Matches in SemOpenAlex for { <https://semopenalex.org/work/W4281736274> ?p ?o ?g. }
- W4281736274 endingPage "6422" @default.
- W4281736274 startingPage "6414" @default.
- W4281736274 abstract "CRISPR-Cas12a (Cpf1) is a bacterial RNA-guided nuclease that cuts double-stranded DNA (dsDNA) at sites specified by a CRISPR RNA (crRNA) guide. Additional activities have been ascribed to this enzyme in vitro: site-specific (cis) single-stranded DNA (ssDNA) cleavage and indiscriminate (trans) degradation of ssDNA, RNA, and dsDNA after activation by a complementary target. The ability of Cas12a to cleave nucleic acids indiscriminately has been harnessed for many applications, including diagnostics, but it remains unknown if it contributes to bacterial immunity. Here, we provide evidence that cleavage of ssDNA in cis or in trans by Cas12a is insufficient to impact immunity. Using LbCas12a expressed in either Pseudomonas aeruginosa or Escherichia coli, we observed that cleavage of dsDNA targets did not elicit cell death or dormancy, suggesting insignificant levels of collateral damage against host RNA or DNA. Canonical immunity against invasive dsDNA also had no impact on the replicative fitness of co-infecting dsDNA phage, ssDNA phage or plasmid in trans. Lastly, crRNAs complementary to invasive ssDNA did not provide protection, suggesting that ssDNA cleavage does not occur in vivo or is insignificant. Overall, these results suggest that CRISPR-Cas12a immunity predominantly occurs via canonical targeting of dsDNA, and that the other activities do not significantly impact infection outcomes." @default.
- W4281736274 created "2022-06-13" @default.
- W4281736274 creator A5004296344 @default.
- W4281736274 creator A5010734874 @default.
- W4281736274 creator A5091580278 @default.
- W4281736274 date "2022-06-07" @default.
- W4281736274 modified "2023-10-14" @default.
- W4281736274 title "CRISPR-Cas12a targeting of ssDNA plays no detectable role in immunity" @default.
- W4281736274 cites W1500856445 @default.
- W4281736274 cites W1924027056 @default.
- W4281736274 cites W2002198795 @default.
- W4281736274 cites W2011882432 @default.
- W4281736274 cites W2017266538 @default.
- W4281736274 cites W2020486543 @default.
- W4281736274 cites W2044756716 @default.
- W4281736274 cites W2048851100 @default.
- W4281736274 cites W2071717671 @default.
- W4281736274 cites W2079637221 @default.
- W4281736274 cites W2086358238 @default.
- W4281736274 cites W2165869860 @default.
- W4281736274 cites W2229444697 @default.
- W4281736274 cites W2269848625 @default.
- W4281736274 cites W2341289363 @default.
- W4281736274 cites W2341405505 @default.
- W4281736274 cites W2409671300 @default.
- W4281736274 cites W2410526873 @default.
- W4281736274 cites W2465120080 @default.
- W4281736274 cites W2519962384 @default.
- W4281736274 cites W2560230043 @default.
- W4281736274 cites W2607086226 @default.
- W4281736274 cites W2620475836 @default.
- W4281736274 cites W2751368707 @default.
- W4281736274 cites W2766596535 @default.
- W4281736274 cites W2770555788 @default.
- W4281736274 cites W2782104605 @default.
- W4281736274 cites W2787024379 @default.
- W4281736274 cites W2790313560 @default.
- W4281736274 cites W2803295557 @default.
- W4281736274 cites W2891877869 @default.
- W4281736274 cites W2902246058 @default.
- W4281736274 cites W2902678964 @default.
- W4281736274 cites W2931697891 @default.
- W4281736274 cites W2946892453 @default.
- W4281736274 cites W2950877338 @default.
- W4281736274 cites W2979828019 @default.
- W4281736274 cites W2995081665 @default.
- W4281736274 cites W2997842553 @default.
- W4281736274 cites W3000984351 @default.
- W4281736274 cites W3004706073 @default.
- W4281736274 cites W3007468112 @default.
- W4281736274 cites W3008492600 @default.
- W4281736274 cites W3012033772 @default.
- W4281736274 cites W3036640238 @default.
- W4281736274 cites W3083245676 @default.
- W4281736274 cites W3093718851 @default.
- W4281736274 cites W3112571069 @default.
- W4281736274 cites W3125886950 @default.
- W4281736274 cites W3131429738 @default.
- W4281736274 cites W3142284439 @default.
- W4281736274 cites W3148105589 @default.
- W4281736274 cites W3162126409 @default.
- W4281736274 cites W3178418858 @default.
- W4281736274 cites W3183969621 @default.
- W4281736274 cites W3198162943 @default.
- W4281736274 cites W3198740632 @default.
- W4281736274 doi "https://doi.org/10.1093/nar/gkac462" @default.
- W4281736274 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35670674" @default.
- W4281736274 hasPublicationYear "2022" @default.
- W4281736274 type Work @default.
- W4281736274 citedByCount "10" @default.
- W4281736274 countsByYear W42817362742022 @default.
- W4281736274 countsByYear W42817362742023 @default.
- W4281736274 crossrefType "journal-article" @default.
- W4281736274 hasAuthorship W4281736274A5004296344 @default.
- W4281736274 hasAuthorship W4281736274A5010734874 @default.
- W4281736274 hasAuthorship W4281736274A5091580278 @default.
- W4281736274 hasBestOaLocation W42817362741 @default.
- W4281736274 hasConcept C104317684 @default.
- W4281736274 hasConcept C132455925 @default.
- W4281736274 hasConcept C151730666 @default.
- W4281736274 hasConcept C153911025 @default.
- W4281736274 hasConcept C175156509 @default.
- W4281736274 hasConcept C22744801 @default.
- W4281736274 hasConcept C24107716 @default.
- W4281736274 hasConcept C2777271071 @default.
- W4281736274 hasConcept C2779341262 @default.
- W4281736274 hasConcept C43369102 @default.
- W4281736274 hasConcept C54355233 @default.
- W4281736274 hasConcept C552990157 @default.
- W4281736274 hasConcept C67705224 @default.
- W4281736274 hasConcept C84356384 @default.
- W4281736274 hasConcept C86803240 @default.
- W4281736274 hasConcept C8891405 @default.
- W4281736274 hasConcept C95444343 @default.
- W4281736274 hasConcept C98108389 @default.
- W4281736274 hasConceptScore W4281736274C104317684 @default.
- W4281736274 hasConceptScore W4281736274C132455925 @default.
- W4281736274 hasConceptScore W4281736274C151730666 @default.