FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4281623408> ?p ?o ?g. }

• W4281623408 abstract "Daily physiologic loading causes fluctuations in hydration of the intervertebral disc (IVD); thus, the embedded cells experience cyclic alterations to their osmotic environment. These osmotic fluctuations have been described as a mechanism linking mechanics and biology, and have previously been shown to promote biosynthesis in chondrocytes. However, this phenomenon has yet to be fully interrogated in the IVD. Additionally, the specialized extracellular matrix surrounding the cells, the pericellular matrix (PCM), transduces the biophysical signals that cells ultimately experience. While it is known that the PCM is altered in disc degeneration, whether it disrupts normal osmotic mechanotransduction has yet to be determined. Thus, our objectives were to assess: (1) whether dynamic osmotic conditions stimulate biosynthesis in nucleus pulposus cells, and (2) whether pericellular heparan sulfate proteoglycans (HSPGs) modulate the biosynthetic response to osmotic loading.Bovine nucleus pulposus cells isolated with retained PCM were encapsulated in 1.5% alginate beads and treated with or without heparinase III, an enzyme that degrades the pericellular HSPGs. Beads were subjected to 1 h of daily iso-osmotic, hyper-osmotic, or hypo-osmotic loading for 1, 2, or 4 weeks. At each timepoint the total amount of extracellular and pericellular sGAG/DNA were quantified. Additionally, whether osmotic loading triggered alterations to HSPG sulfation was assessed via immunohistochemistry for the heparan sulfate 6-O-sulfertransferase 1 (HS6ST1) enzyme.Osmotic loading significantly influenced sGAG/DNA accumulation with a hyper-osmotic change promoting the greatest sGAG/DNA accumulation in the pericellular region compared with iso-osmotic conditions. Heparanase-III treatment significantly reduced extracellular sGAG/DNA but pericellular sGAG was not affected. HS6ST1 expression was not affected by osmotic loading.Results suggest that hyper-osmotic loading promotes matrix synthesis and that modifications to HSPGs directly influence the metabolic responses of cells to osmotic fluctuations. Collectively, results suggest degeneration-associated modifications to pericellular HSPGs may contribute to the altered mechanobiology observed in disease." @default.
• W4281623408 created "2022-06-13" @default.
• W4281623408 creator A5016387689 @default.
• W4281623408 creator A5016829233 @default.
• W4281623408 creator A5032172599 @default.
• W4281623408 creator A5041975324 @default.
• W4281623408 creator A5070640679 @default.
• W4281623408 date "2022-06-01" @default.
• W4281623408 modified "2023-10-16" @default.
• W4281623408 title "Pericellular heparan sulfate proteoglycans: Role in regulating the biosynthetic response of nucleus pulposus cells to osmotic loading" @default.
• W4281623408 cites W1175185197 @default.
• W4281623408 cites W127220886 @default.
• W4281623408 cites W1902204700 @default.
• W4281623408 cites W1966686938 @default.
• W4281623408 cites W1974537097 @default.
• W4281623408 cites W1983540758 @default.
• W4281623408 cites W2007029906 @default.
• W4281623408 cites W2010240830 @default.
• W4281623408 cites W2011931614 @default.
• W4281623408 cites W2016892282 @default.
• W4281623408 cites W2031116764 @default.
• W4281623408 cites W2033620253 @default.
• W4281623408 cites W2037806019 @default.
• W4281623408 cites W2046543724 @default.
• W4281623408 cites W2052291791 @default.
• W4281623408 cites W2053117566 @default.
• W4281623408 cites W2053877508 @default.
• W4281623408 cites W2055884129 @default.
• W4281623408 cites W2060278780 @default.
• W4281623408 cites W2060559986 @default.
• W4281623408 cites W2071206980 @default.
• W4281623408 cites W2084835488 @default.
• W4281623408 cites W2086898489 @default.
• W4281623408 cites W2091025894 @default.
• W4281623408 cites W2096573916 @default.
• W4281623408 cites W2105367621 @default.
• W4281623408 cites W2142259793 @default.
• W4281623408 cites W2155936356 @default.
• W4281623408 cites W2159255182 @default.
• W4281623408 cites W2174709531 @default.
• W4281623408 cites W2266884832 @default.
• W4281623408 cites W2325277685 @default.
• W4281623408 cites W236611178 @default.
• W4281623408 cites W2466000789 @default.
• W4281623408 cites W2474088718 @default.
• W4281623408 cites W2504171339 @default.
• W4281623408 cites W2514574167 @default.
• W4281623408 cites W2566139987 @default.
• W4281623408 cites W2593955381 @default.
• W4281623408 cites W2734755718 @default.
• W4281623408 cites W2788945808 @default.
• W4281623408 cites W2804160472 @default.
• W4281623408 cites W2809997034 @default.
• W4281623408 cites W2904576813 @default.
• W4281623408 cites W3017075860 @default.
• W4281623408 cites W3105186113 @default.
• W4281623408 cites W3108963123 @default.
• W4281623408 cites W3112008314 @default.
• W4281623408 cites W3123153087 @default.
• W4281623408 doi "https://doi.org/10.1002/jsp2.1209" @default.
• W4281623408 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35783912" @default.
• W4281623408 hasPublicationYear "2022" @default.
• W4281623408 type Work @default.
• W4281623408 citedByCount "0" @default.
• W4281623408 crossrefType "journal-article" @default.
• W4281623408 hasAuthorship W4281623408A5016387689 @default.
• W4281623408 hasAuthorship W4281623408A5016829233 @default.
• W4281623408 hasAuthorship W4281623408A5032172599 @default.
• W4281623408 hasAuthorship W4281623408A5041975324 @default.
• W4281623408 hasAuthorship W4281623408A5070640679 @default.
• W4281623408 hasBestOaLocation W42816234083 @default.
• W4281623408 hasConcept C104317684 @default.
• W4281623408 hasConcept C12554922 @default.
• W4281623408 hasConcept C129900496 @default.
• W4281623408 hasConcept C153074725 @default.
• W4281623408 hasConcept C168529131 @default.
• W4281623408 hasConcept C181104049 @default.
• W4281623408 hasConcept C185592680 @default.
• W4281623408 hasConcept C189165786 @default.
• W4281623408 hasConcept C2777478136 @default.
• W4281623408 hasConcept C2778041096 @default.
• W4281623408 hasConcept C2779335624 @default.
• W4281623408 hasConcept C2780723820 @default.
• W4281623408 hasConcept C28406088 @default.
• W4281623408 hasConcept C55493867 @default.
• W4281623408 hasConcept C86803240 @default.
• W4281623408 hasConcept C95444343 @default.
• W4281623408 hasConcept C9603476 @default.
• W4281623408 hasConceptScore W4281623408C104317684 @default.
• W4281623408 hasConceptScore W4281623408C12554922 @default.
• W4281623408 hasConceptScore W4281623408C129900496 @default.
• W4281623408 hasConceptScore W4281623408C153074725 @default.
• W4281623408 hasConceptScore W4281623408C168529131 @default.
• W4281623408 hasConceptScore W4281623408C181104049 @default.
• W4281623408 hasConceptScore W4281623408C185592680 @default.
• W4281623408 hasConceptScore W4281623408C189165786 @default.
• W4281623408 hasConceptScore W4281623408C2777478136 @default.
• W4281623408 hasConceptScore W4281623408C2778041096 @default.
• W4281623408 hasConceptScore W4281623408C2779335624 @default.
• W4281623408 hasConceptScore W4281623408C2780723820 @default.

• next