Matches in SemOpenAlex for { <https://semopenalex.org/work/W4282000534> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W4282000534 abstract "The Aedes aegypti mosquito is a major vector of chikungunya virus (CHIKV), which has no licensed vaccine. Engineered mosquitoes expressing long RNA hairpins (lhRNA) or small RNAs against selected arboviruses have been developed to limit virus replication, but their silencing efficiency has not been compared. We developed lhRNA and short hairpin RNA (shRNA) arrays against CHIKV non-structural protein nsP2. We used a Tet-response element (TRE) and a tTA transactivator to control expression of lhRNAs (TRE-lhRNA) and shRNAs (TRE-shRNA). Constitutive expression in Aag2 cells was assessed with a PUb-tTA driverand midgut specific expression in transgenic mosquitoes was assessed using Carboxypeptidase A (AeCPA)-tTA as driver. In vitrointerference ability was determined with a CHIKV split replication system, and a synthetic luciferase reporter with mRNA containing the targeted CHIKV sequence (CHIK-FF). In vivo interference was tested by inserting the TRE-lhRNA and TRE-shRNA constructs into a AeCPA-tTA line so both constructs expressed from the same locus, and a TRE reporter line that expressed an AmCyan reporter with an N terminal CHIKV fusion (CHIK-AmC). In Aag2 cells, shRNAs were more effective than lhRNA in silencing both the CHIKV split replication system (99.7%, S. D. ± 0.47 and 72.8%, S. D. ± 9.50, respectively, P<0.001) and CHIK-FF (98.8%, S. D. ± 0.71 and 50.9%, S. D. ± 7.92, respectively, P<0.05). Similar results were observed in transgenic mosquitoes when comparing AmC expression in the midgut. This study demonstrates that, in mosquitoes, effectively chosen shRNAs can induce greater interference of the desired viral target than the corresponding lhRNA." @default.
- W4282000534 created "2022-06-13" @default.
- W4282000534 creator A5015549381 @default.
- W4282000534 creator A5021118815 @default.
- W4282000534 creator A5035829003 @default.
- W4282000534 creator A5048409245 @default.
- W4282000534 creator A5057697948 @default.
- W4282000534 creator A5058085689 @default.
- W4282000534 creator A5080548435 @default.
- W4282000534 creator A5087999743 @default.
- W4282000534 creator A5088503493 @default.
- W4282000534 date "2022-05-27" @default.
- W4282000534 modified "2023-09-29" @default.
- W4282000534 title "Engineered anti-viral shRNAs are more effective than lhRNAs in transgenic Aedes aegypti" @default.
- W4282000534 doi "https://doi.org/10.1099/acmi.ac2021.po0421" @default.
- W4282000534 hasPublicationYear "2022" @default.
- W4282000534 type Work @default.
- W4282000534 citedByCount "0" @default.
- W4282000534 crossrefType "journal-article" @default.
- W4282000534 hasAuthorship W4282000534A5015549381 @default.
- W4282000534 hasAuthorship W4282000534A5021118815 @default.
- W4282000534 hasAuthorship W4282000534A5035829003 @default.
- W4282000534 hasAuthorship W4282000534A5048409245 @default.
- W4282000534 hasAuthorship W4282000534A5057697948 @default.
- W4282000534 hasAuthorship W4282000534A5058085689 @default.
- W4282000534 hasAuthorship W4282000534A5080548435 @default.
- W4282000534 hasAuthorship W4282000534A5087999743 @default.
- W4282000534 hasAuthorship W4282000534A5088503493 @default.
- W4282000534 hasBestOaLocation W42820005341 @default.
- W4282000534 hasConcept C104317684 @default.
- W4282000534 hasConcept C119056186 @default.
- W4282000534 hasConcept C140704245 @default.
- W4282000534 hasConcept C153911025 @default.
- W4282000534 hasConcept C159047783 @default.
- W4282000534 hasConcept C166703698 @default.
- W4282000534 hasConcept C173396325 @default.
- W4282000534 hasConcept C173758957 @default.
- W4282000534 hasConcept C190232843 @default.
- W4282000534 hasConcept C22615655 @default.
- W4282000534 hasConcept C2522874641 @default.
- W4282000534 hasConcept C2777775583 @default.
- W4282000534 hasConcept C2781273456 @default.
- W4282000534 hasConcept C40767141 @default.
- W4282000534 hasConcept C54009773 @default.
- W4282000534 hasConcept C54355233 @default.
- W4282000534 hasConcept C59822182 @default.
- W4282000534 hasConcept C67705224 @default.
- W4282000534 hasConcept C81885089 @default.
- W4282000534 hasConcept C86803240 @default.
- W4282000534 hasConcept C92087593 @default.
- W4282000534 hasConceptScore W4282000534C104317684 @default.
- W4282000534 hasConceptScore W4282000534C119056186 @default.
- W4282000534 hasConceptScore W4282000534C140704245 @default.
- W4282000534 hasConceptScore W4282000534C153911025 @default.
- W4282000534 hasConceptScore W4282000534C159047783 @default.
- W4282000534 hasConceptScore W4282000534C166703698 @default.
- W4282000534 hasConceptScore W4282000534C173396325 @default.
- W4282000534 hasConceptScore W4282000534C173758957 @default.
- W4282000534 hasConceptScore W4282000534C190232843 @default.
- W4282000534 hasConceptScore W4282000534C22615655 @default.
- W4282000534 hasConceptScore W4282000534C2522874641 @default.
- W4282000534 hasConceptScore W4282000534C2777775583 @default.
- W4282000534 hasConceptScore W4282000534C2781273456 @default.
- W4282000534 hasConceptScore W4282000534C40767141 @default.
- W4282000534 hasConceptScore W4282000534C54009773 @default.
- W4282000534 hasConceptScore W4282000534C54355233 @default.
- W4282000534 hasConceptScore W4282000534C59822182 @default.
- W4282000534 hasConceptScore W4282000534C67705224 @default.
- W4282000534 hasConceptScore W4282000534C81885089 @default.
- W4282000534 hasConceptScore W4282000534C86803240 @default.
- W4282000534 hasConceptScore W4282000534C92087593 @default.
- W4282000534 hasIssue "5" @default.
- W4282000534 hasLocation W42820005341 @default.
- W4282000534 hasOpenAccess W4282000534 @default.
- W4282000534 hasPrimaryLocation W42820005341 @default.
- W4282000534 hasRelatedWork W1974689980 @default.
- W4282000534 hasRelatedWork W2023001964 @default.
- W4282000534 hasRelatedWork W2042993634 @default.
- W4282000534 hasRelatedWork W2047283130 @default.
- W4282000534 hasRelatedWork W2088835981 @default.
- W4282000534 hasRelatedWork W2164551093 @default.
- W4282000534 hasRelatedWork W2373539659 @default.
- W4282000534 hasRelatedWork W2387605231 @default.
- W4282000534 hasRelatedWork W2414655079 @default.
- W4282000534 hasRelatedWork W3096142661 @default.
- W4282000534 hasVolume "4" @default.
- W4282000534 isParatext "false" @default.
- W4282000534 isRetracted "false" @default.
- W4282000534 workType "article" @default.