Matches in SemOpenAlex for { <https://semopenalex.org/work/W4282587462> ?p ?o ?g. }
- W4282587462 endingPage "11" @default.
- W4282587462 startingPage "1" @default.
- W4282587462 abstract "Enhancing sperm motility in vitro has immensely benefited assisted conception methods. Phosphodiesterases (PDE) break the second messenger cAMP, and therefore, inhibition of their catalytic activity enhances the sperm motility through maintaining cAMP homeostasis in sperm. In view of identifying the molecules that could inhibit PDE functioning in spermatozoa, we aimed to evaluate the phosphodiesterase inhibitors (PDEI) - xanthine derivatives - acefylline, dyphylline and proxyphylline to repurpose them for assisted reproductive technology. These are available in the market as pharmaceutical agents to treat mainly respiratory system diseases. Based on the structure guided in silico studies, we predicted that these molecules bind to the cAMP binding catalytic pocket of PDE enzymes, and further molecular dynamics simulation analysis indicated that these molecules form the stable complexes. Isothermal titration calorimetry studies revealed that acefylline has better affinity towards PDE4A, PDE4D and PDE10A, when compared to dyphylline and proxyphylline. In addition, ex vivo studies corroborated in vitro binding studies that acefylline has much superior sperm motility enhancement property on human ejaculated spermatozoa and mouse testicular spermatozoa compared to dyphylline and proxyphylline.Communicated by Ramaswamy H. Sarma." @default.
- W4282587462 created "2022-06-14" @default.
- W4282587462 creator A5000976880 @default.
- W4282587462 creator A5018202119 @default.
- W4282587462 creator A5021040279 @default.
- W4282587462 creator A5027938643 @default.
- W4282587462 creator A5029798892 @default.
- W4282587462 creator A5037781049 @default.
- W4282587462 creator A5046564235 @default.
- W4282587462 creator A5070350230 @default.
- W4282587462 date "2022-06-13" @default.
- W4282587462 modified "2023-10-01" @default.
- W4282587462 title "Computational, biochemical and <i>ex vivo</i> evaluation of xanthine derivatives against phosphodiesterases to enhance the sperm motility" @default.
- W4282587462 cites W158637519 @default.
- W4282587462 cites W1967930073 @default.
- W4282587462 cites W1968554135 @default.
- W4282587462 cites W1972067660 @default.
- W4282587462 cites W1989014719 @default.
- W4282587462 cites W2007937891 @default.
- W4282587462 cites W2009513498 @default.
- W4282587462 cites W2010898616 @default.
- W4282587462 cites W2012155961 @default.
- W4282587462 cites W2038442892 @default.
- W4282587462 cites W2042697582 @default.
- W4282587462 cites W2061444940 @default.
- W4282587462 cites W2079195963 @default.
- W4282587462 cites W2079953901 @default.
- W4282587462 cites W2087102252 @default.
- W4282587462 cites W2106208364 @default.
- W4282587462 cites W2116919018 @default.
- W4282587462 cites W2117029355 @default.
- W4282587462 cites W2135779754 @default.
- W4282587462 cites W2137397057 @default.
- W4282587462 cites W2140627081 @default.
- W4282587462 cites W2144081679 @default.
- W4282587462 cites W2150333796 @default.
- W4282587462 cites W2152060247 @default.
- W4282587462 cites W2155085262 @default.
- W4282587462 cites W2157743162 @default.
- W4282587462 cites W2158269238 @default.
- W4282587462 cites W2164993311 @default.
- W4282587462 cites W2208775284 @default.
- W4282587462 cites W2273389178 @default.
- W4282587462 cites W2274492987 @default.
- W4282587462 cites W2279471239 @default.
- W4282587462 cites W2290720113 @default.
- W4282587462 cites W2431711966 @default.
- W4282587462 cites W2556912380 @default.
- W4282587462 cites W2736985130 @default.
- W4282587462 cites W2751637762 @default.
- W4282587462 cites W2890113953 @default.
- W4282587462 cites W2902947534 @default.
- W4282587462 cites W3171384822 @default.
- W4282587462 cites W4200136444 @default.
- W4282587462 doi "https://doi.org/10.1080/07391102.2022.2085802" @default.
- W4282587462 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35696450" @default.
- W4282587462 hasPublicationYear "2022" @default.
- W4282587462 type Work @default.
- W4282587462 citedByCount "2" @default.
- W4282587462 countsByYear W42825874622023 @default.
- W4282587462 crossrefType "journal-article" @default.
- W4282587462 hasAuthorship W4282587462A5000976880 @default.
- W4282587462 hasAuthorship W4282587462A5018202119 @default.
- W4282587462 hasAuthorship W4282587462A5021040279 @default.
- W4282587462 hasAuthorship W4282587462A5027938643 @default.
- W4282587462 hasAuthorship W4282587462A5029798892 @default.
- W4282587462 hasAuthorship W4282587462A5037781049 @default.
- W4282587462 hasAuthorship W4282587462A5046564235 @default.
- W4282587462 hasAuthorship W4282587462A5070350230 @default.
- W4282587462 hasConcept C104317684 @default.
- W4282587462 hasConcept C150903083 @default.
- W4282587462 hasConcept C156911925 @default.
- W4282587462 hasConcept C181199279 @default.
- W4282587462 hasConcept C185592680 @default.
- W4282587462 hasConcept C202751555 @default.
- W4282587462 hasConcept C207001950 @default.
- W4282587462 hasConcept C26291073 @default.
- W4282587462 hasConcept C2775905019 @default.
- W4282587462 hasConcept C2777632594 @default.
- W4282587462 hasConcept C2781087480 @default.
- W4282587462 hasConcept C54355233 @default.
- W4282587462 hasConcept C55493867 @default.
- W4282587462 hasConcept C58207958 @default.
- W4282587462 hasConcept C62826618 @default.
- W4282587462 hasConcept C86803240 @default.
- W4282587462 hasConcept C95444343 @default.
- W4282587462 hasConcept C98274493 @default.
- W4282587462 hasConceptScore W4282587462C104317684 @default.
- W4282587462 hasConceptScore W4282587462C150903083 @default.
- W4282587462 hasConceptScore W4282587462C156911925 @default.
- W4282587462 hasConceptScore W4282587462C181199279 @default.
- W4282587462 hasConceptScore W4282587462C185592680 @default.
- W4282587462 hasConceptScore W4282587462C202751555 @default.
- W4282587462 hasConceptScore W4282587462C207001950 @default.
- W4282587462 hasConceptScore W4282587462C26291073 @default.
- W4282587462 hasConceptScore W4282587462C2775905019 @default.
- W4282587462 hasConceptScore W4282587462C2777632594 @default.
- W4282587462 hasConceptScore W4282587462C2781087480 @default.