FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4281695964> ?p ?o ?g. }

• W4281695964 abstract "Studies on biological functions of N6-methyladenosine (m6 A) modification in mRNA have sprung up in recent years. Previous studies have reported m6 A can determine mRNA fate and play a pivotal role in tumour development and progression. The zinc finger protein 677 (ZNF677) belongs to the zinc finger protein family and possesses transcription factor activity by binding sequence-specific DNA.The expression of ZNF677 and its clinicopathological impact were evaluated in renal cell carcinoma (RCC) patients. The m6 A level of ZNF677 was determined by m6 A methylated RNA immunoprecipitation-sequencing (MeRIP-seq) and MeRIP-qPCR in RCC tissues and adjacent normal tissues. RNA immunoprecipitation-qPCR (RIP-qPCR) and luciferase assays were performed to identify the targeted effect of IGF2BP2 and YTHDF1 on ZNF677. RCC cells and subcutaneous models uncovered the role of ZNF677 methylated by CRISPR/dCas13b-METTL3 in tumour growth. ZNF677-binding sites in the CDKN3 promoter were investigated by chromatin immunoprecipitation (ChIP) and luciferase assays.ZNF677 is frequently downregulated in RCC tissues and its low expression is associated with unfavourable prognosis and decreased m6 A modification level. Further, we find the m6 A-modified coding sequence (CDS) of ZNF677 positively regulates its translation and mRNA stability via binding with YTHDF1 and IGF2BP2, respectively. Targeted specific methylation of ZNF677 m6 A by CRISPR/dCas13b-METLL3 system can significantly increase the m6 A and expression level of ZNF677, and dramatically inhibit cell proliferation and induce cell apoptosis of RCC cells. In addition, ZNF677 exerted its tumour suppressor functions in RCC cells through transcriptional repression of CDKN3 via binding to its promoter. In vitro and clinical data confirm the negative roles of ZNF677/CDKN3 in tumour growth and progression of RCC.ZNF677 functions as a tumour suppressor and is frequently silenced via m6 A modification in RCC, which may highlight m6 A methylation-based approach for RCC diagnosis and therapy." @default.
• W4281695964 created "2022-06-13" @default.
• W4281695964 creator A5009177784 @default.
• W4281695964 creator A5020919220 @default.
• W4281695964 creator A5028702225 @default.
• W4281695964 creator A5032654916 @default.
• W4281695964 creator A5034202632 @default.
• W4281695964 creator A5046212411 @default.
• W4281695964 creator A5052441498 @default.
• W4281695964 creator A5055720455 @default.
• W4281695964 creator A5079266488 @default.
• W4281695964 date "2022-06-01" @default.
• W4281695964 modified "2023-10-16" @default.
• W4281695964 title "ZNF677 suppresses renal cell carcinoma progression through N6‐methyladenosine and transcriptional repression of CDKN3" @default.
• W4281695964 cites W1024528920 @default.
• W4281695964 cites W1555039175 @default.
• W4281695964 cites W1588966097 @default.
• W4281695964 cites W1933156045 @default.
• W4281695964 cites W1941339056 @default.
• W4281695964 cites W1991807334 @default.
• W4281695964 cites W2002283210 @default.
• W4281695964 cites W2007429249 @default.
• W4281695964 cites W2009482523 @default.
• W4281695964 cites W2011131953 @default.
• W4281695964 cites W2025222882 @default.
• W4281695964 cites W2054101905 @default.
• W4281695964 cites W2065847836 @default.
• W4281695964 cites W2071930821 @default.
• W4281695964 cites W2078964320 @default.
• W4281695964 cites W2085184586 @default.
• W4281695964 cites W2126496132 @default.
• W4281695964 cites W2171808845 @default.
• W4281695964 cites W2306179365 @default.
• W4281695964 cites W2342299314 @default.
• W4281695964 cites W2542503890 @default.
• W4281695964 cites W2546967042 @default.
• W4281695964 cites W2563912688 @default.
• W4281695964 cites W2584435361 @default.
• W4281695964 cites W2591501909 @default.
• W4281695964 cites W2600626962 @default.
• W4281695964 cites W2627007286 @default.
• W4281695964 cites W2627064270 @default.
• W4281695964 cites W2767377392 @default.
• W4281695964 cites W2770747479 @default.
• W4281695964 cites W2788604588 @default.
• W4281695964 cites W2793785833 @default.
• W4281695964 cites W2800525204 @default.
• W4281695964 cites W2802358759 @default.
• W4281695964 cites W2810207915 @default.
• W4281695964 cites W2836731941 @default.
• W4281695964 cites W2888375528 @default.
• W4281695964 cites W2911188335 @default.
• W4281695964 cites W2923286987 @default.
• W4281695964 cites W2937167259 @default.
• W4281695964 cites W2942683196 @default.
• W4281695964 cites W2945068621 @default.
• W4281695964 cites W2959403793 @default.
• W4281695964 cites W2969469813 @default.
• W4281695964 cites W2977870022 @default.
• W4281695964 cites W3019798360 @default.
• W4281695964 cites W3027037948 @default.
• W4281695964 cites W3037464909 @default.
• W4281695964 cites W3135173016 @default.
• W4281695964 cites W3135755065 @default.
• W4281695964 cites W3166539655 @default.
• W4281695964 cites W3174565000 @default.
• W4281695964 cites W4281695964 @default.
• W4281695964 doi "https://doi.org/10.1002/ctm2.906" @default.
• W4281695964 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35678231" @default.
• W4281695964 hasPublicationYear "2022" @default.
• W4281695964 type Work @default.
• W4281695964 citedByCount "13" @default.
• W4281695964 countsByYear W42816959642022 @default.
• W4281695964 countsByYear W42816959642023 @default.
• W4281695964 crossrefType "journal-article" @default.
• W4281695964 hasAuthorship W4281695964A5009177784 @default.
• W4281695964 hasAuthorship W4281695964A5020919220 @default.
• W4281695964 hasAuthorship W4281695964A5028702225 @default.
• W4281695964 hasAuthorship W4281695964A5032654916 @default.
• W4281695964 hasAuthorship W4281695964A5034202632 @default.
• W4281695964 hasAuthorship W4281695964A5046212411 @default.
• W4281695964 hasAuthorship W4281695964A5052441498 @default.
• W4281695964 hasAuthorship W4281695964A5055720455 @default.
• W4281695964 hasAuthorship W4281695964A5079266488 @default.
• W4281695964 hasBestOaLocation W42816959641 @default.
• W4281695964 hasConcept C101762097 @default.
• W4281695964 hasConcept C104317684 @default.
• W4281695964 hasConcept C105580179 @default.
• W4281695964 hasConcept C134320426 @default.
• W4281695964 hasConcept C150194340 @default.
• W4281695964 hasConcept C153911025 @default.
• W4281695964 hasConcept C190727270 @default.
• W4281695964 hasConcept C24284526 @default.
• W4281695964 hasConcept C2775963939 @default.
• W4281695964 hasConcept C33288867 @default.
• W4281695964 hasConcept C502942594 @default.
• W4281695964 hasConcept C54355233 @default.
• W4281695964 hasConcept C62112901 @default.
• W4281695964 hasConcept C67705224 @default.
• W4281695964 hasConcept C71829478 @default.

• next