Matches in SemOpenAlex for { <https://semopenalex.org/work/W4281738255> ?p ?o ?g. }
- W4281738255 endingPage "1" @default.
- W4281738255 startingPage "1" @default.
- W4281738255 abstract "Purpose: To investigate the expression of corneal epithelium–derived netrin-1 (NTN-1) and its immunoregulatory function in dry eye disease (DED) using a DED mouse model. Methods: We generated DED mouse models with desiccating stress under scopolamine treatment. RNA sequencing was performed to identify differentially expressed genes (DEGs) in the corneal epithelium of DED mice. NTN-1 expression was analyzed via real-time PCR, immunofluorescence staining, and immunoblotting. The DED mice were then treated with recombinant NTN-1 or neutralizing antibodies to investigate the severity of the disease, dendritic cell (DC) activation, and inflammatory cytokine expression. Results: A total of 347 DEGs (292 upregulated and 55 downregulated) were identified in the corneal epithelium of DED mice: corneal epithelium–derived NTN-1 expression was significantly decreased in DED mice compared to that in control mice. Topical recombinant NTN-1 application alleviated the severity of the disease, accompanied by restoration of tear secretion and goblet cell density. In addition, NTN-1 decreased the number of DCs, inhibited the activation of the DCs and Th17 cells, and reduced the expression of inflammatory factors in DED mice. In contrast, blocking endogenous NTN-1 activity with an anti–NTN-1 antibody aggravated the disease, enhanced DC activation, and upregulated the inflammatory factors in the conjunctivae of DED mice. Conclusions: We identified decreased NTN-1 expression in the corneal epithelium of DED mice. Our findings elucidate the role of NTN-1 in alleviating DED and impeding DC activation, thereby indicating its therapeutic potential in suppressing ocular inflammation in DED." @default.
- W4281738255 created "2022-06-13" @default.
- W4281738255 creator A5006193770 @default.
- W4281738255 creator A5015862544 @default.
- W4281738255 creator A5022009623 @default.
- W4281738255 creator A5025030643 @default.
- W4281738255 creator A5026028301 @default.
- W4281738255 creator A5033605952 @default.
- W4281738255 creator A5068568296 @default.
- W4281738255 creator A5076226909 @default.
- W4281738255 date "2022-06-01" @default.
- W4281738255 modified "2023-09-25" @default.
- W4281738255 title "Corneal Epithelium–Derived Netrin-1 Alleviates Dry Eye Disease via Regulating Dendritic Cell Activation" @default.
- W4281738255 cites W1983009944 @default.
- W4281738255 cites W1984309285 @default.
- W4281738255 cites W1992968184 @default.
- W4281738255 cites W2002180108 @default.
- W4281738255 cites W2011777137 @default.
- W4281738255 cites W2012144205 @default.
- W4281738255 cites W2020624195 @default.
- W4281738255 cites W2021028590 @default.
- W4281738255 cites W2023306844 @default.
- W4281738255 cites W2041738284 @default.
- W4281738255 cites W2046130726 @default.
- W4281738255 cites W2046968637 @default.
- W4281738255 cites W2062356301 @default.
- W4281738255 cites W2063237293 @default.
- W4281738255 cites W2074660641 @default.
- W4281738255 cites W2075406746 @default.
- W4281738255 cites W2079846656 @default.
- W4281738255 cites W2089150896 @default.
- W4281738255 cites W2105558032 @default.
- W4281738255 cites W2131109936 @default.
- W4281738255 cites W2137267666 @default.
- W4281738255 cites W2139090780 @default.
- W4281738255 cites W2156516646 @default.
- W4281738255 cites W2164655074 @default.
- W4281738255 cites W2166332777 @default.
- W4281738255 cites W2171430554 @default.
- W4281738255 cites W2547246158 @default.
- W4281738255 cites W2604324397 @default.
- W4281738255 cites W2737787547 @default.
- W4281738255 cites W27632251 @default.
- W4281738255 cites W2868111620 @default.
- W4281738255 cites W2883734051 @default.
- W4281738255 cites W2884548335 @default.
- W4281738255 cites W2891392712 @default.
- W4281738255 cites W2905700552 @default.
- W4281738255 cites W2926942314 @default.
- W4281738255 cites W2948777351 @default.
- W4281738255 cites W2969667038 @default.
- W4281738255 cites W2972732188 @default.
- W4281738255 cites W2981429543 @default.
- W4281738255 cites W3021739351 @default.
- W4281738255 cites W3030632859 @default.
- W4281738255 cites W3082834524 @default.
- W4281738255 cites W3083500597 @default.
- W4281738255 cites W3118578035 @default.
- W4281738255 cites W3124001821 @default.
- W4281738255 cites W3125314599 @default.
- W4281738255 cites W3131026858 @default.
- W4281738255 cites W3150594469 @default.
- W4281738255 cites W3171676404 @default.
- W4281738255 cites W3195282723 @default.
- W4281738255 cites W3200744402 @default.
- W4281738255 cites W4251663120 @default.
- W4281738255 cites W884116207 @default.
- W4281738255 doi "https://doi.org/10.1167/iovs.63.6.1" @default.
- W4281738255 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35648640" @default.
- W4281738255 hasPublicationYear "2022" @default.
- W4281738255 type Work @default.
- W4281738255 citedByCount "4" @default.
- W4281738255 countsByYear W42817382552022 @default.
- W4281738255 countsByYear W42817382552023 @default.
- W4281738255 crossrefType "journal-article" @default.
- W4281738255 hasAuthorship W4281738255A5006193770 @default.
- W4281738255 hasAuthorship W4281738255A5015862544 @default.
- W4281738255 hasAuthorship W4281738255A5022009623 @default.
- W4281738255 hasAuthorship W4281738255A5025030643 @default.
- W4281738255 hasAuthorship W4281738255A5026028301 @default.
- W4281738255 hasAuthorship W4281738255A5033605952 @default.
- W4281738255 hasAuthorship W4281738255A5068568296 @default.
- W4281738255 hasAuthorship W4281738255A5076226909 @default.
- W4281738255 hasBestOaLocation W42817382551 @default.
- W4281738255 hasConcept C104317684 @default.
- W4281738255 hasConcept C127561419 @default.
- W4281738255 hasConcept C142724271 @default.
- W4281738255 hasConcept C153911025 @default.
- W4281738255 hasConcept C203014093 @default.
- W4281738255 hasConcept C2776914184 @default.
- W4281738255 hasConcept C2778170410 @default.
- W4281738255 hasConcept C2779257441 @default.
- W4281738255 hasConcept C529295009 @default.
- W4281738255 hasConcept C55493867 @default.
- W4281738255 hasConcept C71924100 @default.
- W4281738255 hasConcept C86803240 @default.
- W4281738255 hasConcept C8891405 @default.
- W4281738255 hasConcept C95444343 @default.