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• W4281868082 abstract "Abstract Targeted protein degradation is an invaluable tool in studying the function of proteins. Such a tool was not available in Trypanosoma brucei , an evolutionarily divergent eukaryote that causes human African trypanosomiasis. Here, we have adapted degradFP (degrade Green Fluorescent Protein), a protein degradation system based on the SCF E3 ubiquitin ligase complex and anti-GFP nanobody, in T. brucei . As a proof of principle, we targeted a kinetoplastid kinetochore protein (KKT3) that constitutively localizes at kinetochores in the nucleus. Induction of degradFP in a cell line that had both alleles of KKT3 tagged with YFP caused more severe growth defect than RNAi in procyclic (insect form) cells. degradFP also worked on a cytoplasmic protein (COPII subunit, SEC31). Given the easiness in making GFP fusion cell lines in T. brucei , degradFP can serve as a powerful tool to rapidly deplete proteins of interest, especially those with low turnover rates." @default.
• W4281868082 created "2022-06-13" @default.
• W4281868082 creator A5003411797 @default.
• W4281868082 creator A5031038205 @default.
• W4281868082 date "2022-06-02" @default.
• W4281868082 modified "2023-09-27" @default.
• W4281868082 title "Targeted protein degradation using degradFP in <i>Trypanosoma brucei</i>" @default.
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• W4281868082 doi "https://doi.org/10.1101/2022.06.01.494430" @default.
• W4281868082 hasPublicationYear "2022" @default.
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