FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4282559105> ?p ?o ?g. }

• W4282559105 endingPage "110403" @default.
• W4282559105 startingPage "110403" @default.
• W4282559105 abstract "Keloid is a benign proliferative disease characterized by excessive deposition of extracellular matrix collagen during skin wound healing. The mechanisms of keloid formation have not been fully elucidated, and the current treatment methods are not effective for all keloid patients. Therefore, there is an urgent need to find more effective therapies, and our research focused on identifying characteristic molecular signatures of keloid to explore potential therapeutic targets.Gene expression profiles of keloid and control group samples were retrieved from the GEO database. Taking the GSE113619 dataset as the training set, the dataset collected skin tissues from non-lesion sites of healthy and keloid-prone individuals, denoted as Day0. The second sampling was performed 42 days later at the original sampling site of control and keloid groups, denoted as Day42.The 'limma' package and Venn diagram identified differentially expressed genes (DEGs) specific to keloid day42 versus day0 samples. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), Reactome pathway functional enrichment, and annotation of the characteristic genes were conducted on the Metascape website. Ingenuity canonical pathways, disease & function enrichment analysis and gene interaction network were performed and predicted in Ingenuity Pathway Analysis (IPA) software. Key module genes related to keloid were filtered out by Weighted Gene Co-expression Network Analysis (WGCNA). We utilized the Least Absolute Shrinkage and Selection Operator (LASSO) algorithm to screen the characteristic genes in keloid by the 'glmnet' package. The area under the curve (AUC) of receiver operating characteristic (ROC) was utilized to determine the effectiveness of potential signatures in discriminating keloid samples from normal samples and performed by using the 'pROC' package. The enrich scores of 24 immune cells in each sample were calculated by the single-sample gene set enrichment analysis (ssGSEA) algorithm， and then the Gene Set Variation Analysis (GSVA) was performed. Finally, RNA from 4 normal and 6 keloid samples was extracted, and RT-qPCR and Western Blot validated the expression of characteristic genes.A total of 640 DEGs specific to keloid day42 versus day0 samples were detected. 69 key module genes were uncovered and implicated in 'NCAM signaling for neurite out-growth', 'oncogenic MAPK signaling', 'transmission across chemical synapses' pathways, and the mitotic cell cycle-related processes. Five characteristic genes (MTUS1, UNC5C, CEP57, NAA35, and HOXD3) of keloid were identified by LASSO, and among which UNC5C and HOXD3 were validated by ROC plot in external dataset, RT-qPCR and Western Blot in validation samples. The result of ssGSEA indicated that the infiltration of neutrophils showed a relatively higher abundance and natural killer cells with relatively low enrichment in the keloid group compared to the control group. UNC5C was correlated with more immune cells compared with other characteristic genes.In this study, characteristic genes associated with keloid were identified by bioinformatic approaches and verified in clinical validation samples, providing potential targets for the diagnosis and treatment of keloid." @default.
• W4282559105 created "2022-06-14" @default.
• W4282559105 creator A5002322160 @default.
• W4282559105 creator A5010738468 @default.
• W4282559105 creator A5012993508 @default.
• W4282559105 creator A5019985710 @default.
• W4282559105 creator A5027861145 @default.
• W4282559105 creator A5048542794 @default.
• W4282559105 creator A5049078993 @default.
• W4282559105 creator A5081456977 @default.
• W4282559105 date "2022-07-01" @default.
• W4282559105 modified "2023-10-14" @default.
• W4282559105 title "Identification and validation of HOXD3 and UNC5C as molecular signatures in keloid based on weighted gene co-expression network analysis" @default.
• W4282559105 cites W1963516632 @default.
• W4282559105 cites W1966107523 @default.
• W4282559105 cites W1966327575 @default.
• W4282559105 cites W1972559856 @default.
• W4282559105 cites W1994276113 @default.
• W4282559105 cites W1995650710 @default.
• W4282559105 cites W2000865288 @default.
• W4282559105 cites W2006617902 @default.
• W4282559105 cites W2014116770 @default.
• W4282559105 cites W2016477139 @default.
• W4282559105 cites W2031702481 @default.
• W4282559105 cites W2036104995 @default.
• W4282559105 cites W2041056430 @default.
• W4282559105 cites W2042720684 @default.
• W4282559105 cites W2054214343 @default.
• W4282559105 cites W2067786443 @default.
• W4282559105 cites W2075061052 @default.
• W4282559105 cites W2105558032 @default.
• W4282559105 cites W2107277218 @default.
• W4282559105 cites W2120558322 @default.
• W4282559105 cites W2146512944 @default.
• W4282559105 cites W2159707944 @default.
• W4282559105 cites W2162143298 @default.
• W4282559105 cites W2163278196 @default.
• W4282559105 cites W2345009038 @default.
• W4282559105 cites W2382964851 @default.
• W4282559105 cites W2577263397 @default.
• W4282559105 cites W2593918601 @default.
• W4282559105 cites W2779053392 @default.
• W4282559105 cites W2806429275 @default.
• W4282559105 cites W2806864169 @default.
• W4282559105 cites W2911554128 @default.
• W4282559105 cites W2928665623 @default.
• W4282559105 cites W2958889289 @default.
• W4282559105 cites W3084191442 @default.
• W4282559105 cites W3099018540 @default.
• W4282559105 cites W3120325987 @default.
• W4282559105 cites W3146212824 @default.
• W4282559105 cites W3194345503 @default.
• W4282559105 cites W4293767581 @default.
• W4282559105 cites W4294541781 @default.
• W4282559105 doi "https://doi.org/10.1016/j.ygeno.2022.110403" @default.
• W4282559105 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35709926" @default.
• W4282559105 hasPublicationYear "2022" @default.
• W4282559105 type Work @default.
• W4282559105 citedByCount "1" @default.
• W4282559105 countsByYear W42825591052023 @default.
• W4282559105 crossrefType "journal-article" @default.
• W4282559105 hasAuthorship W4282559105A5002322160 @default.
• W4282559105 hasAuthorship W4282559105A5010738468 @default.
• W4282559105 hasAuthorship W4282559105A5012993508 @default.
• W4282559105 hasAuthorship W4282559105A5019985710 @default.
• W4282559105 hasAuthorship W4282559105A5027861145 @default.
• W4282559105 hasAuthorship W4282559105A5048542794 @default.
• W4282559105 hasAuthorship W4282559105A5049078993 @default.
• W4282559105 hasAuthorship W4282559105A5081456977 @default.
• W4282559105 hasBestOaLocation W42825591051 @default.
• W4282559105 hasConcept C104317684 @default.
• W4282559105 hasConcept C119857082 @default.
• W4282559105 hasConcept C142724271 @default.
• W4282559105 hasConcept C150194340 @default.
• W4282559105 hasConcept C152724338 @default.
• W4282559105 hasConcept C18431079 @default.
• W4282559105 hasConcept C2777538232 @default.
• W4282559105 hasConcept C2987395477 @default.
• W4282559105 hasConcept C41008148 @default.
• W4282559105 hasConcept C54355233 @default.
• W4282559105 hasConcept C58471807 @default.
• W4282559105 hasConcept C70721500 @default.
• W4282559105 hasConcept C71924100 @default.
• W4282559105 hasConcept C86803240 @default.
• W4282559105 hasConceptScore W4282559105C104317684 @default.
• W4282559105 hasConceptScore W4282559105C119857082 @default.
• W4282559105 hasConceptScore W4282559105C142724271 @default.
• W4282559105 hasConceptScore W4282559105C150194340 @default.
• W4282559105 hasConceptScore W4282559105C152724338 @default.
• W4282559105 hasConceptScore W4282559105C18431079 @default.
• W4282559105 hasConceptScore W4282559105C2777538232 @default.
• W4282559105 hasConceptScore W4282559105C2987395477 @default.
• W4282559105 hasConceptScore W4282559105C41008148 @default.
• W4282559105 hasConceptScore W4282559105C54355233 @default.
• W4282559105 hasConceptScore W4282559105C58471807 @default.
• W4282559105 hasConceptScore W4282559105C70721500 @default.
• W4282559105 hasConceptScore W4282559105C71924100 @default.
• W4282559105 hasConceptScore W4282559105C86803240 @default.

• next