quantitative real-time PCR (qPCR) analysis indicated that vaccination with rEm1a-loaded nanospheres significantly upregulated the secretions of antibodies and cytokines and proportions of CD4+ and CD8+ T lymphocytes. Compared with the other three preparations, rEm1a-PLGA nanosphere was more effective in improving growth performance and inhibiting oocyst output in feces, indicating that the PLGA nanosphere was associated with optimal protection against E. mitis. Collectively, our results highlighted the advantages of nanovaccine in eliciting protective immunity and may provide a new perspective for developing effective vaccines against chicken coccidiosis." @default.
- W4281569663 created "2022-05-27" @default.
- W4281569663 creator A5033133176 @default.
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- W4281569663 date "2022-05-26" @default.
- W4281569663 modified "2023-10-04" @default.
- W4281569663 title "PLGA Nanospheres as Delivery Platforms for Eimeria mitis 1a Protein: A Novel Strategy to Improve Specific Immunity" @default.
- W4281569663 cites W1781424521 @default.
- W4281569663 cites W1964617663 @default.
- W4281569663 cites W1971645600 @default.
- W4281569663 cites W1976995229 @default.
- W4281569663 cites W1977039816 @default.
- W4281569663 cites W1985443295 @default.
- W4281569663 cites W1987263704 @default.
- W4281569663 cites W1987551271 @default.
- W4281569663 cites W1987576929 @default.
- W4281569663 cites W1989309678 @default.
- W4281569663 cites W1993392420 @default.
- W4281569663 cites W1996490750 @default.
- W4281569663 cites W2001863331 @default.
- W4281569663 cites W2004030143 @default.
- W4281569663 cites W2007487171 @default.
- W4281569663 cites W2012851891 @default.
- W4281569663 cites W2017852296 @default.
- W4281569663 cites W2018296894 @default.
- W4281569663 cites W2024490058 @default.
- W4281569663 cites W2038621898 @default.
- W4281569663 cites W2040165635 @default.
- W4281569663 cites W2041164141 @default.
- W4281569663 cites W2041489554 @default.
- W4281569663 cites W2044663689 @default.
- W4281569663 cites W2047186860 @default.
- W4281569663 cites W2048039920 @default.
- W4281569663 cites W2050459209 @default.
- W4281569663 cites W2051402309 @default.
- W4281569663 cites W2052873213 @default.
- W4281569663 cites W2056330722 @default.
- W4281569663 cites W2056340208 @default.
- W4281569663 cites W2057320783 @default.
- W4281569663 cites W2068586926 @default.
- W4281569663 cites W2073396962 @default.
- W4281569663 cites W2077292485 @default.
- W4281569663 cites W2077883112 @default.
- W4281569663 cites W2098337093 @default.
- W4281569663 cites W2113967448 @default.
- W4281569663 cites W2114245966 @default.
- W4281569663 cites W2116004188 @default.
- W4281569663 cites W2117630026 @default.
- W4281569663 cites W2136791826 @default.
- W4281569663 cites W2148658155 @default.
- W4281569663 cites W2152014155 @default.
- W4281569663 cites W2156112012 @default.
- W4281569663 cites W2158224546 @default.
- W4281569663 cites W2165116301 @default.
- W4281569663 cites W2169409695 @default.
- W4281569663 cites W2203910881 @default.
- W4281569663 cites W2216203568 @default.
- W4281569663 cites W2229086138 @default.
- W4281569663 cites W2264544376 @default.
- W4281569663 cites W2314625788 @default.
- W4281569663 cites W2320855920 @default.
- W4281569663 cites W2326733970 @default.
- W4281569663 cites W2341851664 @default.
- W4281569663 cites W2414943544 @default.
- W4281569663 cites W2505159449 @default.
- W4281569663 cites W2517664818 @default.
- W4281569663 cites W2558901993 @default.
- W4281569663 cites W2593998514 @default.
- W4281569663 cites W2773527998 @default.
- W4281569663 cites W2784110492 @default.
- W4281569663 cites W2791655169 @default.
- W4281569663 cites W2793060627 @default.
- W4281569663 cites W2803300773 @default.
- W4281569663 cites W2804601230 @default.
- W4281569663 cites W2809636208 @default.
- W4281569663 cites W2809757546 @default.
- W4281569663 cites W2889535285 @default.
- W4281569663 cites W2919350624 @default.
- W4281569663 cites W2944018161 @default.
- W4281569663 cites W2981632193 @default.
- W4281569663 cites W2984379948 @default.
- W4281569663 cites W2990139186 @default.
- W4281569663 cites W2999790546 @default.
- W4281569663 cites W3000743283 @default.
- W4281569663 cites W3008332102 @default.
- W4281569663 cites W3008365548 @default.
- W4281569663 cites W3012247520 @default.
- W4281569663 cites W3034086700 @default.
- W4281569663 cites W3082854752 @default.
- W4281569663 cites W3084637486 @default.
- W4281569663 cites W3133498558 @default.
- W4281569663 cites W3134517376 @default.
- W4281569663 cites W3137801873 @default.