Matches in SemOpenAlex for { <https://semopenalex.org/work/W4281677362> ?p ?o ?g. }
- W4281677362 abstract "TMEM16F, a Ca2+-activated phospholipid scramblase (CaPLSase), is critical for placental trophoblast syncytialization, HIV infection, and SARS-CoV2-mediated syncytialization, however, how TMEM16F is activated during cell fusion is unclear. Here, using trophoblasts as a model for cell fusion, we demonstrate that Ca2+ influx through the Ca2+ permeable transient receptor potential vanilloid channel TRPV4 is critical for TMEM16F activation and plays a role in subsequent human trophoblast fusion. GSK1016790A, a TRPV4 specific agonist, robustly activates TMEM16F in trophoblasts. We also show that TRPV4 and TMEM16F are functionally coupled within Ca2+ microdomains in a human trophoblast cell line using patch-clamp electrophysiology. Pharmacological inhibition or gene silencing of TRPV4 hinders TMEM16F activation and subsequent trophoblast syncytialization. Our study uncovers the functional expression of TRPV4 and one of the physiological activation mechanisms of TMEM16F in human trophoblasts, thus providing us with novel strategies to regulate CaPLSase activity as a critical checkpoint of physiologically and disease-relevant cell fusion events." @default.
- W4281677362 created "2022-06-13" @default.
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- W4281677362 date "2022-06-07" @default.
- W4281677362 modified "2023-10-01" @default.
- W4281677362 title "Functional coupling between TRPV4 channel and TMEM16F modulates human trophoblast fusion" @default.
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- W4281677362 cites W1982929403 @default.
- W4281677362 cites W1982966384 @default.
- W4281677362 cites W1986130046 @default.
- W4281677362 cites W2000178601 @default.
- W4281677362 cites W2007307680 @default.
- W4281677362 cites W2011557706 @default.
- W4281677362 cites W2032921167 @default.
- W4281677362 cites W2046770351 @default.
- W4281677362 cites W2051023125 @default.
- W4281677362 cites W2067614576 @default.
- W4281677362 cites W2067845186 @default.
- W4281677362 cites W2076951847 @default.
- W4281677362 cites W2086589360 @default.
- W4281677362 cites W2091694654 @default.
- W4281677362 cites W2099485287 @default.
- W4281677362 cites W2099916828 @default.
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- W4281677362 cites W2291071386 @default.
- W4281677362 cites W2324398577 @default.
- W4281677362 cites W2345969966 @default.
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- W4281677362 cites W2558609663 @default.
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- W4281677362 cites W2996045117 @default.
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- W4281677362 cites W3018930742 @default.
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- W4281677362 doi "https://doi.org/10.7554/elife.78840" @default.
- W4281677362 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35670667" @default.
- W4281677362 hasPublicationYear "2022" @default.
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