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- W4282044489 abstract "Abstract Difficulty achieving complete, specific, and homogenous staining is a major bottleneck preventing the widespread use of tissue clearing techniques to image large volumes of human tissue. In this manuscript, we describe a procedure to rapidly design immunostaining protocols for antibody labeling of cleared brain tissue. We prepared libraries of .5-1.0 mm thick tissue sections that are fixed, pre-treated, and cleared via similar, but different procedures to optimize staining conditions for a panel of antibodies. Results from a library of mouse tissue correlate well with results from a similarly prepared library of human brain tissue, suggesting mouse tissue is an adequate substitute for protocol optimization. These data show that procedural differences do not influence every antibody-antigen pair in the same way, and minor changes can have deleterious effects, therefore, optimization should be conducted for each target. The approach outlined here will help guide researchers to successfully label a variety of targets, thus removing a major hurdle to accessing the rich 3D information available in large, cleared human tissue volumes." @default.
- W4282044489 created "2022-06-13" @default.
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- W4282044489 date "2022-05-26" @default.
- W4282044489 modified "2023-09-25" @default.
- W4282044489 title "Tissue libraries enable rapid determination of conditions that preserve antibody labeling in cleared mouse and human tissue" @default.
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- W4282044489 doi "https://doi.org/10.1101/2022.05.25.493465" @default.
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