FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4281692783> ?p ?o ?g. }

• W4281692783 abstract "The small ubiquitin-like modifier (SUMO) is involved in various cellular processes and mediates known non-covalent protein-protein interactions by three distinct binding surfaces, whose interactions are termed class I to class III. While interactors for the class I interaction, which involves binding of a SUMO-interacting motif (SIM) to a hydrophobic groove in SUMO-1 and SUMO-2/3, are widely abundant, only a couple of examples have been reported for the other two types of interactions. Class II binding is conveyed by the E67 loop region on SUMO-1. Many previous studies to identify SUMO binders using pull-down or microarray approaches did not strategize on the SUMO binding mode. Identification of SUMO binding partners is further complicated due to the typically transient and low affinity interactions with the modifier. Here we aimed to identify SUMO-1 binders selectively enriched for class II binding. Using a genetically encoded photo-crosslinker approach, we have designed SUMO-1 probes to covalently capture class II SUMO-1 interactors by strategically positioning the photo-crosslinking moiety on the SUMO-1 surface. The probes were validated using known class II and class I binding partners. We utilized the probe with p-benzoyl-phenylalanine (BzF, also termed BpF or Bpa) at the position of Gln69 to identify binding proteins from mammalian cell extracts using mass spectrometry. By comparison with results obtained with a similarly designed SUMO-1 probe to target SIM-mediated binders of the class I type, we identified 192 and 96 proteins specifically enriched by either probe, respectively. The implicated preferential class I or class II binding modes of these proteins will further contribute to unveiling the complex interplay of SUMO-1-mediated interactions." @default.
• W4281692783 created "2022-06-13" @default.
• W4281692783 creator A5005528827 @default.
• W4281692783 creator A5035521480 @default.
• W4281692783 creator A5039434936 @default.
• W4281692783 creator A5068939889 @default.
• W4281692783 creator A5079246402 @default.
• W4281692783 date "2022-05-30" @default.
• W4281692783 modified "2023-09-30" @default.
• W4281692783 title "A Photo-Crosslinking Approach to Identify Class II SUMO-1 Binders" @default.
• W4281692783 cites W1923637409 @default.
• W4281692783 cites W1959908262 @default.
• W4281692783 cites W1963910961 @default.
• W4281692783 cites W1967707683 @default.
• W4281692783 cites W1978339876 @default.
• W4281692783 cites W1990918213 @default.
• W4281692783 cites W2004722555 @default.
• W4281692783 cites W2013108773 @default.
• W4281692783 cites W2015848423 @default.
• W4281692783 cites W2035143588 @default.
• W4281692783 cites W2039741753 @default.
• W4281692783 cites W2043369995 @default.
• W4281692783 cites W2045941135 @default.
• W4281692783 cites W2053455848 @default.
• W4281692783 cites W2064723340 @default.
• W4281692783 cites W2070040136 @default.
• W4281692783 cites W2070182263 @default.
• W4281692783 cites W2077785805 @default.
• W4281692783 cites W2080752012 @default.
• W4281692783 cites W2085458306 @default.
• W4281692783 cites W2087087669 @default.
• W4281692783 cites W2091602394 @default.
• W4281692783 cites W2135965804 @default.
• W4281692783 cites W2148313902 @default.
• W4281692783 cites W2150986320 @default.
• W4281692783 cites W2159675211 @default.
• W4281692783 cites W2167060626 @default.
• W4281692783 cites W2167536088 @default.
• W4281692783 cites W2168830189 @default.
• W4281692783 cites W2170776626 @default.
• W4281692783 cites W2342922422 @default.
• W4281692783 cites W2343816017 @default.
• W4281692783 cites W2403400866 @default.
• W4281692783 cites W2463195069 @default.
• W4281692783 cites W2490844245 @default.
• W4281692783 cites W2567575834 @default.
• W4281692783 cites W2582931844 @default.
• W4281692783 cites W2593321113 @default.
• W4281692783 cites W2597003300 @default.
• W4281692783 cites W2606317386 @default.
• W4281692783 cites W2950764390 @default.
• W4281692783 cites W2968185949 @default.
• W4281692783 cites W2974530247 @default.
• W4281692783 cites W2993213413 @default.
• W4281692783 cites W3043639762 @default.
• W4281692783 cites W3046257048 @default.
• W4281692783 cites W3107527779 @default.
• W4281692783 cites W3110917833 @default.
• W4281692783 cites W3121800253 @default.
• W4281692783 cites W3208691473 @default.
• W4281692783 cites W4210245887 @default.
• W4281692783 doi "https://doi.org/10.3389/fchem.2022.900989" @default.
• W4281692783 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35707458" @default.
• W4281692783 hasPublicationYear "2022" @default.
• W4281692783 type Work @default.
• W4281692783 citedByCount "1" @default.
• W4281692783 countsByYear W42816927832023 @default.
• W4281692783 crossrefType "journal-article" @default.
• W4281692783 hasAuthorship W4281692783A5005528827 @default.
• W4281692783 hasAuthorship W4281692783A5035521480 @default.
• W4281692783 hasAuthorship W4281692783A5039434936 @default.
• W4281692783 hasAuthorship W4281692783A5068939889 @default.
• W4281692783 hasAuthorship W4281692783A5079246402 @default.
• W4281692783 hasBestOaLocation W42816927831 @default.
• W4281692783 hasConcept C104317684 @default.
• W4281692783 hasConcept C107824862 @default.
• W4281692783 hasConcept C11804247 @default.
• W4281692783 hasConcept C12554922 @default.
• W4281692783 hasConcept C171350616 @default.
• W4281692783 hasConcept C178790620 @default.
• W4281692783 hasConcept C180577832 @default.
• W4281692783 hasConcept C185592680 @default.
• W4281692783 hasConcept C25602115 @default.
• W4281692783 hasConcept C2776568683 @default.
• W4281692783 hasConcept C51639874 @default.
• W4281692783 hasConcept C55493867 @default.
• W4281692783 hasConcept C70721500 @default.
• W4281692783 hasConcept C71240020 @default.
• W4281692783 hasConcept C86339819 @default.
• W4281692783 hasConcept C86803240 @default.
• W4281692783 hasConcept C94966510 @default.
• W4281692783 hasConceptScore W4281692783C104317684 @default.
• W4281692783 hasConceptScore W4281692783C107824862 @default.
• W4281692783 hasConceptScore W4281692783C11804247 @default.
• W4281692783 hasConceptScore W4281692783C12554922 @default.
• W4281692783 hasConceptScore W4281692783C171350616 @default.
• W4281692783 hasConceptScore W4281692783C178790620 @default.
• W4281692783 hasConceptScore W4281692783C180577832 @default.
• W4281692783 hasConceptScore W4281692783C185592680 @default.
• W4281692783 hasConceptScore W4281692783C25602115 @default.

• next