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• W4281623814 endingPage "1832" @default.
• W4281623814 startingPage "1832" @default.
• W4281623814 abstract "Proliferating cell nuclear antigen (PCNA) is a DNA clamp that functions in key roles for DNA replication and repair. After the completion of DNA synthesis, PCNA should be unloaded from DNA in a timely way. The ATAD5-RFC-Like Complex (ATAD5-RLC) unloads PCNA from DNA. However, the mechanism of the PCNA-unloading process remains unclear. In this study, we determined the minimal PCNA-unloading domain (ULD) of ATAD5. We identified several motifs in the ATAD5 ULD that are essential in the PCNA-unloading process. The C-terminus of ULD is required for the stable association of RFC2-5 for active RLC formation. The N-terminus of ULD participates in the opening of the PCNA ring. ATAD5-RLC was more robustly bound to open-liable PCNA compared to the wild type. These results suggest that distinct motifs of the ATAD5 ULD participate in each step of the PCNA-unloading process." @default.
• W4281623814 created "2022-06-13" @default.
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• W4281623814 date "2022-06-03" @default.
• W4281623814 modified "2023-09-26" @default.
• W4281623814 title "Distinct Motifs in ATAD5 C-Terminal Domain Modulate PCNA Unloading Process" @default.
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• W4281623814 doi "https://doi.org/10.3390/cells11111832" @default.
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