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• W4281749504 abstract "Eukaryotic genome DNA is wrapped around core histones and forms a nucleosome structure. Together with associated proteins and RNAs, these nucleosomes are organized three-dimensionally in the cell as chromatin. Emerging evidence demonstrates that chromatin consists of rather irregular and variable nucleosome arrangements without the regular fiber structure and that its dynamic behavior plays a critical role in regulating various genome functions. Single-nucleosome imaging is a promising method to investigate chromatin behavior in living cells. It reveals local chromatin motion, which reflects chromatin organization not observed in chemically fixed cells. The motion data is like a gold mine. Data analyses from many aspects bring us more and more information that contributes to better understanding of genome functions. In this review article, we describe imaging of single-nucleosomes and their tracked behavior through oblique illumination microscopy. We also discuss applications of this technique, especially in elucidating nucleolar organization in living cells." @default.
• W4281749504 created "2022-06-13" @default.
• W4281749504 creator A5035675072 @default.
• W4281749504 creator A5039511021 @default.
• W4281749504 creator A5077728722 @default.
• W4281749504 date "2022-06-03" @default.
• W4281749504 modified "2023-10-11" @default.
• W4281749504 title "Chromatin behavior in living cells: Lessons from single‐nucleosome imaging and tracking" @default.
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