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- W4281725770 abstract "High-resolution immunofluorescence imaging of cochlear hair bundles faces many challenges due to the hair bundle's small dimensions, fragile nature, and complex organization. Here, we describe an optimized protocol for hair-bundle protein immunostaining and localization. We detail the steps and solutions for extracting and fixing the mouse inner ear and for dissecting the organ of Corti. We further emphasize the optimal permeabilization, blocking, staining, and mounting conditions as well as the parameters for high-resolution microscopy imaging. For complete details on the use and execution of this protocol, please refer to Trouillet et al. (2021)." @default.
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- W4281725770 date "2022-06-01" @default.
- W4281725770 modified "2023-09-30" @default.
- W4281725770 title "High-resolution immunofluorescence imaging of mouse cochlear hair bundles" @default.
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- W4281725770 doi "https://doi.org/10.1016/j.xpro.2022.101431" @default.
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