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- W4281956851 endingPage "19963" @default.
- W4281956851 startingPage "19957" @default.
- W4281956851 abstract "Loop-mediated isothermal amplification (LAMP) has been widely used in nucleic acid assay because of its high specificity, sensitivity, and isothermal property. However, the complexity of amplification product detection is still a major challenge for its wide applications. Herein, we developed a light scattering technology-assisted, low-cost, and simple detection manner of LAMP products without expensive reagents and complicated instruments. Only needing to add a kind of strong acid to the amplification products, the amplification products can aggregate into large particles in a strongly acidic medium, and large particles can produce strong light scattering, which shows a good proportional relationship with the number of amplification products in a wide range. The proposed method shows excellent sensitivity and high specificity that can quantify RNA as low as 100 aM with a single-base resolution." @default.
- W4281956851 created "2022-06-13" @default.
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- W4281956851 date "2022-06-01" @default.
- W4281956851 modified "2023-10-17" @default.
- W4281956851 title "Light Scattering Technology-Combined Ligation-Dependent Loop-Mediated Isothermal Amplification (LL-LAMP) for Sensitive Detection of RNA" @default.
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- W4281956851 doi "https://doi.org/10.1021/acsomega.2c01759" @default.
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