Matches in SemOpenAlex for { <https://semopenalex.org/work/W4281750773> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W4281750773 endingPage "166" @default.
- W4281750773 startingPage "160" @default.
- W4281750773 abstract "SUMMARY Okinawa mozuku, Cladosiphon okamuranus , is one of many edible brown algae, yielding 22 Kton annually and contributing ¥5 billion to the Japanese economy. The life cycle of C. okamuranus is complicated, since the alga has self‐cloning life cycles in both haploid (N, male and female) and diploid (2N) conditions, but only diploid “seeds” (germlings) become edible sporophytes. Because haploid and diploid germlings are morphologically indistinguishable, haploid germlings are often mistakenly combined with diploid germlings for cultivation, which results in less efficient harvesting of mozuku. Sexual identification of haploid germlings is essential to develop better diploid strains by crossbreeding. With this aim, we performed RNA‐seq analysis of haploid germlings of C. okamuranus . Using its decoded diploid genome and transcriptomic information, we identified 269 genes that are expressed specifically in male or female haploids. BLAST analysis with Ectocarpus siliculosus gene models revealed that nine of 269 genes were putative sex determination‐related genes of C. okamuranus . A unique set of polymerase chain reaction primers for these nine genes was designed, and DNA amplification using primers enabled us to distinguish male and female haploid and diploid germlings. This tool will enable mozuku farmers to select diploid germlings free of haploid germlings. Using this DNA marker technique, the amount of mozuku cultivated in Okinawa is expected to increase." @default.
- W4281750773 created "2022-06-13" @default.
- W4281750773 creator A5005315468 @default.
- W4281750773 creator A5048489865 @default.
- W4281750773 creator A5076040804 @default.
- W4281750773 creator A5081284588 @default.
- W4281750773 date "2022-06-08" @default.
- W4281750773 modified "2023-09-27" @default.
- W4281750773 title "Development of <scp>DNA</scp> markers that distinguish male and female haploid germlings of the brown alga, <scp> <i>Cladosiphon okamuranus</i> </scp>" @default.
- W4281750773 cites W2069094887 @default.
- W4281750773 cites W2089712190 @default.
- W4281750773 cites W2120408923 @default.
- W4281750773 cites W2131271579 @default.
- W4281750773 cites W2141147099 @default.
- W4281750773 cites W2142678478 @default.
- W4281750773 cites W2515821160 @default.
- W4281750773 cites W2550865805 @default.
- W4281750773 cites W2592811885 @default.
- W4281750773 cites W2621543848 @default.
- W4281750773 cites W2762638728 @default.
- W4281750773 cites W2764271756 @default.
- W4281750773 cites W3037260512 @default.
- W4281750773 cites W3042062518 @default.
- W4281750773 cites W3123918778 @default.
- W4281750773 doi "https://doi.org/10.1111/pre.12489" @default.
- W4281750773 hasPublicationYear "2022" @default.
- W4281750773 type Work @default.
- W4281750773 citedByCount "1" @default.
- W4281750773 countsByYear W42817507732023 @default.
- W4281750773 crossrefType "journal-article" @default.
- W4281750773 hasAuthorship W4281750773A5005315468 @default.
- W4281750773 hasAuthorship W4281750773A5048489865 @default.
- W4281750773 hasAuthorship W4281750773A5076040804 @default.
- W4281750773 hasAuthorship W4281750773A5081284588 @default.
- W4281750773 hasBestOaLocation W42817507732 @default.
- W4281750773 hasConcept C104317684 @default.
- W4281750773 hasConcept C121050878 @default.
- W4281750773 hasConcept C143191323 @default.
- W4281750773 hasConcept C148885314 @default.
- W4281750773 hasConcept C181485312 @default.
- W4281750773 hasConcept C199360897 @default.
- W4281750773 hasConcept C2780618852 @default.
- W4281750773 hasConcept C41008148 @default.
- W4281750773 hasConcept C522721590 @default.
- W4281750773 hasConcept C54355233 @default.
- W4281750773 hasConcept C559758991 @default.
- W4281750773 hasConcept C59822182 @default.
- W4281750773 hasConcept C86803240 @default.
- W4281750773 hasConceptScore W4281750773C104317684 @default.
- W4281750773 hasConceptScore W4281750773C121050878 @default.
- W4281750773 hasConceptScore W4281750773C143191323 @default.
- W4281750773 hasConceptScore W4281750773C148885314 @default.
- W4281750773 hasConceptScore W4281750773C181485312 @default.
- W4281750773 hasConceptScore W4281750773C199360897 @default.
- W4281750773 hasConceptScore W4281750773C2780618852 @default.
- W4281750773 hasConceptScore W4281750773C41008148 @default.
- W4281750773 hasConceptScore W4281750773C522721590 @default.
- W4281750773 hasConceptScore W4281750773C54355233 @default.
- W4281750773 hasConceptScore W4281750773C559758991 @default.
- W4281750773 hasConceptScore W4281750773C59822182 @default.
- W4281750773 hasConceptScore W4281750773C86803240 @default.
- W4281750773 hasFunder F4320317111 @default.
- W4281750773 hasIssue "3" @default.
- W4281750773 hasLocation W42817507731 @default.
- W4281750773 hasLocation W42817507732 @default.
- W4281750773 hasOpenAccess W4281750773 @default.
- W4281750773 hasPrimaryLocation W42817507731 @default.
- W4281750773 hasRelatedWork W1897641335 @default.
- W4281750773 hasRelatedWork W1967246501 @default.
- W4281750773 hasRelatedWork W2052387016 @default.
- W4281750773 hasRelatedWork W2074578490 @default.
- W4281750773 hasRelatedWork W2075799221 @default.
- W4281750773 hasRelatedWork W2537456546 @default.
- W4281750773 hasRelatedWork W2581017101 @default.
- W4281750773 hasRelatedWork W31314798 @default.
- W4281750773 hasRelatedWork W4383901077 @default.
- W4281750773 hasRelatedWork W64195814 @default.
- W4281750773 hasVolume "70" @default.
- W4281750773 isParatext "false" @default.
- W4281750773 isRetracted "false" @default.
- W4281750773 workType "article" @default.