Matches in SemOpenAlex for { <https://semopenalex.org/work/W4281760134> ?p ?o ?g. }
- W4281760134 abstract "Article9 June 2022 Transparent process Cyprinid-specific duplicated membrane TLR5 senses dsRNA as functional homodimeric receptors Zhiwei Liao Zhiwei Liao orcid.org/0000-0001-6643-9495 Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, China Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, Qingdao, China Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China Contribution: Conceptualization, Software, Formal analysis, Validation, Investigation, Visualization, Methodology, Writing - original draft Search for more papers by this author Chunrong Yang Chunrong Yang College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China Contribution: Formal analysis, Writing - review & editing Search for more papers by this author Rui Jiang Rui Jiang Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, China Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, Qingdao, China Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China Contribution: Software Search for more papers by this author Wentao Zhu Wentao Zhu Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, China Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, Qingdao, China Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China Contribution: Resources Search for more papers by this author Yongan Zhang Yongan Zhang orcid.org/0000-0002-9956-3879 Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, China Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, Qingdao, China Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China Contribution: Methodology, Writing - review & editing Search for more papers by this author Jianguo Su Corresponding Author Jianguo Su [email protected] orcid.org/0000-0002-9083-0715 Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, China Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, Qingdao, China Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China Contribution: Conceptualization, Resources, Formal analysis, Investigation, Methodology, Writing - original draft, Project administration, Writing - review & editing Search for more papers by this author Zhiwei Liao Zhiwei Liao orcid.org/0000-0001-6643-9495 Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, China Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, Qingdao, China Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China Contribution: Conceptualization, Software, Formal analysis, Validation, Investigation, Visualization, Methodology, Writing - original draft Search for more papers by this author Chunrong Yang Chunrong Yang College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China Contribution: Formal analysis, Writing - review & editing Search for more papers by this author Rui Jiang Rui Jiang Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, China Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, Qingdao, China Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China Contribution: Software Search for more papers by this author Wentao Zhu Wentao Zhu Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, China Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, Qingdao, China Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China Contribution: Resources Search for more papers by this author Yongan Zhang Yongan Zhang orcid.org/0000-0002-9956-3879 Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, China Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, Qingdao, China Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China Contribution: Methodology, Writing - review & editing Search for more papers by this author Jianguo Su Corresponding Author Jianguo Su [email protected] orcid.org/0000-0002-9083-0715 Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, China Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, Qingdao, China Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China Contribution: Conceptualization, Resources, Formal analysis, Investigation, Methodology, Writing - original draft, Project administration, Writing - review & editing Search for more papers by this author Author Information Zhiwei Liao1,2,3, Chunrong Yang4, Rui Jiang1,2,3, Wentao Zhu1,2,3, Yongan Zhang1,2,3 and Jianguo Su *,1,2,3 1Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, China 2Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, Qingdao, China 3Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China 4College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China *Corresponding author. Tel: +86-27-87282227; E-mail: [email protected] EMBO Reports (2022)23:e54281https://doi.org/10.15252/embr.202154281 See also: ANR Weber (August 2022) Full textView the full text of the articlePDFDownload PDF of article text and main figures. Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions Figures & Info Abstract Membrane-embedded Toll-like receptor 5 (TLR5) functions as a homodimer to detect bacterial flagellin. Cyprinid grass carp (Ctenopharyngodon idella) encodes two TLR5 genes, CiTLR5a and CiTLR5b. Here, we show that cyprinid TLR5a and TLR5b homodimers unexpectedly bind the dsRNA analog poly(I:C) and regulate interferon (IFN) response in early endosomes and lysosomes. Although TLR5 homodimers also bind flagellin, an immune response to flagellin is only triggered by TLR5a/b heterodimer. Moreover, we demonstrate that two TLR5 paralogs have opposite effects on antiviral response: CiTLR5a slightly promotes and powerfully maintains, whereas CiTLR5b remarkably inhibits virus replication. We show that the ectodomain of CiTLR5 is required for dsRNA-induced IFN signaling, and we map the key poly(I:C) binding sites to G240 for CiTLR5a and to N547 for CiTLR5b. Furthermore, we reveal that differential N-glycosylation of CiTLR5a/b affects dsRNA-IFN signaling but has no role in flagellin-mediated NF-κB induction, with paralog-specific roles for CiTLR5a-T101 and corresponding CiTLR5b-I99. Moreover, we provide evidence that the ability to sense dsRNA represents a neofunctionalization specific for membrane-bound TLR5 in cyprinid, bridging viral and bacterial immune responses. Synopsis In the cyprinid grass carp TLR5 proteins are involved in sensing both, bacterial flagellin and viral dsRNA. TLR5a and TLR5b homodimers bind poly(I:C) and flagellin, but only poly(I:C) induces an IFN response. Flagellin-induced cytokine expression requires TLR5a-TLR5b heterodimers. Cyprinid (carp)-specific duplicated membrane TLR5 bridges viral and bacterial immune responses via homodimers and heterodimer, respectively. CiTLR5a homodimers slightly enhance and maintain viral replication while CiTLR5b homodimers induce a strong antiviral response. CiTLR5a-G240 and CiTLR5b-N547 are key dsRNA binding sites based on mutagenesis. N-glycosylation at CiTLR5a-T101 and at CiTLR5b-I99 is required for the paralog-specific interferon response, but not for ligand binding. Previous ArticleNext Article Read MoreAbout the coverClose modalView large imageVolume 23,Issue 8,03 August 2022This month's cover highlights the article ALS ‐linked KIF5A ΔExon27 mutant causes neuronal toxicity through gain‐of‐function by Devesh C. Pant, Janani Parameswaran, Jie Jiang and colleagues. The cover shows that the wild‐type kinesin motor protein KIF5A (pink ant) is in an inactive/resting state in the neuron's soma and doesn't walk along microtubules (tree branches). It becomes active and walks on microtubules when carrying cargoes such as RNA binding proteins (RBP) and mitochondria. On the other hand, the ALS‐associated mutant kinesin protein (Δ27, orange ant) is constitutively.(Cover concept by Janani Parameswaran and Devesh C. Pant. Cover illustration by DrawImpacts ( https://www.drawimpacts.com .) Volume 23Issue 83 August 2022In this issue ReferencesRelatedDetailsLoading ..." @default.
- W4281760134 created "2022-06-13" @default.
- W4281760134 creator A5031047222 @default.
- W4281760134 creator A5054436075 @default.
- W4281760134 creator A5067581467 @default.
- W4281760134 creator A5071996519 @default.
- W4281760134 creator A5083527243 @default.
- W4281760134 creator A5090529271 @default.
- W4281760134 date "2022-06-09" @default.
- W4281760134 modified "2023-10-10" @default.
- W4281760134 title "Cyprinid‐specific duplicated membrane TLR5 senses dsRNA as functional homodimeric receptors" @default.
- W4281760134 cites W1513563151 @default.
- W4281760134 cites W1514540900 @default.
- W4281760134 cites W1519633296 @default.
- W4281760134 cites W1655860314 @default.
- W4281760134 cites W1733534154 @default.
- W4281760134 cites W1811883818 @default.
- W4281760134 cites W1965642537 @default.
- W4281760134 cites W1972178744 @default.
- W4281760134 cites W1972776583 @default.
- W4281760134 cites W1983959087 @default.
- W4281760134 cites W1986516584 @default.
- W4281760134 cites W1991236592 @default.
- W4281760134 cites W1996736315 @default.
- W4281760134 cites W2001747259 @default.
- W4281760134 cites W2001757395 @default.
- W4281760134 cites W2019077744 @default.
- W4281760134 cites W2019645605 @default.
- W4281760134 cites W2021801225 @default.
- W4281760134 cites W2026884617 @default.
- W4281760134 cites W2038700638 @default.
- W4281760134 cites W2040112602 @default.
- W4281760134 cites W2042002366 @default.
- W4281760134 cites W2052206043 @default.
- W4281760134 cites W2057652201 @default.
- W4281760134 cites W2062267695 @default.
- W4281760134 cites W2064912476 @default.
- W4281760134 cites W2068359419 @default.
- W4281760134 cites W2076361525 @default.
- W4281760134 cites W2087368694 @default.
- W4281760134 cites W2094045593 @default.
- W4281760134 cites W2098855274 @default.
- W4281760134 cites W2105668062 @default.
- W4281760134 cites W2107315506 @default.
- W4281760134 cites W2122550126 @default.
- W4281760134 cites W2123335694 @default.
- W4281760134 cites W2127934565 @default.
- W4281760134 cites W2129961630 @default.
- W4281760134 cites W2130501069 @default.
- W4281760134 cites W2134967712 @default.
- W4281760134 cites W2150736048 @default.
- W4281760134 cites W2157129863 @default.
- W4281760134 cites W2162596168 @default.
- W4281760134 cites W2164866041 @default.
- W4281760134 cites W2227508145 @default.
- W4281760134 cites W2346168596 @default.
- W4281760134 cites W2486345949 @default.
- W4281760134 cites W2530273804 @default.
- W4281760134 cites W2572538813 @default.
- W4281760134 cites W2619800871 @default.
- W4281760134 cites W2772604519 @default.
- W4281760134 cites W2790813444 @default.
- W4281760134 cites W2801732185 @default.
- W4281760134 cites W2809388109 @default.
- W4281760134 cites W2890349859 @default.
- W4281760134 cites W2980687251 @default.
- W4281760134 cites W2989810482 @default.
- W4281760134 cites W2998789609 @default.
- W4281760134 cites W3000512554 @default.
- W4281760134 cites W3089422717 @default.
- W4281760134 cites W3160669632 @default.
- W4281760134 cites W3163314916 @default.
- W4281760134 cites W3217549866 @default.
- W4281760134 cites W4281760134 @default.
- W4281760134 doi "https://doi.org/10.15252/embr.202154281" @default.
- W4281760134 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35678424" @default.
- W4281760134 hasPublicationYear "2022" @default.
- W4281760134 type Work @default.
- W4281760134 citedByCount "8" @default.
- W4281760134 countsByYear W42817601342022 @default.
- W4281760134 countsByYear W42817601342023 @default.
- W4281760134 crossrefType "journal-article" @default.
- W4281760134 hasAuthorship W4281760134A5031047222 @default.
- W4281760134 hasAuthorship W4281760134A5054436075 @default.
- W4281760134 hasAuthorship W4281760134A5067581467 @default.
- W4281760134 hasAuthorship W4281760134A5071996519 @default.
- W4281760134 hasAuthorship W4281760134A5083527243 @default.
- W4281760134 hasAuthorship W4281760134A5090529271 @default.
- W4281760134 hasConcept C136449434 @default.
- W4281760134 hasConcept C170493617 @default.
- W4281760134 hasConcept C2776709828 @default.
- W4281760134 hasConcept C2777338127 @default.
- W4281760134 hasConcept C55493867 @default.
- W4281760134 hasConcept C86803240 @default.
- W4281760134 hasConcept C95444343 @default.
- W4281760134 hasConceptScore W4281760134C136449434 @default.
- W4281760134 hasConceptScore W4281760134C170493617 @default.
- W4281760134 hasConceptScore W4281760134C2776709828 @default.
- W4281760134 hasConceptScore W4281760134C2777338127 @default.
- W4281760134 hasConceptScore W4281760134C55493867 @default.