FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4281700900> ?p ?o ?g. }

• W4281700900 endingPage "109314" @default.
• W4281700900 startingPage "109314" @default.
• W4281700900 abstract "Phthalate cis -4,5-dihydrodiol dehydrogenase (PhtC), the second enzyme of the phthalate catabolic pathway, catalyzes the dehydrogenation of cis -4,5-dihydrodiol phthalate (DDP). Here, we report the structural and biochemical characterization of PhtC from Comamonas testosteroni KF1 (PhtC KF1 ). With biochemical experiments, we have determined the enzyme's catalytic efficiency ( k cat / K m ) with DDP as 2.6 ± 0.5 M −1 s −1 , over 10-fold higher than with cis -3,4-dihydrodiol phthalate (CDP). To understand the structural basis of these reactions, the crystal structures of PhtC KF1 in apo-form, the binary complex with NAD + , and the ternary complex with NAD + and 3-hydroxybenzoate (3HB) were determined. These crystal structures reveal that the binding of 3HB induces a conformational change in the substrate-binding loop. This conformational change causes the opening of the NAD + binding site while trapping the 3HB. The PhtC KF1 crystal structures show that the catalytic domain of PhtC KF1 is larger than that of other structurally characterized homologs and does not align with other cis -diol dehydrogenases. Structural and mutational analysis of the substrate-binding loop residues, Arg164 and Glu167 establish that conformational flexibility of this loop is necessary for positioning the substrate in a catalytically competent pose, as substitution of either of these residues to Ala did not yield the dehydrogenation activity. Further, based on the crystal structures of PhtC KF1 and related structural homologs, a reaction mechanism is proposed. Finally, with the biochemical analysis of a variant M251LPhtC KF1 , the broader substrate specificity of this enzyme is explained. • cis -4,5-dihydrodiol phthalate dehydrogenase (PhtC) catalyzes the dehydrogenation of cis -3,4-dihydrodiol phthalate as well. • PhtC structure shows distinct fold compared to other cis -diol dehydrogenases. • Structure of complex of PhtC with 3-hydroxybenzoate and NAD + and mutational analysis show conformational flexibility of substrate binding loop for catalysis. • Variant M251LPhtC has identical active site residue as cis -dihydrodiol-3-chlorobenzoate dehydrogenase." @default.
• W4281700900 created "2022-06-13" @default.
• W4281700900 creator A5003425456 @default.
• W4281700900 creator A5019129928 @default.
• W4281700900 creator A5035012142 @default.
• W4281700900 creator A5040368058 @default.
• W4281700900 creator A5047930038 @default.
• W4281700900 creator A5052838748 @default.
• W4281700900 date "2022-09-01" @default.
• W4281700900 modified "2023-09-24" @default.
• W4281700900 title "Conformational flexibility enables catalysis of phthalate cis-4,5-dihydrodiol dehydrogenase" @default.
• W4281700900 cites W1567698297 @default.
• W4281700900 cites W1857087500 @default.
• W4281700900 cites W1919611035 @default.
• W4281700900 cites W1965133779 @default.
• W4281700900 cites W1965978566 @default.
• W4281700900 cites W1973668045 @default.
• W4281700900 cites W1985579861 @default.
• W4281700900 cites W1987069639 @default.
• W4281700900 cites W1997900804 @default.
• W4281700900 cites W2005531169 @default.
• W4281700900 cites W2008000048 @default.
• W4281700900 cites W2008476781 @default.
• W4281700900 cites W2015004391 @default.
• W4281700900 cites W2015395569 @default.
• W4281700900 cites W2018735835 @default.
• W4281700900 cites W2026043808 @default.
• W4281700900 cites W2034760797 @default.
• W4281700900 cites W2035503835 @default.
• W4281700900 cites W2065022745 @default.
• W4281700900 cites W2067905803 @default.
• W4281700900 cites W2070043644 @default.
• W4281700900 cites W2094243572 @default.
• W4281700900 cites W2104148332 @default.
• W4281700900 cites W2116414322 @default.
• W4281700900 cites W2122339645 @default.
• W4281700900 cites W2132629607 @default.
• W4281700900 cites W2134967712 @default.
• W4281700900 cites W2141865076 @default.
• W4281700900 cites W2144081223 @default.
• W4281700900 cites W2152329390 @default.
• W4281700900 cites W2159211495 @default.
• W4281700900 cites W2160605328 @default.
• W4281700900 cites W2161321889 @default.
• W4281700900 cites W2168974130 @default.
• W4281700900 cites W2175273443 @default.
• W4281700900 cites W2242223387 @default.
• W4281700900 cites W2311203695 @default.
• W4281700900 cites W2324795887 @default.
• W4281700900 cites W2608840305 @default.
• W4281700900 cites W2803267022 @default.
• W4281700900 cites W2908772608 @default.
• W4281700900 cites W2938574745 @default.
• W4281700900 cites W2980170804 @default.
• W4281700900 cites W3010169717 @default.
• W4281700900 cites W3015104130 @default.
• W4281700900 cites W3086777632 @default.
• W4281700900 cites W4211196614 @default.
• W4281700900 cites W4225616420 @default.
• W4281700900 doi "https://doi.org/10.1016/j.abb.2022.109314" @default.
• W4281700900 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35667443" @default.
• W4281700900 hasPublicationYear "2022" @default.
• W4281700900 type Work @default.
• W4281700900 citedByCount "2" @default.
• W4281700900 countsByYear W42817009002023 @default.
• W4281700900 crossrefType "journal-article" @default.
• W4281700900 hasAuthorship W4281700900A5003425456 @default.
• W4281700900 hasAuthorship W4281700900A5019129928 @default.
• W4281700900 hasAuthorship W4281700900A5035012142 @default.
• W4281700900 hasAuthorship W4281700900A5040368058 @default.
• W4281700900 hasAuthorship W4281700900A5047930038 @default.
• W4281700900 hasAuthorship W4281700900A5052838748 @default.
• W4281700900 hasConcept C178790620 @default.
• W4281700900 hasConcept C181199279 @default.
• W4281700900 hasConcept C185592680 @default.
• W4281700900 hasConcept C18903297 @default.
• W4281700900 hasConcept C197957613 @default.
• W4281700900 hasConcept C2776317432 @default.
• W4281700900 hasConcept C2777289219 @default.
• W4281700900 hasConcept C2777339483 @default.
• W4281700900 hasConcept C2779268744 @default.
• W4281700900 hasConcept C2780021053 @default.
• W4281700900 hasConcept C2781005124 @default.
• W4281700900 hasConcept C41183919 @default.
• W4281700900 hasConcept C55493867 @default.
• W4281700900 hasConcept C71240020 @default.
• W4281700900 hasConcept C75520062 @default.
• W4281700900 hasConcept C86756495 @default.
• W4281700900 hasConcept C86803240 @default.
• W4281700900 hasConceptScore W4281700900C178790620 @default.
• W4281700900 hasConceptScore W4281700900C181199279 @default.
• W4281700900 hasConceptScore W4281700900C185592680 @default.
• W4281700900 hasConceptScore W4281700900C18903297 @default.
• W4281700900 hasConceptScore W4281700900C197957613 @default.
• W4281700900 hasConceptScore W4281700900C2776317432 @default.
• W4281700900 hasConceptScore W4281700900C2777289219 @default.
• W4281700900 hasConceptScore W4281700900C2777339483 @default.
• W4281700900 hasConceptScore W4281700900C2779268744 @default.

• next