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• W4281625900 endingPage "3312" @default.
• W4281625900 startingPage "3312" @default.
• W4281625900 abstract "In the tissue donation field, to prevent pathogen transmission, all donors are screened by postmortem swabs for SARS-CoV-2 using qRT-PCR. Corneas from donors who tested positive for SARS-CoV-2 were subjected to further investigations. Corneal transplants and culture medium from positive donors were cultured under appropriate safety conditions for further analyses. Cornea tissue samples, including sclera/limbus/cornea, and culture media were taken at different time points for testing for SARS-CoV-2 using qRT-PCR, immunohistochemistry (IHC) and subgenomic RNA (sgRNA) analysis. Between January and May 2021, in four donors with initial negative premortem rapid tests, SARS-CoV-2 was detected post-mortem using qRT-PCR. In these cases, SARS-CoV-2 was observed at the beginning of cultivation in both tissue and culture medium using qRT-PCR and IHC. The virus was mainly localized in the limbus epithelial cells, with a stable detection level. Premortem rapid tests are potentially insufficient to exclude SARS-CoV-2 infection in corneal donors. While, for SARS-CoV-2, the risk of infection via transplants is considered low, a residual risk remains for presymptomatic new infections. However, our investigations provide the first indications that, with organ cultures, the risk of virus transmission is minimized due to the longer minimum culture period." @default.
• W4281625900 created "2022-06-13" @default.
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• W4281625900 date "2022-06-09" @default.
• W4281625900 modified "2023-10-14" @default.
• W4281625900 title "New Safety Aspects in Corneal Donation—Studies on SARS-CoV-2-Positive Corneal Donors" @default.
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• W4281625900 doi "https://doi.org/10.3390/jcm11123312" @default.
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