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• W4281765455 startingPage "55" @default.
• W4281765455 abstract "Thousands of genes are expressed during spermatogenesis and male infertility has a strong genetic component. Within this study, we focus on the role of Zfr2 in male fertility, a gene previously implicated in human male fertility. To date, very little is known about the role of ZFR2 in either humans or mice. To this end, the requirement for ZFR2 in male fertility was assessed using a knockout mouse model.Zfr2 was found to be expressed in the testes of both humans and mice. Deletion of Zfr2 was achieved via removal of exon 2 using CRISPR-Cas9 methods. The absence of Zfr2 did not result in a reduction in any fertility parameters assessed. Knockout males were capable of fostering litter sizes equal to wild type males, and there were no effects of Zfr2 knockout on sperm number or motility. We note Zfr2 knockout females were also fertile.The absence of Zfr2 alone is not sufficient to cause a reduction in male fertility in mice." @default.
• W4281765455 created "2022-06-13" @default.
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• W4281765455 date "2022-09-01" @default.
• W4281765455 modified "2023-10-03" @default.
• W4281765455 title "Zinc finger RNA binding protein 2 (ZFR2) is not required for male fertility in the mouse" @default.
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• W4281765455 doi "https://doi.org/10.1016/j.ydbio.2022.05.020" @default.
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