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• W4281899962 abstract "Abstract High value unsaturated fatty acids can be produced by de novo synthesis in microalgal cells, especially via heterotrophic cultivation. Unfortunately, the lipid accumulation of heterotrophic microalgae cannot be improved efficiently in conventional ways. Here we reported heterotrophic Tribonema minus , a promising resource for the production of palmitoleic acid which has increasing demands in health service for patients with metabolic syndrome, as whole‐cell biocatalyst to develop a novel way of shifting low value exogenous saturated fatty acids to high value ones. Results showed that myristic acid is the best precursor for whole‐cell catalysis; it elevated the lipid content of T. minus to 42.2%, the highest among the tried precursors. The influences of cultivation condition on the utilization of extrinsic myristic acid and lipid accumulation were also determined. Under the optimized condition, the lipid content reached as high as 48.9%. In addition, our findings showed that ~13.0% of C16:1 in T. minus is derived from extrinsic myristic acid, and 30.1% of metabolized precursor is converted into heterologous fatty acids. Thus, a feasible approach for both increasing the value of low value saturated fatty acid by bioconversion and enhancing the lipid accumulation in microalgae is proposed by supplementing extrinsic myristic acid." @default.
• W4281899962 created "2022-06-13" @default.
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• W4281899962 date "2022-06-21" @default.
• W4281899962 modified "2023-09-27" @default.
• W4281899962 title "Producing high value unsaturated fatty acid by whole‐cell catalysis using microalga: A case study with <i>Tribonema minus</i>" @default.
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• W4281899962 doi "https://doi.org/10.1002/bit.28157" @default.
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