FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4281789208> ?p ?o ?g. }

• W4281789208 abstract "Abstract A diverse collection of viral pathogens target airway epithelial cells for infection, with effects ranging from mild upper respiratory tract symptoms to death of the infected individual. Among these pathogens are recently discovered and/or emergent viruses that sometimes fail to infect commonly used, immortalized cell lines and for which infection phenotypes in the respiratory tract remain unknown. Human airway epithelial cultures have been developed over the past several decades and have proven to be a useful model system in culturing hard‐to‐grow viruses and assaying various features of infection in a physiologically relevant setting. This article includes methods for the generation of well‐differentiated human airway epithelial cell cultures at air‐liquid interface that recapitulate the mucosal epithelium of the trachea/bronchus in vivo . We further detail inoculation of these cultures with respiratory viruses—specifically rhinovirus, influenza virus, and severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2)—and provide a protocol for the detection of double‐stranded RNA or viral antigen–positive cells by immunofluorescence microscopy. These techniques, together with a post‐imaging analysis, can be applied to characterize the efficiency of infection and kinetics of spread within the airway epithelium. Furthermore, these methods can be utilized in conjunction with antibodies against cellular targets to determine cell tropism and colocalization with specific host factors during infection. © 2022 The Authors. Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1 : Generation of human airway epithelial cultures at air‐liquid interface (HAE‐ALI) Basic Protocol 2 : Viral inoculation of HAE‐ALI Basic Protocol 3 : Immunofluorescence (IF)‐based detection of infected cells in HAE‐ALI" @default.
• W4281789208 created "2022-06-13" @default.
• W4281789208 creator A5018178848 @default.
• W4281789208 creator A5018254763 @default.
• W4281789208 creator A5081951584 @default.
• W4281789208 creator A5082666688 @default.
• W4281789208 creator A5085830487 @default.
• W4281789208 date "2022-06-01" @default.
• W4281789208 modified "2023-10-15" @default.
• W4281789208 title "Immunofluorescence‐Mediated Detection of Respiratory Virus Infections in Human Airway Epithelial Cultures" @default.
• W4281789208 cites W1494978858 @default.
• W4281789208 cites W1905742210 @default.
• W4281789208 cites W1982396831 @default.
• W4281789208 cites W1988423668 @default.
• W4281789208 cites W2089627898 @default.
• W4281789208 cites W2108358599 @default.
• W4281789208 cites W2130318026 @default.
• W4281789208 cites W2136461776 @default.
• W4281789208 cites W2146142464 @default.
• W4281789208 cites W2151791597 @default.
• W4281789208 cites W2154555351 @default.
• W4281789208 cites W2167279371 @default.
• W4281789208 cites W2237694531 @default.
• W4281789208 cites W2903201721 @default.
• W4281789208 cites W2914570682 @default.
• W4281789208 cites W3030748957 @default.
• W4281789208 cites W3087341089 @default.
• W4281789208 cites W3096665612 @default.
• W4281789208 cites W3112157318 @default.
• W4281789208 cites W3130163626 @default.
• W4281789208 cites W3145658842 @default.
• W4281789208 cites W3160793930 @default.
• W4281789208 cites W3165837442 @default.
• W4281789208 cites W3194096654 @default.
• W4281789208 cites W4226096259 @default.
• W4281789208 cites W4281789208 @default.
• W4281789208 doi "https://doi.org/10.1002/cpz1.453" @default.
• W4281789208 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35671174" @default.
• W4281789208 hasPublicationYear "2022" @default.
• W4281789208 type Work @default.
• W4281789208 citedByCount "3" @default.
• W4281789208 countsByYear W42817892082022 @default.
• W4281789208 countsByYear W42817892082023 @default.
• W4281789208 crossrefType "journal-article" @default.
• W4281789208 hasAuthorship W4281789208A5018178848 @default.
• W4281789208 hasAuthorship W4281789208A5018254763 @default.
• W4281789208 hasAuthorship W4281789208A5081951584 @default.
• W4281789208 hasAuthorship W4281789208A5082666688 @default.
• W4281789208 hasAuthorship W4281789208A5085830487 @default.
• W4281789208 hasBestOaLocation W42817892082 @default.
• W4281789208 hasConcept C105702510 @default.
• W4281789208 hasConcept C105922876 @default.
• W4281789208 hasConcept C126322002 @default.
• W4281789208 hasConcept C140704245 @default.
• W4281789208 hasConcept C141071460 @default.
• W4281789208 hasConcept C159047783 @default.
• W4281789208 hasConcept C159654299 @default.
• W4281789208 hasConcept C203014093 @default.
• W4281789208 hasConcept C2522874641 @default.
• W4281789208 hasConcept C2776178081 @default.
• W4281789208 hasConcept C2777714996 @default.
• W4281789208 hasConcept C2778236600 @default.
• W4281789208 hasConcept C2779278377 @default.
• W4281789208 hasConcept C2779644171 @default.
• W4281789208 hasConcept C2780314925 @default.
• W4281789208 hasConcept C2910059436 @default.
• W4281789208 hasConcept C529295009 @default.
• W4281789208 hasConcept C534529494 @default.
• W4281789208 hasConcept C54355233 @default.
• W4281789208 hasConcept C58475186 @default.
• W4281789208 hasConcept C71924100 @default.
• W4281789208 hasConcept C81885089 @default.
• W4281789208 hasConcept C86803240 @default.
• W4281789208 hasConcept C87568996 @default.
• W4281789208 hasConceptScore W4281789208C105702510 @default.
• W4281789208 hasConceptScore W4281789208C105922876 @default.
• W4281789208 hasConceptScore W4281789208C126322002 @default.
• W4281789208 hasConceptScore W4281789208C140704245 @default.
• W4281789208 hasConceptScore W4281789208C141071460 @default.
• W4281789208 hasConceptScore W4281789208C159047783 @default.
• W4281789208 hasConceptScore W4281789208C159654299 @default.
• W4281789208 hasConceptScore W4281789208C203014093 @default.
• W4281789208 hasConceptScore W4281789208C2522874641 @default.
• W4281789208 hasConceptScore W4281789208C2776178081 @default.
• W4281789208 hasConceptScore W4281789208C2777714996 @default.
• W4281789208 hasConceptScore W4281789208C2778236600 @default.
• W4281789208 hasConceptScore W4281789208C2779278377 @default.
• W4281789208 hasConceptScore W4281789208C2779644171 @default.
• W4281789208 hasConceptScore W4281789208C2780314925 @default.
• W4281789208 hasConceptScore W4281789208C2910059436 @default.
• W4281789208 hasConceptScore W4281789208C529295009 @default.
• W4281789208 hasConceptScore W4281789208C534529494 @default.
• W4281789208 hasConceptScore W4281789208C54355233 @default.
• W4281789208 hasConceptScore W4281789208C58475186 @default.
• W4281789208 hasConceptScore W4281789208C71924100 @default.
• W4281789208 hasConceptScore W4281789208C81885089 @default.
• W4281789208 hasConceptScore W4281789208C86803240 @default.
• W4281789208 hasConceptScore W4281789208C87568996 @default.
• W4281789208 hasFunder F4320337338 @default.
• W4281789208 hasFunder F4320337355 @default.

• next