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• W4281978051 endingPage "R330" @default.
• W4281978051 startingPage "R319" @default.
• W4281978051 abstract "The peroxisome proliferator-activated receptor γ coactivator 1 α (PGC-1α) is central to the regulation of cellular and mitochondrial energy homeostasis in mammals, but its role in other vertebrates remains unclear. Indeed, previous work suggests extensive structural and functional divergence of PGC-1α in teleosts but this remains to be directly tested. Here, we describe the initial characterization of heterozygous PGC-1α mutant zebrafish lines created by CRISPR-Cas9 disruptions of an evolutionarily conserved regulatory region of the PGC-1α proximal promoter. Using qPCR, we confirmed the disruption of PGC-1α gene expression in striated muscle, leading to a simultaneous fourfold increase in mixed skeletal muscle PGC-1α mRNA levels and an opposite fourfold downregulation in cardiac muscle. In mixed skeletal muscle, most downstream effector genes were largely unaffected yet two mitochondrial lipid transporters, carnitine palmitoyltransferase-1 and -2, were strongly induced. Conversely, PGC-1α depression in cardiac muscle reduced the expression of several transcriptional regulators (estrogen-related receptor α, nuclear respiratory factor 1, and PGC-1β) without altering metabolic gene expression. Using high-resolution respirometry, we determined that white muscle exhibited increased lipid oxidative capacity with little difference in markers of mitochondrial abundance. Finally, using whole animal intermittent respirometry, we show that mutant fish exhibit a twofold higher basal metabolism than their wild-type counterparts. Altogether, this new model confirms a central but complex role for PGC-1α in mediating energy utilization in zebrafish, and we propose its use as a valuable tool to explore the intricate regulatory pathways of energy homeostasis in a popular biomedical model." @default.
• W4281978051 created "2022-06-13" @default.
• W4281978051 creator A5017810486 @default.
• W4281978051 creator A5053877869 @default.
• W4281978051 creator A5077278637 @default.
• W4281978051 date "2022-09-01" @default.
• W4281978051 modified "2023-09-27" @default.
• W4281978051 title "Metabolic consequences of PGC-1α dysregulation in adult zebrafish muscle" @default.
• W4281978051 cites W1496238527 @default.
• W4281978051 cites W1505090413 @default.
• W4281978051 cites W1577872659 @default.
• W4281978051 cites W1585179940 @default.
• W4281978051 cites W1626664198 @default.
• W4281978051 cites W1665938856 @default.
• W4281978051 cites W1857766814 @default.
• W4281978051 cites W1971082641 @default.
• W4281978051 cites W1980542694 @default.
• W4281978051 cites W1994544848 @default.
• W4281978051 cites W1996821192 @default.
• W4281978051 cites W2000065704 @default.
• W4281978051 cites W2004232360 @default.
• W4281978051 cites W2009727112 @default.
• W4281978051 cites W2014276824 @default.
• W4281978051 cites W2014300581 @default.
• W4281978051 cites W2017011351 @default.
• W4281978051 cites W2028339345 @default.
• W4281978051 cites W2034767282 @default.
• W4281978051 cites W2035705986 @default.
• W4281978051 cites W2048771411 @default.
• W4281978051 cites W2053765200 @default.
• W4281978051 cites W2071631797 @default.
• W4281978051 cites W2078220726 @default.
• W4281978051 cites W2087389024 @default.
• W4281978051 cites W2089621556 @default.
• W4281978051 cites W2097315431 @default.
• W4281978051 cites W2098412979 @default.
• W4281978051 cites W2102743559 @default.
• W4281978051 cites W2105404724 @default.
• W4281978051 cites W2107348193 @default.
• W4281978051 cites W2109635567 @default.
• W4281978051 cites W2110374590 @default.
• W4281978051 cites W2120162471 @default.
• W4281978051 cites W2120293566 @default.
• W4281978051 cites W2123490534 @default.
• W4281978051 cites W2126337135 @default.
• W4281978051 cites W2128211963 @default.
• W4281978051 cites W2131117779 @default.
• W4281978051 cites W2134567873 @default.
• W4281978051 cites W2136985950 @default.
• W4281978051 cites W2140751228 @default.
• W4281978051 cites W2145944506 @default.
• W4281978051 cites W2148831368 @default.
• W4281978051 cites W2156066001 @default.
• W4281978051 cites W2157334943 @default.
• W4281978051 cites W2161570633 @default.
• W4281978051 cites W2162098634 @default.
• W4281978051 cites W2166810075 @default.
• W4281978051 cites W2168948848 @default.
• W4281978051 cites W2169187666 @default.
• W4281978051 cites W2174064905 @default.
• W4281978051 cites W2174243247 @default.
• W4281978051 cites W2185288834 @default.
• W4281978051 cites W2186315818 @default.
• W4281978051 cites W2215460973 @default.
• W4281978051 cites W2224350866 @default.
• W4281978051 cites W2237137237 @default.
• W4281978051 cites W2314146467 @default.
• W4281978051 cites W2322353344 @default.
• W4281978051 cites W2336470207 @default.
• W4281978051 cites W2345388247 @default.
• W4281978051 cites W2441333409 @default.
• W4281978051 cites W2494669249 @default.
• W4281978051 cites W2726817246 @default.
• W4281978051 cites W2777003387 @default.
• W4281978051 cites W2778329740 @default.
• W4281978051 cites W2780698132 @default.
• W4281978051 cites W2793903410 @default.
• W4281978051 cites W2805752574 @default.
• W4281978051 cites W2808345397 @default.
• W4281978051 cites W2810285385 @default.
• W4281978051 cites W2883880202 @default.
• W4281978051 cites W2885238014 @default.
• W4281978051 cites W2917091236 @default.
• W4281978051 cites W2937017485 @default.
• W4281978051 cites W2942817795 @default.
• W4281978051 cites W2946563505 @default.
• W4281978051 cites W2949475756 @default.
• W4281978051 cites W2955799898 @default.
• W4281978051 cites W2963836170 @default.
• W4281978051 cites W2975732349 @default.
• W4281978051 cites W2979989133 @default.
• W4281978051 cites W3007758236 @default.
• W4281978051 cites W3138401678 @default.
• W4281978051 cites W77467296 @default.
• W4281978051 cites W917998901 @default.
• W4281978051 doi "https://doi.org/10.1152/ajpregu.00188.2021" @default.
• W4281978051 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35670765" @default.
• W4281978051 hasPublicationYear "2022" @default.

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