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- W4282827521 endingPage "1898" @default.
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- W4282827521 abstract "Preeclampsia (PE) is a pregnancy-specific disorder that affects 3 to 5% of pregnancies worldwide and is one of the leading causes of maternal and fetal morbidity and mortality. Nevertheless, how these events occur remains unclear. We hypothesized that the induction of hypoxic conditions in vitro in primary human trophoblast cells would mimic several characteristics of PE found in vivo. We applied and characterized a model of primary cytotrophoblasts isolated from healthy pregnancies that were placed under different oxygen concentrations: ambient O2 (5% pCO2, 21%pO2, 24 h, termed normoxia), low O2 concentration (5% pCO2, 1.5% pO2, 24 h, termed hypoxia), or hypoxia/reoxygenation (H/R: 6 h intervals of normoxia and hypoxia for 24 h). Various established preeclamptic markers were assessed in this cell model and compared to placental tissues obtained from PE pregnancies. Seventeen PE markers were analyzed by qPCR, and the protein secretion of soluble fms-like tyrosine kinase 1 (sFlT-1) and the placenta growth factor (PlGF) was determined by ELISA. Thirteen of seventeen genes associated with angiogenesis, the renin-angiotensin system, oxidative stress, endoplasmic reticulum stress, and the inflammasome complex were susceptible to H/R and hypoxia, mimicking the expression pattern of PE tissue. In cell culture supernatants, the secretion of sFlT-1 was increased in hypoxia, while PlGF release was significantly reduced in H/R and hypoxia. In the supernatants of our cell models, the sFlT-1/PlGF ratio in hypoxia and H/R was higher than 38, which is a strong indicator for PE in clinical practice. These results suggest that our cellular models reflect important pathological processes occurring in PE and are therefore suitable as PE in vitro models." @default.
- W4282827521 created "2022-06-15" @default.
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- W4282827521 date "2022-06-11" @default.
- W4282827521 modified "2023-10-18" @default.
- W4282827521 title "Primary Human Trophoblasts Mimic the Preeclampsia Phenotype after Acute Hypoxia–Reoxygenation Insult" @default.
- W4282827521 cites W167492261 @default.
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- W4282827521 cites W1971506857 @default.
- W4282827521 cites W1974704204 @default.
- W4282827521 cites W1976868429 @default.
- W4282827521 cites W1982391253 @default.
- W4282827521 cites W1990335913 @default.
- W4282827521 cites W1992608272 @default.
- W4282827521 cites W1993234733 @default.
- W4282827521 cites W1998476726 @default.
- W4282827521 cites W2017771409 @default.
- W4282827521 cites W2025420713 @default.
- W4282827521 cites W2032095810 @default.
- W4282827521 cites W2033000623 @default.
- W4282827521 cites W2033311273 @default.
- W4282827521 cites W2036520114 @default.
- W4282827521 cites W2050087753 @default.
- W4282827521 cites W2052252925 @default.
- W4282827521 cites W2053320849 @default.
- W4282827521 cites W2062091566 @default.
- W4282827521 cites W2062979358 @default.
- W4282827521 cites W2064551554 @default.
- W4282827521 cites W2067557867 @default.
- W4282827521 cites W2071210882 @default.
- W4282827521 cites W2081590724 @default.
- W4282827521 cites W2082154952 @default.
- W4282827521 cites W2084969086 @default.
- W4282827521 cites W2088098710 @default.
- W4282827521 cites W2101848184 @default.
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- W4282827521 cites W2105632053 @default.
- W4282827521 cites W2108609457 @default.
- W4282827521 cites W2112769664 @default.
- W4282827521 cites W2119682545 @default.
- W4282827521 cites W2122154463 @default.
- W4282827521 cites W2127451382 @default.
- W4282827521 cites W2137361939 @default.
- W4282827521 cites W2143180984 @default.
- W4282827521 cites W2145530551 @default.
- W4282827521 cites W2147512275 @default.
- W4282827521 cites W2152804345 @default.
- W4282827521 cites W2153354406 @default.
- W4282827521 cites W2165445226 @default.
- W4282827521 cites W2165954309 @default.
- W4282827521 cites W2170867031 @default.
- W4282827521 cites W2324187112 @default.
- W4282827521 cites W23403217 @default.
- W4282827521 cites W2533922325 @default.
- W4282827521 cites W2587261901 @default.
- W4282827521 cites W2608585389 @default.
- W4282827521 cites W2737043289 @default.
- W4282827521 cites W2746176044 @default.
- W4282827521 cites W2752391781 @default.
- W4282827521 cites W2774638683 @default.
- W4282827521 cites W2803051647 @default.
- W4282827521 cites W2803203272 @default.
- W4282827521 cites W2803702463 @default.
- W4282827521 cites W28037142 @default.
- W4282827521 cites W2807701456 @default.
- W4282827521 cites W2883123459 @default.
- W4282827521 cites W2919736910 @default.
- W4282827521 cites W2946902161 @default.
- W4282827521 cites W2995050802 @default.
- W4282827521 cites W3013289441 @default.
- W4282827521 cites W3014521923 @default.
- W4282827521 cites W3034255504 @default.
- W4282827521 cites W3182770627 @default.
- W4282827521 cites W3214476593 @default.
- W4282827521 cites W3216881676 @default.
- W4282827521 cites W4210253524 @default.
- W4282827521 cites W4220718289 @default.
- W4282827521 cites W4220815687 @default.
- W4282827521 cites W4242472190 @default.
- W4282827521 cites W4251118184 @default.
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- W4282827521 doi "https://doi.org/10.3390/cells11121898" @default.
- W4282827521 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35741027" @default.
- W4282827521 hasPublicationYear "2022" @default.
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