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• W4281901209 endingPage "2000" @default.
• W4281901209 startingPage "1992" @default.
• W4281901209 abstract "Rhamnolipids (RLs) are biosurfactants with great economic significance that have been used extensively in multiple industries. Pseudomonas aeruginosa is a promising microorganism for sustainable RL production. However, current CTAB-MB based screening of RL-producing strains is time-consuming, labor-intensive, and unable to distinguish mono- and di-RL. In this study, we developed a novel transcription-associated fluorescence-activated droplet sorting (FADS) method to specifically target the di-RL hyperproducers. We first investigated critical factors associated with this method, including the specificity and sensitivity for discriminating di-RL overproducers from other communities. Validation of genotype-phenotype linkage between the GFP intensity, rhlC transcription, and di-RL production showed that rhlC transcription is closely correlated with di-RL production, and the GFP intensity is responsive to rhlC transcription, respectively. Using this platform, we screened out ten higher di-RL producing microorganisms, which produced 54-208% more di-RL than the model P. aeruginosa PAO1. In summary, the droplet-based microfluidic platform not only facilitates a more specific, reliable, and rapid screening of P. aeruginosa colonies with desired phenotypes, but also shows that intracellular transcription-associated GFP intensity can be used to measure the yield of di-RL between populations of droplets containing different environmental colonies. This method also can be integrated with transposon mutation libraries to target P. aeruginosa mutants." @default.
• W4281901209 created "2022-06-13" @default.
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• W4281901209 date "2022-05-31" @default.
• W4281901209 modified "2023-10-18" @default.
• W4281901209 title "Transcription-Associated Fluorescence-Activated Droplet Sorting for Di-rhamnolipid Hyperproducers" @default.
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• W4281901209 doi "https://doi.org/10.1021/acssynbio.1c00622" @default.
• W4281901209 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35640073" @default.
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