FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4281743812> ?p ?o ?g. }

• W4281743812 abstract "Promiscuous activity of the Streptococcus pyogenes DNA nuclease CRISPR-Cas9 can result in destruction of a successfully modified sequence obtained by templated repair of a Cas9-induced DNA double-strand break. To avoid re-cutting, additional target-site-disruptions (TSDs) are often introduced on top of the desired base-pair alteration in order to suppress target recognition. These TSDs may lower the efficiency of introducing the intended mutation and can cause unexpected phenotypes. Alternatively, successfully edited sites can be protected against Cas9 re-cutting activity. This method exploits the finding that Cas9 complexed to trimmed guideRNAs can still tightly bind specific genomic sequences but lacks nuclease activity. We show here that the presence of a guideRNA plus a trimmed guideRNA that matches the successfully mutated sequence, which we call hideRNA, can enhance the recovery of precise single base-pair substitution events tenfold. The benefit of hideRNAs in generating a single point mutation was demonstrated in cell lines using plasmid-based delivery of CRISPR-Cas9 components and in mouse zygotes injected with Cas9/guideRNA plus Cas9/hideRNA ribonucleoprotein complexes. However, hRNA protection sometimes failed, which likely reflects an unfavorable affinity of hRNA/Cas9 versus gRNA/Cas9 for the DNA target site. HideRNAs can easily be implemented into current gene editing protocols and facilitate the recovery of single base-pair substitution. As such, hideRNAs are of great value in gene editing experiments demanding high accuracy." @default.
• W4281743812 created "2022-06-13" @default.
• W4281743812 creator A5002551113 @default.
• W4281743812 creator A5029568628 @default.
• W4281743812 creator A5044428638 @default.
• W4281743812 creator A5046368045 @default.
• W4281743812 creator A5060096896 @default.
• W4281743812 creator A5089641684 @default.
• W4281743812 date "2022-06-10" @default.
• W4281743812 modified "2023-10-01" @default.
• W4281743812 title "HideRNAs protect against CRISPR-Cas9 re-cutting after successful single base-pair gene editing" @default.
• W4281743812 cites W1751510803 @default.
• W4281743812 cites W1970196890 @default.
• W4281743812 cites W1970617888 @default.
• W4281743812 cites W1988101725 @default.
• W4281743812 cites W2000046950 @default.
• W4281743812 cites W2003171404 @default.
• W4281743812 cites W2003797386 @default.
• W4281743812 cites W2019670881 @default.
• W4281743812 cites W2030994161 @default.
• W4281743812 cites W2036176224 @default.
• W4281743812 cites W2045435533 @default.
• W4281743812 cites W2064815984 @default.
• W4281743812 cites W2073217307 @default.
• W4281743812 cites W2077381938 @default.
• W4281743812 cites W2077659966 @default.
• W4281743812 cites W2104820165 @default.
• W4281743812 cites W2108038398 @default.
• W4281743812 cites W2110058183 @default.
• W4281743812 cites W2116534141 @default.
• W4281743812 cites W2126325519 @default.
• W4281743812 cites W2142515819 @default.
• W4281743812 cites W2149402050 @default.
• W4281743812 cites W2156319424 @default.
• W4281743812 cites W2160051211 @default.
• W4281743812 cites W2167380395 @default.
• W4281743812 cites W2188223075 @default.
• W4281743812 cites W2210357377 @default.
• W4281743812 cites W2224398381 @default.
• W4281743812 cites W2461013690 @default.
• W4281743812 cites W2542621333 @default.
• W4281743812 cites W2726053863 @default.
• W4281743812 cites W2746994345 @default.
• W4281743812 cites W2770138328 @default.
• W4281743812 cites W2792407190 @default.
• W4281743812 cites W2793309516 @default.
• W4281743812 cites W2897295193 @default.
• W4281743812 cites W2952346338 @default.
• W4281743812 cites W2953019923 @default.
• W4281743812 cites W2981137429 @default.
• W4281743812 cites W3030181293 @default.
• W4281743812 cites W3049623558 @default.
• W4281743812 doi "https://doi.org/10.1038/s41598-022-13688-y" @default.
• W4281743812 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35688932" @default.
• W4281743812 hasPublicationYear "2022" @default.
• W4281743812 type Work @default.
• W4281743812 citedByCount "0" @default.
• W4281743812 crossrefType "journal-article" @default.
• W4281743812 hasAuthorship W4281743812A5002551113 @default.
• W4281743812 hasAuthorship W4281743812A5029568628 @default.
• W4281743812 hasAuthorship W4281743812A5044428638 @default.
• W4281743812 hasAuthorship W4281743812A5046368045 @default.
• W4281743812 hasAuthorship W4281743812A5060096896 @default.
• W4281743812 hasAuthorship W4281743812A5089641684 @default.
• W4281743812 hasBestOaLocation W42817438121 @default.
• W4281743812 hasConcept C104317684 @default.
• W4281743812 hasConcept C132455925 @default.
• W4281743812 hasConcept C144501496 @default.
• W4281743812 hasConcept C176944494 @default.
• W4281743812 hasConcept C185581394 @default.
• W4281743812 hasConcept C22744801 @default.
• W4281743812 hasConcept C2777271071 @default.
• W4281743812 hasConcept C501734568 @default.
• W4281743812 hasConcept C54355233 @default.
• W4281743812 hasConcept C552990157 @default.
• W4281743812 hasConcept C70721500 @default.
• W4281743812 hasConcept C86803240 @default.
• W4281743812 hasConcept C97702854 @default.
• W4281743812 hasConcept C98108389 @default.
• W4281743812 hasConceptScore W4281743812C104317684 @default.
• W4281743812 hasConceptScore W4281743812C132455925 @default.
• W4281743812 hasConceptScore W4281743812C144501496 @default.
• W4281743812 hasConceptScore W4281743812C176944494 @default.
• W4281743812 hasConceptScore W4281743812C185581394 @default.
• W4281743812 hasConceptScore W4281743812C22744801 @default.
• W4281743812 hasConceptScore W4281743812C2777271071 @default.
• W4281743812 hasConceptScore W4281743812C501734568 @default.
• W4281743812 hasConceptScore W4281743812C54355233 @default.
• W4281743812 hasConceptScore W4281743812C552990157 @default.
• W4281743812 hasConceptScore W4281743812C70721500 @default.
• W4281743812 hasConceptScore W4281743812C86803240 @default.
• W4281743812 hasConceptScore W4281743812C97702854 @default.
• W4281743812 hasConceptScore W4281743812C98108389 @default.
• W4281743812 hasFunder F4320321800 @default.
• W4281743812 hasFunder F4320328233 @default.
• W4281743812 hasIssue "1" @default.
• W4281743812 hasLocation W42817438121 @default.
• W4281743812 hasLocation W42817438122 @default.
• W4281743812 hasLocation W42817438123 @default.
• W4281743812 hasOpenAccess W4281743812 @default.

• next