Matches in SemOpenAlex for { <https://semopenalex.org/work/W4281736286> ?p ?o ?g. }
- W4281736286 abstract "ABSTRACT Host-controlled intracellular accommodation of nitrogen-fixing bacteria is essential for the establishment of a functional Root Nodule Symbiosis (RNS). In many host plants, this occurs via transcellular tubular-structures (infection threads - ITs) that extend across cell layers via polar tip- growth. Comparative phylogenomic studies have identified RPG (RHIZOBIUM-DIRECTED POLAR GROWTH) among the critical genetic determinants for bacterial infection. In Medicago truncatula , RPG is required for effective IT progression within root hairs but the cellular and molecular function of the encoded protein remain elusive. Here, we show that RPG resides in the protein complex formed by the core endosymbiotic components VAPYRIN (VPY) and LUMPY INFECTION (LIN) required for IT polar growth, co- localizes with both VPY and LIN in IT tip- and perinuclear-associated puncta of M. truncatula root hairs undergoing infection and is necessary for VPY recruitment to puncta. Fluorescence Lifetime Imaging Microscopy (FLIM) of phosphoinositide species during bacterial infection revealed that functional RPG is required to sustain strong membrane polarization at the advancing tip of the IT. In addition, loss of RPG functionality alters the cytoskeleton-mediated connectivity between the IT tip and the nucleus and affects polar secretion of the cell wall modifying enzyme NODULE PECTATE LYASE (NPL). Our results integrate RPG into a core host machinery required to support symbiont accommodation, suggesting that its occurrence in plant host genomes is essential to co-opt a multimeric protein module committed to endosymbiosis to sustain IT-mediated bacterial infection." @default.
- W4281736286 created "2022-06-13" @default.
- W4281736286 creator A5027328943 @default.
- W4281736286 creator A5027767588 @default.
- W4281736286 creator A5031181104 @default.
- W4281736286 creator A5040945074 @default.
- W4281736286 creator A5048328617 @default.
- W4281736286 creator A5052422800 @default.
- W4281736286 creator A5067988468 @default.
- W4281736286 creator A5071004480 @default.
- W4281736286 creator A5077136227 @default.
- W4281736286 date "2022-06-04" @default.
- W4281736286 modified "2023-10-18" @default.
- W4281736286 title "RPG acts as a central determinant for infectosome formation and cellular polarization during intracellular rhizobial infections" @default.
- W4281736286 cites W1532211930 @default.
- W4281736286 cites W1547706315 @default.
- W4281736286 cites W1626869794 @default.
- W4281736286 cites W1773424352 @default.
- W4281736286 cites W1964296810 @default.
- W4281736286 cites W1966302524 @default.
- W4281736286 cites W1970893992 @default.
- W4281736286 cites W1973138436 @default.
- W4281736286 cites W1977147232 @default.
- W4281736286 cites W1979817897 @default.
- W4281736286 cites W1991767608 @default.
- W4281736286 cites W2022037801 @default.
- W4281736286 cites W2025481379 @default.
- W4281736286 cites W2042639289 @default.
- W4281736286 cites W2042915641 @default.
- W4281736286 cites W2046737010 @default.
- W4281736286 cites W2048060135 @default.
- W4281736286 cites W2048717123 @default.
- W4281736286 cites W2054530395 @default.
- W4281736286 cites W2059818128 @default.
- W4281736286 cites W2066273886 @default.
- W4281736286 cites W2074665339 @default.
- W4281736286 cites W2091121523 @default.
- W4281736286 cites W2093282755 @default.
- W4281736286 cites W2103745952 @default.
- W4281736286 cites W2114115878 @default.
- W4281736286 cites W2115926081 @default.
- W4281736286 cites W2118771542 @default.
- W4281736286 cites W2126603410 @default.
- W4281736286 cites W2127564412 @default.
- W4281736286 cites W2127911529 @default.
- W4281736286 cites W2128255331 @default.
- W4281736286 cites W2132063003 @default.
- W4281736286 cites W2146289413 @default.
- W4281736286 cites W2149487218 @default.
- W4281736286 cites W2153451605 @default.
- W4281736286 cites W2156017482 @default.
- W4281736286 cites W2157312621 @default.
- W4281736286 cites W2160619076 @default.
- W4281736286 cites W2166371308 @default.
- W4281736286 cites W2166397221 @default.
- W4281736286 cites W2168317594 @default.
- W4281736286 cites W2175560100 @default.
- W4281736286 cites W2304624390 @default.
- W4281736286 cites W2444667442 @default.
- W4281736286 cites W2474342608 @default.
- W4281736286 cites W2491458126 @default.
- W4281736286 cites W2515844732 @default.
- W4281736286 cites W2800653759 @default.
- W4281736286 cites W2886785109 @default.
- W4281736286 cites W2889971728 @default.
- W4281736286 cites W2896196514 @default.
- W4281736286 cites W2913752596 @default.
- W4281736286 cites W2947804060 @default.
- W4281736286 cites W2981700993 @default.
- W4281736286 cites W2991321156 @default.
- W4281736286 cites W2999147930 @default.
- W4281736286 cites W3010199183 @default.
- W4281736286 cites W3011759473 @default.
- W4281736286 cites W3027586806 @default.
- W4281736286 cites W3089793279 @default.
- W4281736286 cites W3110417971 @default.
- W4281736286 cites W3133894782 @default.
- W4281736286 cites W3158864408 @default.
- W4281736286 cites W3163861868 @default.
- W4281736286 cites W4220911609 @default.
- W4281736286 doi "https://doi.org/10.1101/2022.06.03.494689" @default.
- W4281736286 hasPublicationYear "2022" @default.
- W4281736286 type Work @default.
- W4281736286 citedByCount "1" @default.
- W4281736286 countsByYear W42817362862022 @default.
- W4281736286 crossrefType "posted-content" @default.
- W4281736286 hasAuthorship W4281736286A5027328943 @default.
- W4281736286 hasAuthorship W4281736286A5027767588 @default.
- W4281736286 hasAuthorship W4281736286A5031181104 @default.
- W4281736286 hasAuthorship W4281736286A5040945074 @default.
- W4281736286 hasAuthorship W4281736286A5048328617 @default.
- W4281736286 hasAuthorship W4281736286A5052422800 @default.
- W4281736286 hasAuthorship W4281736286A5067988468 @default.
- W4281736286 hasAuthorship W4281736286A5071004480 @default.
- W4281736286 hasAuthorship W4281736286A5077136227 @default.
- W4281736286 hasBestOaLocation W42817362861 @default.
- W4281736286 hasConcept C104317684 @default.
- W4281736286 hasConcept C199733737 @default.
- W4281736286 hasConcept C2778339097 @default.
- W4281736286 hasConcept C43144210 @default.