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- W4281853246 abstract "Cellular mechanophenotype is often a defining characteristic of conditions like cancer malignancy/metastasis, cardiovascular disease, lung and liver fibrosis, and stem cell differentiation. However, acquiring living cells based on mechanophenotype is challenging for conventional cell sorters due to a lack of biomarkers. In this study, we demonstrate a workflow for surface protein discovery associated with cellular mechanophenotype. We sorted heterogeneous adipose-derived stem/stromal cells (ASCs) into groups with low vs. high lamin A/C, an intracellular protein linked to whole-cell mechanophenotype. Proteomic data of enriched groups identified surface protein candidates as potential biochemical proxies for ASC mechanophenotype. Select surface biomarkers were used for live-cell enrichment, with subsequent single-cell mechanical testing and lineage-specific differentiation. Ultimately, we identified CD44 to have a strong inverse correlation with whole-cell elastic modulus, with CD44lo cells exhibiting moduli three times greater than that of CD44hi cells. Functionally, these stiff and soft ASCs showed enhanced osteogenic and adipogenic differentiation potential, respectively. The described workflow can be replicated for any phenotype with a known correlated intracellular protein, allowing for the acquisition of live cells for further characterization, diagnostics, or therapeutics." @default.
- W4281853246 created "2022-06-13" @default.
- W4281853246 creator A5027046983 @default.
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- W4281853246 creator A5086933242 @default.
- W4281853246 date "2022-05-27" @default.
- W4281853246 modified "2023-10-14" @default.
- W4281853246 title "Discovery of surface biomarkers for cell mechanophenotype via an intracellular protein-based enrichment strategy" @default.
- W4281853246 cites W1517715606 @default.
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- W4281853246 cites W1965042694 @default.
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- W4281853246 cites W1974970489 @default.
- W4281853246 cites W1978405687 @default.
- W4281853246 cites W1979043529 @default.
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- W4281853246 cites W1991151250 @default.
- W4281853246 cites W1993824824 @default.
- W4281853246 cites W1994109845 @default.
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- W4281853246 cites W2022910278 @default.
- W4281853246 cites W2023649556 @default.
- W4281853246 cites W2026465178 @default.
- W4281853246 cites W2029782351 @default.
- W4281853246 cites W2030637965 @default.
- W4281853246 cites W2030642623 @default.
- W4281853246 cites W2037562745 @default.
- W4281853246 cites W2037632318 @default.
- W4281853246 cites W2037788386 @default.
- W4281853246 cites W2037999013 @default.
- W4281853246 cites W2042711576 @default.
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- W4281853246 cites W2125114181 @default.
- W4281853246 cites W2134208433 @default.
- W4281853246 cites W2149225679 @default.
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- W4281853246 cites W2167200020 @default.
- W4281853246 cites W2171887228 @default.
- W4281853246 cites W2272722996 @default.
- W4281853246 cites W2524341015 @default.
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- W4281853246 cites W2770037689 @default.
- W4281853246 cites W2774825525 @default.
- W4281853246 cites W2783874796 @default.
- W4281853246 cites W2793676899 @default.
- W4281853246 cites W2794340792 @default.
- W4281853246 cites W2797549363 @default.
- W4281853246 cites W2899503076 @default.
- W4281853246 cites W2908102529 @default.
- W4281853246 cites W2909369782 @default.
- W4281853246 cites W2964025210 @default.
- W4281853246 cites W2977678095 @default.
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- W4281853246 cites W3130304579 @default.
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- W4281853246 doi "https://doi.org/10.1007/s00018-022-04351-w" @default.
- W4281853246 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35622146" @default.
- W4281853246 hasPublicationYear "2022" @default.
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