Matches in SemOpenAlex for { <https://semopenalex.org/work/W4281982802> ?p ?o ?g. }
- W4281982802 abstract "The osteogenic potential of bone marrow mesenchymal stem cells (BMSCs) is critical for bone formation and regeneration. A high non-/delayed-union rate of fracture healing still occurs in specific populations, implying an urgent need to discover novel targets for promoting osteogenesis and bone regeneration. Long non-coding (lnc)RNAs are emerging regulators of multiple physiological processes, including osteogenesis. Based on differential expression analysis of RNA sequencing data, we found that lncRNA AC132217.4, a 3'UTR-overlapping lncRNA of insulin growth factor 2 (IGF2), was highly induced during osteogenic differentiation of BMSCs. Afterward, both gain-of-function and loss-of-function experiments proved that AC132217.4 promotes osteoblast development from BMSCs. As for its molecular mechanism, we found that AC132217.4 binds with IGF2 mRNA to regulate its expression and downstream AKT activation to control osteoblast maturation and function. Furthermore, we identified two splicing factors, splicing component 35 KDa (SC35) and heterogeneous nuclear ribonucleoprotein A1 (HNRNPA1), which regulate the biogenesis of AC132217.4 at the post-transcriptional level. We also identified a transcription factor, ALX1, which regulates AC132217.7 expression at the transcriptional level to promote osteogenesis. Importantly, in-vivo over-expression of AC132217.4 essentially promotes the bone healing process in a murine tibial drill-hole model. Our study demonstrates that lncRNA AC132217.4 is a novel anabolic regulator of BMSC osteogenesis and could be a plausible therapeutic target for improving bone regeneration." @default.
- W4281982802 created "2022-06-13" @default.
- W4281982802 creator A5003293441 @default.
- W4281982802 creator A5003565023 @default.
- W4281982802 creator A5017058186 @default.
- W4281982802 creator A5024007738 @default.
- W4281982802 creator A5043370802 @default.
- W4281982802 creator A5051258987 @default.
- W4281982802 date "2022-05-31" @default.
- W4281982802 modified "2023-10-15" @default.
- W4281982802 title "ALX1-transcribed LncRNA AC132217.4 promotes osteogenesis and bone healing via IGF-AKT signaling in mesenchymal stem cells" @default.
- W4281982802 cites W1048885802 @default.
- W4281982802 cites W1513928844 @default.
- W4281982802 cites W1531899892 @default.
- W4281982802 cites W1854383303 @default.
- W4281982802 cites W1972206562 @default.
- W4281982802 cites W1980231989 @default.
- W4281982802 cites W1981564090 @default.
- W4281982802 cites W1991972761 @default.
- W4281982802 cites W1993982785 @default.
- W4281982802 cites W1996361385 @default.
- W4281982802 cites W1998983041 @default.
- W4281982802 cites W2003962767 @default.
- W4281982802 cites W2008052808 @default.
- W4281982802 cites W2010609060 @default.
- W4281982802 cites W2014104304 @default.
- W4281982802 cites W2026639349 @default.
- W4281982802 cites W2032183472 @default.
- W4281982802 cites W2033807339 @default.
- W4281982802 cites W2038682678 @default.
- W4281982802 cites W2046008810 @default.
- W4281982802 cites W2059437739 @default.
- W4281982802 cites W2082750644 @default.
- W4281982802 cites W2084990113 @default.
- W4281982802 cites W2088003694 @default.
- W4281982802 cites W2089549631 @default.
- W4281982802 cites W2113895515 @default.
- W4281982802 cites W2114541039 @default.
- W4281982802 cites W2119287599 @default.
- W4281982802 cites W2119610794 @default.
- W4281982802 cites W2128603683 @default.
- W4281982802 cites W2132726972 @default.
- W4281982802 cites W2138305701 @default.
- W4281982802 cites W2141337702 @default.
- W4281982802 cites W2144337206 @default.
- W4281982802 cites W2148067910 @default.
- W4281982802 cites W2153268494 @default.
- W4281982802 cites W2168141899 @default.
- W4281982802 cites W2183568263 @default.
- W4281982802 cites W2198153867 @default.
- W4281982802 cites W2319901133 @default.
- W4281982802 cites W2331330656 @default.
- W4281982802 cites W2343938601 @default.
- W4281982802 cites W2613662456 @default.
- W4281982802 cites W2623277425 @default.
- W4281982802 cites W2748110529 @default.
- W4281982802 cites W2750746460 @default.
- W4281982802 cites W2752372333 @default.
- W4281982802 cites W2770504522 @default.
- W4281982802 cites W2775040869 @default.
- W4281982802 cites W2790014095 @default.
- W4281982802 cites W2792055257 @default.
- W4281982802 cites W2795601394 @default.
- W4281982802 cites W2808973838 @default.
- W4281982802 cites W2900865173 @default.
- W4281982802 cites W2904813623 @default.
- W4281982802 cites W2908413325 @default.
- W4281982802 cites W2928609784 @default.
- W4281982802 cites W2971552833 @default.
- W4281982802 cites W2982509833 @default.
- W4281982802 cites W2997574469 @default.
- W4281982802 cites W3011603952 @default.
- W4281982802 cites W3084574727 @default.
- W4281982802 cites W3085150136 @default.
- W4281982802 cites W3088511309 @default.
- W4281982802 cites W3115880421 @default.
- W4281982802 cites W3154628694 @default.
- W4281982802 cites W3159024074 @default.
- W4281982802 cites W3159439354 @default.
- W4281982802 cites W4205899298 @default.
- W4281982802 cites W4210738618 @default.
- W4281982802 cites W945503290 @default.
- W4281982802 doi "https://doi.org/10.1007/s00018-022-04338-7" @default.
- W4281982802 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35639207" @default.
- W4281982802 hasPublicationYear "2022" @default.
- W4281982802 type Work @default.
- W4281982802 citedByCount "4" @default.
- W4281982802 countsByYear W42819828022022 @default.
- W4281982802 countsByYear W42819828022023 @default.
- W4281982802 crossrefType "journal-article" @default.
- W4281982802 hasAuthorship W4281982802A5003293441 @default.
- W4281982802 hasAuthorship W4281982802A5003565023 @default.
- W4281982802 hasAuthorship W4281982802A5017058186 @default.
- W4281982802 hasAuthorship W4281982802A5024007738 @default.
- W4281982802 hasAuthorship W4281982802A5043370802 @default.
- W4281982802 hasAuthorship W4281982802A5051258987 @default.
- W4281982802 hasBestOaLocation W42819828022 @default.
- W4281982802 hasConcept C104317684 @default.
- W4281982802 hasConcept C185592680 @default.
- W4281982802 hasConcept C198826908 @default.