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- W4282549265 abstract "Abstract Pseudomonas aeruginosa ( P. aeruginosa ) is a major pathogen that causes nosocomial infections and often exhibits antibiotic resistance. Therefore, the development of an accurate method for detecting P. aeruginosa is required to control P. aeruginosa -related outbreaks. In this study, we established an enzyme-linked immunosorbent assay (ELISA) method for the sensitive detection of three P. aeruginosa strains, UCBPP PA14, ATCC 27853, and multidrug-resistant ATCC BAA-2108. We produced a recombinant antibody (rAb) against P. aeruginosa using mammalian cells with high yield and purity, and confirmed its P. aeruginosa binding efficiency. The rAb was paired with commercial anti- P. aeruginosa Ab for a sandwich ELISA, resulting in an antigen-concentration- dependent response with a limit of detection value of 23 CFU. These results suggest that the rAb produced herein can be used for the sensitive detection of P. aeruginosa with a wide range of applications in clinical diagnosis and point-of-care testing." @default.
- W4282549265 created "2022-06-14" @default.
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- W4282549265 date "2022-06-13" @default.
- W4282549265 modified "2023-09-23" @default.
- W4282549265 title "Generation of a novel recombinant antibody for sensitive detection of Pseudomonas aeruginosa" @default.
- W4282549265 doi "https://doi.org/10.21203/rs.3.rs-1717794/v1" @default.
- W4282549265 hasPublicationYear "2022" @default.
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