Matches in SemOpenAlex for { <https://semopenalex.org/work/W4282025462> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W4282025462 abstract "Many genes encoding nucleotide-binding leucine-rich repeat receptors (NLRs) are regulated and fine-tuned by miR482 to balance the trade-off between disease resistance and growth. Dicotyledonous plants, including cotton, usually have multiple miR482 isoforms. Each miR482 isoform can regulate several NLRs that in turn can be regulated by several different miR482 isoforms. Dissecting the functionality of individual miR482 isoforms in disease response and in balancing the disease resistance and growth trade-off demands a collection of mutants mutated in individual miR482 members (single or multiple). In this study, we generated such a collection of cotton miR482 mutants using CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats/Cas9) genome editing and transformation of pooled guide RNAs (gRNAs). In total, 84 T0 plants representing 40 independent transgenic events and harboring mutation in each of the 10 miR482 isoforms were generated. The average editing efficiency of the 18 transformed gRNAs is 75%, ranging from 0 (3 gRNAs) to 100% (8 gRNAs). Most miR482 isoforms have a diverse range of mutations, including small indels (1-44 bp) and substitutions, which are expected to impair biogenesis of miR482. All nine mutant populations used in Verticillium dahliae infection experiments showed a disease index lower than the control, with four being significantly lower. The disease assay also suggests a different role of different miR482 isoforms in disease response and a potential dosage effect of miR482l. The study demonstrates the feasibility of saturation mutagenesis of plant miRNA families with dozens of genetic loci using CRISPR/Cas9 and provides the cotton community a valuable resource for uncovering the miR482-NLR module(s) underlying the interaction between cotton and different pathogens." @default.
- W4282025462 created "2022-06-13" @default.
- W4282025462 creator A5017941291 @default.
- W4282025462 creator A5026580535 @default.
- W4282025462 creator A5057814872 @default.
- W4282025462 creator A5059480616 @default.
- W4282025462 creator A5073847483 @default.
- W4282025462 creator A5084893043 @default.
- W4282025462 date "2022-06-01" @default.
- W4282025462 modified "2023-10-16" @default.
- W4282025462 title "CRISPR/Cas9‐mediated saturated mutagenesis of the cotton <i>MIR482</i> family for dissecting the functionality of individual members in disease response" @default.
- W4282025462 cites W1979604069 @default.
- W4282025462 cites W1990379499 @default.
- W4282025462 cites W2001845502 @default.
- W4282025462 cites W2015337585 @default.
- W4282025462 cites W2021767455 @default.
- W4282025462 cites W2093776734 @default.
- W4282025462 cites W209968627 @default.
- W4282025462 cites W2138067720 @default.
- W4282025462 cites W252444366 @default.
- W4282025462 cites W2612818194 @default.
- W4282025462 cites W2657928257 @default.
- W4282025462 cites W2665695513 @default.
- W4282025462 cites W2673510916 @default.
- W4282025462 cites W2792145175 @default.
- W4282025462 cites W2886593508 @default.
- W4282025462 cites W2902012935 @default.
- W4282025462 cites W2939081343 @default.
- W4282025462 cites W2960786686 @default.
- W4282025462 cites W2970119199 @default.
- W4282025462 cites W3007037748 @default.
- W4282025462 cites W3037845557 @default.
- W4282025462 cites W3038199869 @default.
- W4282025462 cites W3080158858 @default.
- W4282025462 cites W3088923596 @default.
- W4282025462 cites W3108948459 @default.
- W4282025462 cites W3116914038 @default.
- W4282025462 cites W3128770082 @default.
- W4282025462 cites W3129169955 @default.
- W4282025462 cites W3214681872 @default.
- W4282025462 cites W4210962045 @default.
- W4282025462 cites W4211020888 @default.
- W4282025462 cites W4282025462 @default.
- W4282025462 doi "https://doi.org/10.1002/pld3.410" @default.
- W4282025462 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35685042" @default.
- W4282025462 hasPublicationYear "2022" @default.
- W4282025462 type Work @default.
- W4282025462 citedByCount "8" @default.
- W4282025462 countsByYear W42820254622022 @default.
- W4282025462 countsByYear W42820254622023 @default.
- W4282025462 crossrefType "journal-article" @default.
- W4282025462 hasAuthorship W4282025462A5017941291 @default.
- W4282025462 hasAuthorship W4282025462A5026580535 @default.
- W4282025462 hasAuthorship W4282025462A5057814872 @default.
- W4282025462 hasAuthorship W4282025462A5059480616 @default.
- W4282025462 hasAuthorship W4282025462A5073847483 @default.
- W4282025462 hasAuthorship W4282025462A5084893043 @default.
- W4282025462 hasBestOaLocation W42820254621 @default.
- W4282025462 hasConcept C104317684 @default.
- W4282025462 hasConcept C132455925 @default.
- W4282025462 hasConcept C143065580 @default.
- W4282025462 hasConcept C144501496 @default.
- W4282025462 hasConcept C16318435 @default.
- W4282025462 hasConcept C53345823 @default.
- W4282025462 hasConcept C54355233 @default.
- W4282025462 hasConcept C70721500 @default.
- W4282025462 hasConcept C86803240 @default.
- W4282025462 hasConcept C98108389 @default.
- W4282025462 hasConceptScore W4282025462C104317684 @default.
- W4282025462 hasConceptScore W4282025462C132455925 @default.
- W4282025462 hasConceptScore W4282025462C143065580 @default.
- W4282025462 hasConceptScore W4282025462C144501496 @default.
- W4282025462 hasConceptScore W4282025462C16318435 @default.
- W4282025462 hasConceptScore W4282025462C53345823 @default.
- W4282025462 hasConceptScore W4282025462C54355233 @default.
- W4282025462 hasConceptScore W4282025462C70721500 @default.
- W4282025462 hasConceptScore W4282025462C86803240 @default.
- W4282025462 hasConceptScore W4282025462C98108389 @default.
- W4282025462 hasFunder F4320330857 @default.
- W4282025462 hasIssue "6" @default.
- W4282025462 hasLocation W42820254621 @default.
- W4282025462 hasLocation W42820254622 @default.
- W4282025462 hasLocation W42820254623 @default.
- W4282025462 hasOpenAccess W4282025462 @default.
- W4282025462 hasPrimaryLocation W42820254621 @default.
- W4282025462 hasRelatedWork W2134268326 @default.
- W4282025462 hasRelatedWork W2527347512 @default.
- W4282025462 hasRelatedWork W2748004056 @default.
- W4282025462 hasRelatedWork W2770152187 @default.
- W4282025462 hasRelatedWork W2900771990 @default.
- W4282025462 hasRelatedWork W2943124855 @default.
- W4282025462 hasRelatedWork W2949273383 @default.
- W4282025462 hasRelatedWork W2953290252 @default.
- W4282025462 hasRelatedWork W3185119339 @default.
- W4282025462 hasRelatedWork W4205349507 @default.
- W4282025462 hasVolume "6" @default.
- W4282025462 isParatext "false" @default.
- W4282025462 isRetracted "false" @default.
- W4282025462 workType "article" @default.